################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII AccI AclI AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AvrII BbeI BbvCI BfrI BlnI BlpI Bpu1102I BsaBI BsiWI BsmBI Bsp1407I BsrBI BsrGI BssHII Bst1107I BstBI BstZ17I CelII CpoI DraI EagI EclXI Eco47III Eco52I EcoT22I FseI HincII HindII HindIII HpaI KasI MamI MfeI MluI MunI NarI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshBI Psp1406I RsrII SalI SbfI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 2371 GGTACC G^GTACC AcyI 789 GGCGTC G[GA]^CG[CT]C AhdI 3598 GACCTCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 2371 GGTACC G^GTACC AspEI 3598 GACCTCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 3776 TGCGCA TGC^GCA BalI 3030 TGGCCA TGG^CCA BamHI 3560 GGATCC G^GATCC BbrPI 2591 CACGTG CAC^GTG BcgI 4013 GCACTGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1772 GTATCC GTATCC(6/5) BclI 3749 TGATCA T^GATCA BsaHI 789 GGCGTC G[GA]^CG[CT]C BsiEI 505 CGATCG CG[GA][CT]^CG BspDI 2442 ATCGAT AT^CGAT BspHI 1085 TCATGA T^CATGA BspLU11I 3410 ACATGT A^CATGT BstXI 4290 CCAAACTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 2442 ATCGAT AT^CGAT Eam1105I 3598 GACCTCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 1267 GATATC GAT^ATC FbaI 3749 TGATCA T^GATCA FspI 3776 TGCGCA TGC^GCA Hin1I 789 GGCGTC G[GA]^CG[CT]C KpnI 2371 GGTACC GGTAC^C MluNI 3030 TGGCCA TGG^CCA MscI 3030 TGGCCA TGG^CCA NdeI 911 CATATG CA^TATG PmaCI 2591 CACGTG CAC^GTG PmlI 2591 CACGTG CAC^GTG PvuI 505 CGATCG CGAT^CG RcaI 1085 TCATGA T^CATGA ScaI 3305 AGTACT AGT^ACT ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflIII 2969 ACGTGT A^C[GA][CT]GT 3410 ACATGT AocI 2356 CCTCAGG CC^T[ACGT]AGG 2816 CCTCAGG BglII 3082 AGATCT A^GATCT 4107 AGATCT BsaAI 2591 CACGTG [CT]AC^GT[GA] 2968 TACGTG BsmI 2924 GCATTC GAATGC(1/-1) 3398 GCATTC BspMI 255 ACCTGC ACCTGC(4/8) 1056 ACCTGC BstAPI 3706 GCAACCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3967 GCAGGCGGTGC BstEII 2128 GGTGACC G^GT[ACGT]ACC 4438 GGTGACC BstPI 2128 GGTGACC G^GT[ACGT]ACC 4438 GGTGACC Bsu36I 2356 CCTCAGG CC^T[ACGT]AGG 2816 CCTCAGG DraIII 1970 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG 3187 CACGTTGTG EaeI 2379 TGGCCG [CT]^GGCC[GA] 3030 TGGCCA Eco81I 2356 CCTCAGG CC^T[ACGT]AGG 2816 CCTCAGG EcoNI 290 CCTTTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2938 CCTTCTGAAGG EcoO65I 2128 GGTGACC G^GT[ACGT]ACC 4438 GGTGACC EcoRI 531 GAATTC G^AATTC 3514 GAATTC HaeII 1327 GGCGCT [GA]GCGC^[CT] 1353 AGCGCC KspI 677 CCGCGG CCGC^GG 1586 CCGCGG MseI 482 TTAA T^TAA 2558 TTAA PflMI 455 CCACCCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1572 CCAGGGCATGG SacII 677 CCGCGG CCGC^GG 1586 CCGCGG SapI 1443 GCTCTTC GCTCTTC(1/4) 3923 GCTCTTC SphI 845 GCATGC GCATG^C 4148 GCATGC TfiI 2291 GAATC G^A[AT]TC 3039 GATTC Tru9I 482 TTAA T^TAA 2558 TTAA Van91I 455 CCACCCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1572 CCAGGGCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 127 enzymes) # ######################################### AccII AccIII AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AspI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BsrDI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MroI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PaeR7I PflFI PleI PpuMI PshAI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SfiI SmaI SmlI StuI StyI TaqI TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoI XhoII XmaCI XmaI