Restriction map for
pF1KE2379
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# NO CUT ENZYMES ( 85 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp718I
AspEI AvrII BamHI BbvCI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaBI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BsrDI BsrGI Bst1107I
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoRI EcoRV EcoT22I
FbaI HincII HindII HindIII HpaI
KpnI MamI MfeI MluI MroI
MseI MunI NdeI NotI NruI
NsiI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RsrII SalI
SexAI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI Tru9I XbaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 2377 GACGTC GACGT^C
Alw44I 1662 GTGCAC G^TGCAC
ApaLI 1662 GTGCAC G^TGCAC
AspI 2377 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 932 TGCGCA TGC^GCA
BbrPI 661 CACGTG CAC^GTG
BcgI 817 CGAGAAAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1913 AGATCT A^GATCT
BsaAI 661 CACGTG [CT]AC^GT[GA]
BsaI 3157 GAGACC GGTCTC(1/5)
BsaWI 296 ACCGGA [AT]^CCGG[AT]
BspHI 426 TCATGA T^CATGA
BspLU11I 2528 ACATGT A^CATGT
BssSI 970 CTCGTG CACGAG(-5/-1)
BstEII 1119 GGTCACC G^GT[ACGT]ACC
BstPI 1119 GGTCACC G^GT[ACGT]ACC
BstXI 455 CCAGCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EarI 2934 GAAGAG CTCTTC(1/4)
EcoO65I 1119 GGTCACC G^GT[ACGT]ACC
FseI 2761 GGCCGGCC GGCCGG^CC
FspI 932 TGCGCA TGC^GCA
Ksp632I 2934 GAAGAG CTCTTC(1/4)
NaeI 2762 GCCGGC GCC^GGC
NcoI 650 CCATGG C^CATGG
NgoMIV 2762 GCCGGC G^CCGGC
PaeR7I 2089 CTCGAG C^TCGAG
PflFI 2377 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 661 CACGTG CAC^GTG
PmlI 661 CACGTG CAC^GTG
PshAI 3213 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 426 TCATGA T^CATGA
SacI 3031 GAGCTC GAGCT^C
SapI 2934 GAAGAGC GCTCTTC(1/4)
SbfI 2169 CCTGCAGG CCTGCA^GG
ScaI 2096 AGTACT AGT^ACT
SphI 2310 GCATGC GCATG^C
Sse8387I 2169 CCTGCAGG CCTGCA^GG
StuI 2440 AGGCCT AGG^CCT
TfiI 996 GATTC G^A[AT]TC
Tsp509I 382 AATT ^AATT
Tth111I 2377 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2089 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AflIII 1973 ACGTGT A^C[GA][CT]GT
2528 ACATGT
AspHI 1662 GTGCAC G[AT]GC[AT]^C
3031 GAGCTC
BbsI 1572 GAAGAC GAAGAC(2/6)
1267 GTCTTC
BfaI 1690 CTAG C^TAG
1958 CTAG
BpuAI 1572 GAAGAC GAAGAC(2/6)
1267 GTCTTC
BsiHKAI 1662 GTGCAC G[AT]GC[AT]^C
3031 GAGCTC
BsmBI 1887 CGTCTC CGTCTC(1/5)
1303 GAGACG
BsrBI 2199 CCGCTC CCGCTC(-3/-3)
688 GAGCGG
BsrFI 3 GCCGGT [GA]^CCGG[CT]
2762 GCCGGC
BssHII 728 GCGCGC G^CGCGC
873 GCGCGC
BstAPI 249 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
924 GCACCCACTGC
Cfr10I 3 GCCGGT [GA]^CCGG[CT]
2762 GCCGGC
KspI 605 CCGCGG CCGC^GG
680 CCGCGG
MaeI 1690 CTAG C^TAG
1958 CTAG
NheI 1689 GCTAGC G^CTAGC
1957 GCTAGC
SacII 605 CCGCGG CCGC^GG
680 CCGCGG
SfiI 1801 GGCCTGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2765 GGCCGGGGGGGCC
SmlI 1027 CTCAAG C^T[CT][GA]AG
2089 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI Asp700I AvaI
AvaII BalI BanI BanII BbeI
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiEI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI