################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII AclI AflII AgeI AhdI Alw44I AocI ApaI ApaLI AscI Asp700I AspEI AspI AviII AvrII BamHI BbvCI BcgI BfrI BglI BglII BlnI BlpI Bpu1102I BsaI BsiWI BsmBI Bsp1407I BspDI BspMI BsrGI BssHII BssSI BstAPI BstBI Bsu36I CelII ClaI CpoI DraI DrdI Eam1105I EarI Eco81I EcoRI EcoRV EcoT22I FseI FspI HgaI HpaI Ksp632I KspI MfeI MluI MunI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### Acc65I 110 GGTACC G^GTACC AccIII 180 TCCGGA T^CCGGA AcyI 1027 GGCGCC G[GA]^CG[CT]C AflIII 2180 ACATGT A^C[GA][CT]GT Aor51HI 911 AGCGCT AGC^GCT AseI 2398 ATTAAT AT^TAAT AsnI 2398 ATTAAT AT^TAAT Asp718I 110 GGTACC G^GTACC BbeI 1027 GGCGCC GGCGC^C BbrPI 2002 CACGTG CAC^GTG BsaBI 710 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1027 GGCGCC G[GA]^CG[CT]C BsaWI 180 TCCGGA [AT]^CCGG[AT] BseAI 180 TCCGGA T^CCGGA BspEI 180 TCCGGA T^CCGGA BspHI 1532 TCATGA T^CATGA BspLU11I 2180 ACATGT A^CATGT BsrBI 16 CCGCTC CCGCTC(-3/-3) BsrDI 2543 GCAATG GCAATG(2/0) Bst1107I 1261 GTATAC GTA^TAC BstXI 1871 CCACTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1688 GGATCT [GA]^GATC[CT] BstZ17I 1261 GTATAC GTA^TAC EagI 53 CGGCCG C^GGCCG EclXI 53 CGGCCG C^GGCCG Eco47III 911 AGCGCT AGC^GCT Eco52I 53 CGGCCG C^GGCCG EcoNI 1464 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Hin1I 1027 GGCGCC G[GA]^CG[CT]C HincII 2025 GTTGAC GT[CT]^[GA]AC HindII 2025 GTTGAC GT[CT]^[GA]AC HindIII 570 AAGCTT A^AGCTT KasI 1027 GGCGCC G^GCGCC KpnI 110 GGTACC GGTAC^C MamI 710 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1688 GGATCT [GA]^GATC[CT] MroI 180 TCCGGA T^CCGGA NaeI 55 GCCGGC GCC^GGC NarI 1027 GGCGCC GG^CGCC NcoI 731 CCATGG C^CATGG NdeI 1934 CATATG CA^TATG NgoMIV 55 GCCGGC G^CCGGC NspI 2180 ACATGT [GA]CATG^[CT] PflMI 1930 CCAACATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2002 CACGTG CAC^GTG PmlI 2002 CACGTG CAC^GTG PshBI 2398 ATTAAT AT^TAAT PstI 905 CTGCAG CTGCA^G RcaI 1532 TCATGA T^CATGA ScaI 2752 AGTACT AGT^ACT SexAI 696 ACCAGGT A^CC[AT]GGT SfoI 1027 GGCGCC GGC^GCC SmaI 106 CCCGGG CCC^GGG SmlI 2011 CTCAAG C^T[CT][GA]AG SspI 1055 AATATT AAT^ATT Van91I 1930 CCAACATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1688 GGATCT [GA]^GATC[CT] XmaCI 106 CCCGGG C^CCGGG XmaI 106 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 982 GTAGAC GT^[AC][GT]AC 1261 GTATAC AccII 753 CGCG CG^CG 1676 CGCG AlwNI 237 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG 1362 CAGAAACTG AspHI 31 GTGCTC G[AT]GC[AT]^C 2484 GAGCAC AvaI 106 CCCGGG C^[CT]CG[GA]G 1696 CCCGAG BalI 1880 TGGCCA TGG^CCA 2261 TGGCCA BciVI 974 GGATAC GTATCC(6/5) 2494 GGATAC BclI 203 TGATCA T^GATCA 1583 TGATCA BfaI 461 CTAG C^TAG 631 CTAG BsaAI 256 TACGTG [CT]AC^GT[GA] 2002 CACGTG BsgI 392 GTGCAG GTGCAG(16/14) 2005 GTGCAG BsiEI 53 CGGCCG CG[GA][CT]^CG 624 CGACCG BsiHKAI 31 GTGCTC G[AT]GC[AT]^C 2484 GAGCAC BsmI 514 GAATGC GAATGC(1/-1) 722 GCATTC BsoBI 106 CCCGGG C^[CT]CG[GA]G 1696 CCCGAG BsrFI 55 GCCGGC [GA]^CCGG[CT] 626 ACCGGC BstEII 1392 GGTAACC G^GT[ACGT]ACC 2278 GGTGACC BstPI 1392 GGTAACC G^GT[ACGT]ACC 2278 GGTGACC BstUI 753 CGCG CG^CG 1676 CGCG Cfr10I 55 GCCGGC [GA]^CCGG[CT] 626 ACCGGC DraIII 386 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 458 CACCTAGTG DsaI 731 CCATGG C^C[GA][CT]GG 1895 CCACGG EcoO65I 1392 GGTAACC G^GT[ACGT]ACC 2278 GGTGACC FauI 917 CCCGC CCCGC(4/6) 1423 CCCGC FbaI 203 TGATCA T^GATCA 1583 TGATCA MaeI 461 CTAG C^TAG 631 CTAG MluNI 1880 TGGCCA TGG^CCA 2261 TGGCCA MscI 1880 TGGCCA TGG^CCA 2261 TGGCCA MspA1I 1766 CAGCTG C[AC]G^C[GT]G 2248 CAGCTG MvnI 753 CGCG CG^CG 1676 CGCG PvuII 1766 CAGCTG CAG^CTG 2248 CAGCTG SfaNI 1371 GATGC GCATC(5/9) 1490 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI