Restriction map for
pF1KE2188
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsgI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HhaI HinP1I HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI Tru9I Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 810 CGCG CG^CG
AcsI 1016 GAATTC [GA]^AATT[CT]
AcyI 974 GGCGTC G[GA]^CG[CT]C
AfaI 287 GTAC GT^AC
ApoI 1016 GAATTC [GA]^AATT[CT]
AspHI 760 GTGCTC G[AT]GC[AT]^C
BbrPI 774 CACGTG CAC^GTG
BbsI 28 GTCTTC GAAGAC(2/6)
BbvCI 358 GCTGAGG CCTCAGC(-5/-2)
BglII 1157 AGATCT A^GATCT
BpuAI 28 GTCTTC GAAGAC(2/6)
BsaAI 774 CACGTG [CT]AC^GT[GA]
BsaHI 974 GGCGTC G[GA]^CG[CT]C
BsaI 609 GGTCTC GGTCTC(1/5)
BsiHKAI 760 GTGCTC G[AT]GC[AT]^C
BsmBI 976 CGTCTC CGTCTC(1/5)
BsrBI 1236 CCGCTC CCGCTC(-3/-3)
BstEII 219 GGTCACC G^GT[ACGT]ACC
BstPI 219 GGTCACC G^GT[ACGT]ACC
BstUI 810 CGCG CG^CG
BstYI 1157 AGATCT [GA]^GATC[CT]
DsaI 401 CCGTGG C^C[GA][CT]GG
EcoO65I 219 GGTCACC G^GT[ACGT]ACC
EcoRI 1016 GAATTC G^AATTC
HgaI 975 GCGTC GACGC(5/10)
Hin1I 974 GGCGTC G[GA]^CG[CT]C
HincII 987 GTCGAC GT[CT]^[GA]AC
HindII 987 GTCGAC GT[CT]^[GA]AC
MflI 1157 AGATCT [GA]^GATC[CT]
MvnI 810 CGCG CG^CG
NspI 68 ACATGC [GA]CATG^[CT]
PflMI 1006 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 262 GAGTC GAGTC(4/5)
PmaCI 774 CACGTG CAC^GTG
PmlI 774 CACGTG CAC^GTG
PstI 582 CTGCAG CTGCA^G
RsaI 287 GTAC GT^AC
SalI 987 GTCGAC G^TCGAC
SapI 762 GCTCTTC GCTCTTC(1/4)
SfcI 582 CTGCAG C^T[GA][CT]AG
SspI 904 AATATT AAT^ATT
TfiI 367 GAATC G^A[AT]TC
Tsp509I 1017 AATT ^AATT
Van91I 1006 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1157 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwI 1002 GATCC GGATC(4/5)
848 GGATC
AlwNI 203 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
266 CAGGGGCTG
ApaI 20 GGGCCC GGGCC^C
859 GGGCCC
BcnI 234 CCCGG CC^[GC]GG
479 CCCGG
BseRI 136 GAGGAG GAGGAG(10/8)
361 GAGGAG
BsiEI 53 CGACCG CG[GA][CT]^CG
875 CGGTCG
BsmFI 818 GGGAC GGGAC(10/14)
1086 GGGAC
EarI 430 CTCTTC CTCTTC(1/4)
763 CTCTTC
FauI 1235 CCCGC CCCGC(4/6)
526 GCGGG
HinfI 262 GAGTC G^A[ACGT]TC
367 GAATC
Ksp632I 430 CTCTTC CTCTTC(1/4)
763 CTCTTC
NciI 234 CCCGG CC^[GC]GG
479 CCCGG
PpuMI 818 GGGACCT [GA]G^G[AT]CC[CT]
1207 AGGACCC
SmlI 520 CTCAAG C^T[CT][GA]AG
711 CTCAAG
TaqI 800 TCGA T^CGA
988 TCGA
Tsp45I 83 GTGAC ^GT[GC]AC
220 GTCAC
TthHB8I 800 TCGA T^CGA
988 TCGA
XcmI 146 CCAACACCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
440 CCATCACCATCCTGG
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccI AciI AluI AvaII BalI
BanII BbvI BmyI BpmI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PvuII Sau3AI
Sau96I ScrFI SfaNI TseI TspRI