Restriction map for
pF1KE2626
##################################
# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BglI
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII HphI KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI TaqI TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AciI 529 GCGG CCGC(-3/-1)
AflII 346 CTTAAG C^TTAAG
Alw44I 707 GTGCAC G^TGCAC
AlwNI 135 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 707 GTGCAC G^TGCAC
AspHI 707 GTGCAC G[AT]GC[AT]^C
AspI 613 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 733 GGTCC G^G[AT]CC
BfrI 346 CTTAAG C^TTAAG
BglII 873 AGATCT A^GATCT
BmyI 707 GTGCAC G[AGT]GC[ACT]^C
BsaBI 66 GATAGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 707 GTGCAC G[AT]GC[AT]^C
BsiYI 697 CCTGTGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 697 CCTGTGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 143 GGGAC GGGAC(10/14)
Bsp1286I 707 GTGCAC G[AGT]GC[ACT]^C
BspMI 703 GCAGGT ACCTGC(4/8)
BsrDI 606 CATTGC GCAATG(2/0)
BsrI 552 ACTGG ACTGG(1/-1)
BssKI 84 CCTGG ^CC[ACGT]GG
BstAPI 766 GCAGGAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 243 GGATG GGATG(2/0)
BstNI 84 CCTGG CC^[AT]GG
Cfr13I 733 GGTCC G^G[ACGT]CC
DraII 732 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 782 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 697 CCTGTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 732 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 579 GAATTC G^AATTC
EcoRII 84 CCTGG ^CC[AT]GG
FokI 243 GGATG GGATG(9/13)
HaeIII 742 GGCC GG^CC
HincII 619 GTCAAC GT[CT]^[GA]AC
HindII 619 GTCAAC GT[CT]^[GA]AC
MaeII 177 ACGT A^CGT
MaeIII 556 GTCAC ^GT[ACGT]AC
MamI 66 GATAGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 163 CATATCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 84 CCTGG CC^[AT]GG
PflFI 613 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 459 GACTC GAGTC(4/5)
PpuMI 732 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 503 CTGCAG CTGCA^G
SapI 157 GAAGAGC GCTCTTC(1/4)
Sau96I 733 GGTCC G^G[ACGT]CC
ScaI 252 AGTACT AGT^ACT
ScrFI 84 CCTGG CC^[ACGT]GG
SfaNI 292 GCATC GCATC(5/9)
SspI 112 AATATT AAT^ATT
Tsp45I 556 GTCAC ^GT[GC]AC
Tth111I 613 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 9 enzymes) #
###################################
AfaI 179 GTAC GT^AC
253 GTAC
BsgI 779 CTGCAC GTGCAG(16/14)
132 GTGCAG
Cac8I 684 GCTGGC GC[ACGT]^[ACGT]GC
751 GCAGGC
MspA1I 776 CAGCTG C[AC]G^C[GT]G
864 CAGCTG
NlaIV 649 GGAACC GG[ACGT]^[ACGT]CC
732 GGGTCC
PvuII 776 CAGCTG CAG^CTG
864 CAGCTG
RsaI 179 GTAC GT^AC
253 GTAC
SmlI 346 CTTAAG C^T[CT][GA]AG
488 CTCAAG
TspRI 558 CACTG CA[GC]TG[ACGT][ACGT]^
624 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BfaI BstYI DdeI DpnII EarI
Fnu4HI HinfI ItaI Ksp632I MaeI
MboI MboII MflI MnlI MseI
MwoI NdeII NlaIII Sau3AI SfcI
TfiI Tru9I TseI Tsp509I XhoII