################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaHI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeII MfeI MluI MluNI MroI MscI MseI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcsI 160 GAATTC [GA]^AATT[CT] AfaI 138 GTAC GT^AC ApoI 160 GAATTC [GA]^AATT[CT] Asp700I 156 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 190 GGATCC G^GATCC BfaI 5 CTAG C^TAG BpmI 250 CTCCAG CTGGAG(16/14) BsaBI 240 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 226 GAGACC GGTCTC(1/5) BsaWI 89 ACCGGA [AT]^CCGG[AT] BssKI 231 CCAGG ^CC[ACGT]GG BstAPI 27 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 231 CCAGG CC^[AT]GG BstYI 190 GGATCC [GA]^GATC[CT] Cfr13I 72 GGCCC G^G[ACGT]CC DpnII 191 GATC ^GATC DsaI 195 CCGTGG C^C[GA][CT]GG EaeI 319 CGGCCA [CT]^GGCC[GA] EcoRI 160 GAATTC G^AATTC EcoRII 231 CCAGG ^CC[AT]GG EcoT14I 170 CCAAGG C^C[AT][AT]GG HinfI 21 GATTC G^A[ACGT]TC MaeI 5 CTAG C^TAG MaeIII 311 GTGAC ^GT[ACGT]AC MamI 240 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 191 GATC ^GATC MflI 190 GGATCC [GA]^GATC[CT] MslI 210 CACTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 28 CAGCTG C[AC]G^C[GT]G MvaI 231 CCAGG CC^[AT]GG NdeII 191 GATC ^GATC NlaIII 331 CATG CATG^ NlaIV 190 GGATCC GG[ACGT]^[ACGT]CC PvuII 28 CAGCTG CAG^CTG RsaI 138 GTAC GT^AC Sau3AI 191 GATC ^GATC Sau96I 72 GGCCC G^G[ACGT]CC ScrFI 231 CCAGG CC^[ACGT]GG StyI 170 CCAAGG C^C[AT][AT]GG TfiI 21 GATTC G^A[AT]TC Tsp45I 311 GTGAC ^GT[GC]AC TspRI 210 CACTG CA[GC]TG[ACGT][ACGT]^ XhoII 190 GGATCC [GA]^GATC[CT] XmnI 156 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AlwI 191 GATCC GGATC(4/5) 190 GGATC AlwNI 25 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 28 CAGCTGCTG BsiYI 41 CCGAGAAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 144 CCTGCAGAGGG BslI 41 CCGAGAAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 144 CCTGCAGAGGG BsmAI 226 GAGAC GTCTC(1/5) 12 GTCTC BstF5I 246 CATCC GGATG(2/0) 276 GGATG DdeI 14 CTCAG C^T[ACGT]AG 119 CTCAG FauI 74 CCCGC CCCGC(4/6) 48 GCGGG FokI 246 CATCC GGATG(9/13) 276 GGATG HapII 90 CCGG C^CGG 264 CCGG HpaII 90 CCGG C^CGG 264 CCGG MboII 112 GAAGA GAAGA(8/7) 237 GAAGA MspI 90 CCGG C^CGG 264 CCGG PstI 34 CTGCAG CTGCA^G 145 CTGCAG SfcI 34 CTGCAG C^T[GA][CT]AG 145 CTGCAG Tsp509I 129 AATT ^AATT 161 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### AciI AluI BbvI BsaJI Cac8I Fnu4HI HaeIII ItaI MnlI MwoI TseI