Restriction map for
pF1KB8607
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BalI BamHI BbeI BbrPI BclI
BcnI BfrI BlnI BlpI Bpu1102I
BsaBI BseAI BsgI BsiEI BsiWI
BsmBI BspDI BspEI BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HhaI HinP1I HpaI KasI KpnI
KspI MamI MluI MluNI MroI
MscI MvnI NaeI NarI NciI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 250 GTCTAC GT^[AC][GT]AC
AcyI 13 GGCGTC G[GA]^CG[CT]C
ApaI 1201 GGGCCC GGGCC^C
AspI 772 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 49 GGTGCC G^G[CT][GA]CC
BanII 1201 GGGCCC G[GA]GC[CT]^C
BbsI 1813 GAAGAC GAAGAC(2/6)
BbvCI 223 GCTGAGG CCTCAGC(-5/-2)
BcgI 873 CGAGTTCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 165 GGATAC GTATCC(6/5)
BglI 721 GCCCCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1813 GAAGAC GAAGAC(2/6)
BsaAI 655 TACGTG [CT]AC^GT[GA]
BsaHI 13 GGCGTC G[GA]^CG[CT]C
BsaI 1118 GAGACC GGTCTC(1/5)
BsaWI 1571 ACCGGA [AT]^CCGG[AT]
BsmI 846 GAATGC GAATGC(1/-1)
BspHI 1412 TCATGA T^CATGA
BstAPI 30 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1761 CCACATGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
FauI 64 CCCGC CCCGC(4/6)
HgaI 14 GCGTC GACGC(5/10)
Hin1I 13 GGCGTC G[GA]^CG[CT]C
HincII 711 GTTGAC GT[CT]^[GA]AC
HindII 711 GTTGAC GT[CT]^[GA]AC
HindIII 327 AAGCTT A^AGCTT
MaeII 656 ACGT A^CGT
MfeI 1474 CAATTG C^AATTG
MunI 1474 CAATTG C^AATTG
NdeI 1678 CATATG CA^TATG
NheI 1149 GCTAGC G^CTAGC
PflFI 772 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 684 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 37 CTGCAG CTGCA^G
RcaI 1412 TCATGA T^CATGA
SgrAI 45 CGCCGGTG C[GA]^CCGG[CT]G
Tth111I 772 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 684 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcsI 412 AAATTC [GA]^AATT[CT]
1972 AAATTT
ApoI 412 AAATTC [GA]^AATT[CT]
1972 AAATTT
Asp700I 675 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1921 GAAGCCATTC
AspHI 90 GAGCAC G[AT]GC[AT]^C
1874 GAGCAC
AvaI 794 CTCGGG C^[CT]CG[GA]G
1082 CCCGAG
BglII 1038 AGATCT A^GATCT
1325 AGATCT
BpmI 1115 CTGGAG CTGGAG(16/14)
1517 CTGGAG
BsiHKAI 90 GAGCAC G[AT]GC[AT]^C
1874 GAGCAC
BsoBI 794 CTCGGG C^[CT]CG[GA]G
1082 CCCGAG
Bsp1407I 1546 TGTACA T^GTACA
1661 TGTACA
BspMI 59 ACCTGC ACCTGC(4/8)
1145 ACCTGC
BsrFI 46 GCCGGT [GA]^CCGG[CT]
490 ACCGGC
BsrGI 1546 TGTACA T^GTACA
1661 TGTACA
Cfr10I 46 GCCGGT [GA]^CCGG[CT]
490 ACCGGC
DraI 135 TTTAAA TTT^AAA
1263 TTTAAA
HphI 1849 GGTGA GGTGA(8/7)
1376 TCACC
MaeIII 509 GTGAC ^GT[ACGT]AC
1274 GTGAC
NcoI 725 CCATGG C^CATGG
893 CCATGG
PpuMI 70 GGGTCCT [GA]G^G[AT]CC[CT]
1983 AGGACCC
SfaNI 1362 GATGC GCATC(5/9)
461 GCATC
SfcI 37 CTGCAG C^T[GA][CT]AG
1127 CTACAG
SspBI 1546 TGTACA T^GTACA
1661 TGTACA
StuI 1670 AGGCCT AGG^CCT
1714 AGGCCT
TfiI 552 GAATC G^A[AT]TC
984 GAATC
Tsp45I 509 GTGAC ^GT[GC]AC
1274 GTGAC
XmnI 675 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1921 GAAGCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AvaII BbvI BfaI BmyI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BspLU11I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PvuII RsaI Sau3AI
Sau96I ScrFI StyI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I XcmI
XhoII