Restriction map for
pF1KE0597
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# NO CUT ENZYMES ( 79 enzymes) #
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AccI AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AspEI AspI
BfrI BsaBI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV KspI MamI MfeI
MluI MroI MunI MvnI NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SnaBI SphI Sse8387I SspBI
SwaI Tth111I Van91I XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 2220 GACGTC GACGT^C
Acc65I 3071 GGTACC G^GTACC
AocI 3025 CCTCAGG CC^T[ACGT]AGG
AseI 568 ATTAAT AT^TAAT
AsnI 568 ATTAAT AT^TAAT
Asp718I 3071 GGTACC G^GTACC
AvrII 1381 CCTAGG C^CTAGG
BbrPI 3496 CACGTG CAC^GTG
BcgI 580 GCACGTAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4136 TGATCA T^GATCA
BglI 3295 GCCCTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1381 CCTAGG C^CTAGG
BsaWI 2261 ACCGGA [AT]^CCGG[AT]
BsmBI 1537 CGTCTC CGTCTC(1/5)
BspMI 1167 ACCTGC ACCTGC(4/8)
BsrDI 1434 CATTGC GCAATG(2/0)
BssSI 1352 CACGAG CACGAG(-5/-1)
BstXI 1832 CCATGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3025 CCTCAGG CC^T[ACGT]AGG
DraI 3153 TTTAAA TTT^AAA
DraII 1384 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 2827 GACCACACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3025 CCTCAGG CC^T[ACGT]AGG
EcoNI 1153 CCTAAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1384 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 779 ATGCAT ATGCA^T
FbaI 4136 TGATCA T^GATCA
FseI 3644 GGCCGGCC GGCCGG^CC
HgaI 3878 GCGTC GACGC(5/10)
HpaI 1752 GTTAAC GTT^AAC
KpnI 3071 GGTACC GGTAC^C
NdeI 4343 CATATG CA^TATG
NsiI 779 ATGCAT ATGCA^T
PmaCI 3496 CACGTG CAC^GTG
PmlI 3496 CACGTG CAC^GTG
PpuMI 1384 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 568 ATTAAT AT^TAAT
ScaI 160 AGTACT AGT^ACT
SpeI 2685 ACTAGT A^CTAGT
SspI 1634 AATATT AAT^ATT
StuI 89 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 28 enzymes) #
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Asp700I 268 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2652 GAAAGCTTTC
AviII 2087 TGCGCA TGC^GCA
2693 TGCGCA
BamHI 931 GGATCC G^GATCC
2264 GGATCC
BbeI 3 GGCGCC GGCGC^C
3908 GGCGCC
BbvCI 3973 GCTGAGG CCTCAGC(-5/-2)
4243 GCTGAGG
BciVI 1788 GGATAC GTATCC(6/5)
1887 GTATCC
BglII 2092 AGATCT A^GATCT
2240 AGATCT
BlpI 217 GCTGAGC GC^T[ACGT]AGC
4275 GCTAAGC
Bpu1102I 217 GCTGAGC GC^T[ACGT]AGC
4275 GCTAAGC
BsmI 1774 GCATTC GAATGC(1/-1)
2055 GCATTC
BspHI 329 TCATGA T^CATGA
2270 TCATGA
BsrFI 3645 GCCGGC [GA]^CCGG[CT]
3905 GCCGGC
CelII 217 GCTGAGC GC^T[ACGT]AGC
4275 GCTAAGC
Cfr10I 3645 GCCGGC [GA]^CCGG[CT]
3905 GCCGGC
FspI 2087 TGCGCA TGC^GCA
2693 TGCGCA
HincII 1752 GTTAAC GT[CT]^[GA]AC
3044 GTCAAC
HindII 1752 GTTAAC GT[CT]^[GA]AC
3044 GTCAAC
HindIII 256 AAGCTT A^AGCTT
2654 AAGCTT
KasI 3 GGCGCC G^GCGCC
3908 GGCGCC
NaeI 3645 GCCGGC GCC^GGC
3905 GCCGGC
NarI 3 GGCGCC GG^CGCC
3908 GGCGCC
NcoI 1832 CCATGG C^CATGG
4148 CCATGG
NgoMIV 3645 GCCGGC G^CCGGC
3905 GCCGGC
RcaI 329 TCATGA T^CATGA
2270 TCATGA
SacI 1532 GAGCTC GAGCT^C
4008 GAGCTC
SfoI 3 GGCGCC GGC^GCC
3908 GGCGCC
XbaI 1095 TCTAGA T^CTAGA
1584 TCTAGA
XmnI 268 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2652 GAAAGCTTTC
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# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI