Restriction map for
pF1KE1772
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaBI BsaI BsaWI BseAI BsgI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HinfI HpaI HphI KpnI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
NcoI NdeI NheI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tru9I Tsp509I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AlwNI 484 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 433 CCTCAGG CC^T[ACGT]AGG
BfaI 616 CTAG C^TAG
BglI 186 GCCGTGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 680 TACGTG [CT]AC^GT[GA]
BsiEI 354 CGGCCG CG[GA][CT]^CG
BsmBI 145 GAGACG CGTCTC(1/5)
BsrBI 703 CCGCTC CCGCTC(-3/-3)
BsrI 808 ACTGG ACTGG(1/-1)
BssHII 413 GCGCGC G^CGCGC
BstAPI 612 GCAGCTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 433 CCTCAGG CC^T[ACGT]AGG
DpnII 138 GATC ^GATC
DsaI 351 CCGCGG C^C[GA][CT]GG
EagI 354 CGGCCG C^GGCCG
EclXI 354 CGGCCG C^GGCCG
Eco52I 354 CGGCCG C^GGCCG
Eco81I 433 CCTCAGG CC^T[ACGT]AGG
HindIII 688 AAGCTT A^AGCTT
KspI 351 CCGCGG CCGC^GG
MaeI 616 CTAG C^TAG
MaeII 681 ACGT A^CGT
MboI 138 GATC ^GATC
NdeII 138 GATC ^GATC
NlaIII 733 CATG CATG^
NotI 353 GCGGCCGC GC^GGCCGC
NruI 664 TCGCGA TCG^CGA
PvuII 154 CAGCTG CAG^CTG
SacI 394 GAGCTC GAGCT^C
SacII 351 CCGCGG CCGC^GG
Sau3AI 138 GATC ^GATC
SfaNI 476 GCATC GCATC(5/9)
Tsp45I 773 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AspHI 394 GAGCTC G[AT]GC[AT]^C
619 GTGCTC
BsiHKAI 394 GAGCTC G[AT]GC[AT]^C
619 GTGCTC
BsmAI 145 GAGAC GTCTC(1/5)
301 GAGAC
BstF5I 477 CATCC GGATG(2/0)
511 GGATG
DdeI 434 CTCAG C^T[ACGT]AG
802 CTCAG
EaeI 354 CGGCCG [CT]^GGCC[GA]
389 TGGCCG
EarI 22 GAAGAG CTCTTC(1/4)
55 GAAGAG
EcoNI 633 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
785 CCTCCGGGAGG
FokI 477 CATCC GGATG(9/13)
511 GGATG
Ksp632I 22 GAAGAG CTCTTC(1/4)
55 GAAGAG
MaeIII 678 GTTAC ^GT[ACGT]AC
773 GTGAC
MboII 22 GAAGA GAAGA(8/7)
55 GAAGA
PshAI 554 GACGCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
713 GACCTCTGTC
SmaI 198 CCCGGG CCC^GGG
219 CCCGGG
XmaCI 198 CCCGGG C^CCGGG
219 CCCGGG
XmaI 198 CCCGGG C^CCGGG
219 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BmyI BsaHI BsaJI
BseRI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrFI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DraII
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII ItaI KasI MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NgoMIV NlaIV
PpuMI PstI Sau96I ScrFI SfcI
SfoI TseI TspRI