Restriction map for
pF1KE6669
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BcgI
BciVI BclI BcnI BfaI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CfoI ClaI CpoI DraI
DraIII DrdI DsaI Eco47III Eco81I
EcoNI EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HphI KasI KpnI KspI
MaeI MaeIII MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NarI NciI NcoI NdeI NheI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tsp45I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AcsI 394 GAATTC [GA]^AATT[CT]
AhdI 603 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 861 GATCC GGATC(4/5)
AlwNI 322 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 394 GAATTC [GA]^AATT[CT]
AspEI 603 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 98 CCTCAGC CCTCAGC(-5/-2)
BbvI 814 GCTGC GCAGC(8/12)
BglI 50 GCCGAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 340 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 340 GCTGAGC GC^T[ACGT]AGC
BsaI 528 GGTCTC GGTCTC(1/5)
BsgI 835 CTGCAC GTGCAG(16/14)
BsiEI 314 CGGCCG CG[GA][CT]^CG
BsmFI 74 GGGAC GGGAC(10/14)
BspMI 431 ACCTGC ACCTGC(4/8)
BsrFI 55 GCCGGC [GA]^CCGG[CT]
BssSI 364 CACGAG CACGAG(-5/-1)
BstXI 634 CCACTGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 860 AGATCC [GA]^GATC[CT]
CelII 340 GCTGAGC GC^T[ACGT]AGC
Cfr10I 55 GCCGGC [GA]^CCGG[CT]
EagI 314 CGGCCG C^GGCCG
Eam1105I 603 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 314 CGGCCG C^GGCCG
Eco52I 314 CGGCCG C^GGCCG
EcoRI 394 GAATTC G^AATTC
EcoT14I 710 CCTTGG C^C[AT][AT]GG
HapII 56 CCGG C^CGG
HindIII 687 AAGCTT A^AGCTT
HpaII 56 CCGG C^CGG
MaeII 278 ACGT A^CGT
MflI 860 AGATCC [GA]^GATC[CT]
MslI 635 CACTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 56 CCGG C^CGG
NaeI 55 GCCGGC GCC^GGC
NgoMIV 55 GCCGGC G^CCGGC
NotI 313 GCGGCCGC GC^GGCCGC
NspI 372 GCATGT [GA]CATG^[CT]
PflMI 195 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 158 GAGTC GAGTC(4/5)
PstI 219 CTGCAG CTGCA^G
PvuII 458 CAGCTG CAG^CTG
SexAI 660 ACCTGGT A^CC[AT]GGT
SfcI 219 CTGCAG C^T[GA][CT]AG
SspI 869 AATATT AAT^ATT
StyI 710 CCTTGG C^C[AT][AT]GG
TaqI 130 TCGA T^CGA
TfiI 84 GAATC G^A[AT]TC
TseI 814 GCTGC G^C[AT]GC
Tsp509I 395 AATT ^AATT
TthHB8I 130 TCGA T^CGA
Van91I 195 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 860 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AspHI 139 GAGCTC G[AT]GC[AT]^C
228 GAGCTC
AspI 300 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
659 GACCTGGTC
AvaI 13 CCCGAG C^[CT]CG[GA]G
142 CTCGGG
AvaII 658 GGACC G^G[AT]CC
707 GGTCC
BpmI 231 CTCCAG CTGGAG(16/14)
613 CTGGAG
BsaJI 710 CCTTGG C^C[ACGT][ACGT]GG
843 CCTGGG
BseRI 448 CTCCTC GAGGAG(10/8)
367 GAGGAG
BsiHKAI 139 GAGCTC G[AT]GC[AT]^C
228 GAGCTC
BsmAI 616 GAGAC GTCTC(1/5)
529 GTCTC
BsmI 3 GAATGC GAATGC(1/-1)
496 GCATTC
BsoBI 13 CCCGAG C^[CT]CG[GA]G
142 CTCGGG
BsrBI 317 CCGCTC CCGCTC(-3/-3)
478 GAGCGG
BsrI 810 ACTGG ACTGG(1/-1)
792 CCAGT
DraII 657 AGGACCT [GA]G^G[ACGT]CC[CT]
706 GGGTCCT
EaeI 48 TGGCCG [CT]^GGCC[GA]
314 CGGCCG
EcoO109I 657 AGGACCT [GA]G^G[ACGT]CC[CT]
706 GGGTCCT
HinfI 84 GAATC G^A[ACGT]TC
158 GAGTC
MseI 263 TTAA T^TAA
685 TTAA
MspA1I 458 CAGCTG C[AC]G^C[GT]G
501 CAGCGG
NlaIV 58 GGCTCC GG[ACGT]^[ACGT]CC
706 GGGTCC
PflFI 300 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
659 GACCTGGTC
PpuMI 657 AGGACCT [GA]G^G[AT]CC[CT]
706 GGGTCCT
SacI 139 GAGCTC GAGCT^C
228 GAGCTC
SapI 475 GAAGAGC GCTCTTC(1/4)
571 GAAGAGC
Tru9I 263 TTAA T^TAA
685 TTAA
Tth111I 300 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
659 GACCTGGTC
XcmI 191 CCAGCCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
693 CCATTTCAAGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI BanII BmyI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII ItaI
Ksp632I MboI MboII MnlI MvaI
MwoI NdeII NlaIII Sau3AI Sau96I
ScrFI SfaNI TspRI