Restriction map for
pF1KE3342
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# NO CUT ENZYMES ( 70 enzymes) #
##################################
AclI AflII AgeI Aor51HI AscI
AseI AsnI Asp700I AvrII BamHI
BfrI BlnI BsiEI BsiWI BsmBI
Bsp1407I BspLU11I BsrGI Bst1107I BstBI
BstZ17I CpoI DraI EagI EclXI
Eco47III Eco52I EcoRI EcoRV EcoT14I
EcoT22I FseI HindIII HpaI MfeI
MluI MseI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI StyI
SwaI Tru9I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 1458 GACGTC GACGT^C
AccI 1765 GTCTAC GT^[AC][GT]AC
AcsI 1813 GAATTT [GA]^AATT[CT]
AflIII 254 ACGTGT A^C[GA][CT]GT
AhdI 486 GACGCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 950 GTGCAC G^TGCAC
AlwNI 2786 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 950 GTGCAC G^TGCAC
ApoI 1813 GAATTT [GA]^AATT[CT]
AspEI 486 GACGCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1326 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 164 TGCGCA TGC^GCA
BbrPI 253 CACGTG CAC^GTG
BciVI 1234 GTATCC GTATCC(6/5)
BclI 2486 TGATCA T^GATCA
BfaI 2239 CTAG C^TAG
BglII 2822 AGATCT A^GATCT
BlpI 504 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 504 GCTCAGC GC^T[ACGT]AGC
BsaAI 253 CACGTG [CT]AC^GT[GA]
BsaBI 2343 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2583 GAATGC GAATGC(1/-1)
BspDI 1828 ATCGAT AT^CGAT
BspHI 2066 TCATGA T^CATGA
BssHII 1525 GCGCGC G^CGCGC
BstAPI 156 GCACAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2046 GGTCACC G^GT[ACGT]ACC
BstPI 2046 GGTCACC G^GT[ACGT]ACC
BstYI 2822 AGATCT [GA]^GATC[CT]
CelII 504 GCTCAGC GC^T[ACGT]AGC
ClaI 1828 ATCGAT AT^CGAT
DrdI 1396 GACTACGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 486 GACGCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2046 GGTCACC G^GT[ACGT]ACC
FbaI 2486 TGATCA T^GATCA
FspI 164 TGCGCA TGC^GCA
HincII 2242 GTCAAC GT[CT]^[GA]AC
HindII 2242 GTCAAC GT[CT]^[GA]AC
MaeI 2239 CTAG C^TAG
MamI 2343 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 2822 AGATCT [GA]^GATC[CT]
NaeI 1537 GCCGGC GCC^GGC
NgoMIV 1537 GCCGGC G^CCGGC
PaeR7I 283 CTCGAG C^TCGAG
PflFI 1326 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 253 CACGTG CAC^GTG
PmlI 253 CACGTG CAC^GTG
RcaI 2066 TCATGA T^CATGA
SspI 1718 AATATT AAT^ATT
StuI 1991 AGGCCT AGG^CCT
Tth111I 1326 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 283 CTCGAG C^TCGAG
XhoII 2822 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 1296 GGTACC G^GTACC
2186 GGTACC
AccIII 2381 TCCGGA T^CCGGA
2633 TCCGGA
AocI 106 CCTCAGG CC^T[ACGT]AGG
1686 CCTCAGG
ApaI 1252 GGGCCC GGGCC^C
1546 GGGCCC
Asp718I 1296 GGTACC G^GTACC
2186 GGTACC
BalI 726 TGGCCA TGG^CCA
2885 TGGCCA
BbvCI 784 GCTGAGG CCTCAGC(-5/-2)
2830 GCTGAGG
BcgI 1628 GCACGGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1714 GCAGAATATTCG
BseAI 2381 TCCGGA T^CCGGA
2633 TCCGGA
BseRI 1008 CTCCTC GAGGAG(10/8)
2296 GAGGAG
BspEI 2381 TCCGGA T^CCGGA
2633 TCCGGA
BsrDI 2373 CATTGC GCAATG(2/0)
1700 GCAATG
BssSI 2322 CACGAG CACGAG(-5/-1)
2149 CTCGTG
BstXI 1502 CCAGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2753 CCATCAAAATGG
Bsu36I 106 CCTCAGG CC^T[ACGT]AGG
1686 CCTCAGG
Eco81I 106 CCTCAGG CC^T[ACGT]AGG
1686 CCTCAGG
EcoNI 141 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1509 CCTGGTGCAGG
KpnI 1296 GGTACC GGTAC^C
2186 GGTACC
KspI 425 CCGCGG CCGC^GG
979 CCGCGG
MluNI 726 TGGCCA TGG^CCA
2885 TGGCCA
MroI 2381 TCCGGA T^CCGGA
2633 TCCGGA
MscI 726 TGGCCA TGG^CCA
2885 TGGCCA
PflMI 2145 CCAGCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2348 CCATCCGATGG
PstI 756 CTGCAG CTGCA^G
1304 CTGCAG
SacI 4 GAGCTC GAGCT^C
2325 GAGCTC
SacII 425 CCGCGG CCGC^GG
979 CCGCGG
SexAI 1154 ACCTGGT A^CC[AT]GGT
1508 ACCTGGT
SfcI 756 CTGCAG C^T[GA][CT]AG
1304 CTGCAG
Tsp509I 71 AATT ^AATT
1814 AATT
Van91I 2145 CCAGCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2348 CCATCCGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BanI
BanII BbeI BbsI BbvI BcnI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfoI SmaI
SmlI SphI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XmaCI XmaI