Restriction map for
pF1KE0940
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII BalI
BanI BbeI BbrPI BbsI BciVI
BcnI BfrI BglI BglII BlpI
Bpu1102I BpuAI BsaBI BsaHI BseAI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BsrBI BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRI
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI KspI
MaeIII MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tsp45I Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AlwNI 304 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 995 CCTTAGG CC^T[ACGT]AGG
AvrII 216 CCTAGG C^CTAGG
BamHI 56 GGATCC G^GATCC
BbvCI 283 GCTGAGG CCTCAGC(-5/-2)
BcgI 636 CGACCGAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 216 CCTAGG C^CTAGG
BpmI 1106 CTCCAG CTGGAG(16/14)
BsaAI 814 TACGTG [CT]AC^GT[GA]
BsaWI 478 TCCGGT [AT]^CCGG[AT]
BsiEI 636 CGACCG CG[GA][CT]^CG
BsmI 977 GAATGC GAATGC(1/-1)
Bsp1407I 362 TGTACA T^GTACA
BsrFI 1366 ACCGGC [GA]^CCGG[CT]
BsrGI 362 TGTACA T^GTACA
BsrI 517 ACTGG ACTGG(1/-1)
BstAPI 303 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 56 GGATCC [GA]^GATC[CT]
Bsu36I 995 CCTTAGG CC^T[ACGT]AGG
Cfr10I 1366 ACCGGC [GA]^CCGG[CT]
DsaI 689 CCATGG C^C[GA][CT]GG
EarI 694 GAAGAG CTCTTC(1/4)
Eco81I 995 CCTTAGG CC^T[ACGT]AGG
EcoNI 134 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1101 GATATC GAT^ATC
HaeIII 385 GGCC GG^CC
HindIII 1147 AAGCTT A^AGCTT
HphI 1197 TCACC GGTGA(8/7)
Ksp632I 694 GAAGAG CTCTTC(1/4)
MflI 56 GGATCC [GA]^GATC[CT]
NcoI 689 CCATGG C^CATGG
PflMI 810 CCAATACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 68 GAGTC GAGTC(4/5)
SapI 694 GAAGAGC GCTCTTC(1/4)
SfaNI 232 GATGC GCATC(5/9)
SspBI 362 TGTACA T^GTACA
Van91I 810 CCAATACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 56 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 363 GTAC GT^AC
565 GTAC
AlwI 57 GATCC GGATC(4/5)
56 GGATC
BanII 853 GAGCTC G[GA]GC[CT]^C
1342 GAGCTC
BclI 495 TGATCA T^GATCA
867 TGATCA
BfaI 217 CTAG C^TAG
1012 CTAG
BsaI 406 GAGACC GGTCTC(1/5)
1210 GAGACC
BsgI 700 CTGCAC GTGCAG(16/14)
289 GTGCAG
BspMI 443 ACCTGC ACCTGC(4/8)
1281 GCAGGT
FbaI 495 TGATCA T^GATCA
867 TGATCA
HapII 479 CCGG C^CGG
1367 CCGG
HpaII 479 CCGG C^CGG
1367 CCGG
MaeI 217 CTAG C^TAG
1012 CTAG
MslI 724 CACACCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1198 CACCTGGATG
MspI 479 CCGG C^CGG
1367 CCGG
NlaIV 56 GGATCC GG[ACGT]^[ACGT]CC
107 GGAGCC
PvuII 706 CAGCTG CAG^CTG
1057 CAGCTG
RsaI 363 GTAC GT^AC
565 GTAC
SacI 853 GAGCTC GAGCT^C
1342 GAGCTC
TaqI 473 TCGA T^CGA
635 TCGA
TthHB8I 473 TCGA T^CGA
635 TCGA
XcmI 806 CCATCCAATACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1108 CCAGACCGAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcsI AflIII AluI ApoI
AspHI BbvI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspLU11I BssKI BstF5I BstNI
Cac8I DdeI DpnII EcoRII EcoT14I
Fnu4HI FokI HinfI ItaI MaeII
MboI MboII MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NspI
PstI Sau3AI ScrFI SfcI StyI
TfiI Tru9I TseI Tsp509I TspRI