Restriction map for
pF1KE2335
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# NO CUT ENZYMES ( 91 enzymes) #
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AatII Acc65I AclI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspEI AvaI AvrII BanII
BbeI BbvCI BlnI BsaBI BsiEI
BsiWI BsmBI BsoBI BspLU11I BsrBI
BsrFI BssHII BssSI BstAPI BstBI
BstEII BstPI Cfr10I CpoI DraII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRV EcoT22I
FauI FseI KasI KpnI KspI
MamI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NsiI NspV PacI PaeR7I PinAI
PmeI PpuMI PshAI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccII 357 CGCG CG^CG
AccIII 998 TCCGGA T^CCGGA
AcyI 660 GGCGTC G[GA]^CG[CT]C
AflII 861 CTTAAG C^TTAAG
AspHI 4118 GTGCTC G[AT]GC[AT]^C
AspI 4006 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1037 TGCGCA TGC^GCA
BanI 1498 GGCACC G^G[CT][GA]CC
BbrPI 3589 CACGTG CAC^GTG
BcgI 4091 CGAAGATCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 330 CCCGG CC^[GC]GG
BfrI 861 CTTAAG C^TTAAG
BglI 2389 GCCCCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 920 GCTCAGC GC^T[ACGT]AGC
BmyI 4118 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 920 GCTCAGC GC^T[ACGT]AGC
BsaHI 660 GGCGTC G[GA]^CG[CT]C
BseAI 998 TCCGGA T^CCGGA
BsgI 1961 GTGCAG GTGCAG(16/14)
BsiHKAI 4118 GTGCTC G[AT]GC[AT]^C
BsmFI 1882 GGGAC GGGAC(10/14)
Bsp1286I 4118 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 1071 TGTACA T^GTACA
BspDI 3805 ATCGAT AT^CGAT
BspEI 998 TCCGGA T^CCGGA
BspHI 3416 TCATGA T^CATGA
BspMI 2905 GCAGGT ACCTGC(4/8)
BsrGI 1071 TGTACA T^GTACA
Bst1107I 1684 GTATAC GTA^TAC
BstUI 357 CGCG CG^CG
BstXI 3801 CCAGATCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1684 GTATAC GTA^TAC
CelII 920 GCTCAGC GC^T[ACGT]AGC
ClaI 3805 ATCGAT AT^CGAT
DsaI 4041 CCATGG C^C[GA][CT]GG
EcoNI 702 CCTTTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1037 TGCGCA TGC^GCA
HaeII 89 AGCGCC [GA]GCGC^[CT]
Hin1I 660 GGCGTC G[GA]^CG[CT]C
HpaI 2329 GTTAAC GTT^AAC
MroI 998 TCCGGA T^CCGGA
MspA1I 51 CAGCTG C[AC]G^C[GT]G
MvnI 357 CGCG CG^CG
NciI 330 CCCGG CC^[GC]GG
NcoI 4041 CCATGG C^CATGG
NruI 356 TCGCGA TCG^CGA
PflFI 4006 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3711 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3589 CACGTG CAC^GTG
PmlI 3589 CACGTG CAC^GTG
PvuII 51 CAGCTG CAG^CTG
RcaI 3416 TCATGA T^CATGA
SphI 1567 GCATGC GCATG^C
SspBI 1071 TGTACA T^GTACA
Tth111I 4006 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3711 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 513 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccI 1684 GTATAC GT^[AC][GT]AC
2801 GTCTAC
AflIII 2894 ACACGT A^C[GA][CT]GT
3588 ACACGT
AocI 2392 CCTCAGG CC^T[ACGT]AGG
3614 CCTCAGG
AvaII 56 GGACC G^G[AT]CC
2562 GGTCC
BbsI 486 GAAGAC GAAGAC(2/6)
2479 GAAGAC
BciVI 79 GGATAC GTATCC(6/5)
1726 GGATAC
BclI 2261 TGATCA T^GATCA
3933 TGATCA
BpuAI 486 GAAGAC GAAGAC(2/6)
2479 GAAGAC
BsaAI 2755 TACGTG [CT]AC^GT[GA]
3589 CACGTG
BsaI 1990 GAGACC GGTCTC(1/5)
3967 GGTCTC
BsaWI 998 TCCGGA [AT]^CCGG[AT]
4355 ACCGGA
BsmI 446 GAATGC GAATGC(1/-1)
4225 GAATGC
Bsu36I 2392 CCTCAGG CC^T[ACGT]AGG
3614 CCTCAGG
DraIII 1640 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3191 CACTTCGTG
Eco81I 2392 CCTCAGG CC^T[ACGT]AGG
3614 CCTCAGG
EcoRI 2094 GAATTC G^AATTC
3582 GAATTC
FbaI 2261 TGATCA T^GATCA
3933 TGATCA
HgaI 661 GCGTC GACGC(5/10)
1556 GCGTC
HindIII 3171 AAGCTT A^AGCTT
4000 AAGCTT
NspI 1567 GCATGC [GA]CATG^[CT]
3503 ACATGC
ScaI 2683 AGTACT AGT^ACT
3751 AGTACT
XcmI 3446 CCAGCATAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4115 CCAGTGCTCATTTGG
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
BalI BamHI BbvI BfaI BglII
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI