Restriction map for
pF1KE2406
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# NO CUT ENZYMES ( 72 enzymes) #
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AatII AccI AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I AvrII BbeI BbvCI
BfrI BlnI BlpI Bpu1102I BsaBI
BsiWI BsmBI Bsp1407I BsrBI BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
CpoI DraI EagI EclXI Eco47III
Eco52I EcoT22I FseI HincII HindII
HindIII HpaI KasI MamI MfeI
MluI MunI NarI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I RsrII SalI
SbfI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
XbaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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Acc65I 2371 GGTACC G^GTACC
AcyI 789 GGCGTC G[GA]^CG[CT]C
AhdI 3598 GACCTCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 2371 GGTACC G^GTACC
AspEI 3598 GACCTCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3776 TGCGCA TGC^GCA
BalI 3030 TGGCCA TGG^CCA
BamHI 3560 GGATCC G^GATCC
BbrPI 2591 CACGTG CAC^GTG
BcgI 4013 GCACTGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1772 GTATCC GTATCC(6/5)
BclI 3749 TGATCA T^GATCA
BsaHI 789 GGCGTC G[GA]^CG[CT]C
BsiEI 505 CGATCG CG[GA][CT]^CG
BspDI 2442 ATCGAT AT^CGAT
BspHI 1085 TCATGA T^CATGA
BspLU11I 3410 ACATGT A^CATGT
BstXI 4290 CCAAACTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 2442 ATCGAT AT^CGAT
Eam1105I 3598 GACCTCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1267 GATATC GAT^ATC
FbaI 3749 TGATCA T^GATCA
FspI 3776 TGCGCA TGC^GCA
Hin1I 789 GGCGTC G[GA]^CG[CT]C
KpnI 2371 GGTACC GGTAC^C
MluNI 3030 TGGCCA TGG^CCA
MscI 3030 TGGCCA TGG^CCA
NdeI 911 CATATG CA^TATG
PmaCI 2591 CACGTG CAC^GTG
PmlI 2591 CACGTG CAC^GTG
PvuI 505 CGATCG CGAT^CG
RcaI 1085 TCATGA T^CATGA
ScaI 3305 AGTACT AGT^ACT
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# TWO CUT ENZYMES ( 26 enzymes) #
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AflIII 2969 ACGTGT A^C[GA][CT]GT
3410 ACATGT
AocI 2356 CCTCAGG CC^T[ACGT]AGG
2816 CCTCAGG
BglII 3082 AGATCT A^GATCT
4107 AGATCT
BsaAI 2591 CACGTG [CT]AC^GT[GA]
2968 TACGTG
BsmI 2924 GCATTC GAATGC(1/-1)
3398 GCATTC
BspMI 255 ACCTGC ACCTGC(4/8)
1056 ACCTGC
BstAPI 3706 GCAACCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3967 GCAGGCGGTGC
BstEII 2128 GGTGACC G^GT[ACGT]ACC
4438 GGTGACC
BstPI 2128 GGTGACC G^GT[ACGT]ACC
4438 GGTGACC
Bsu36I 2356 CCTCAGG CC^T[ACGT]AGG
2816 CCTCAGG
DraIII 1970 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3187 CACGTTGTG
EaeI 2379 TGGCCG [CT]^GGCC[GA]
3030 TGGCCA
Eco81I 2356 CCTCAGG CC^T[ACGT]AGG
2816 CCTCAGG
EcoNI 290 CCTTTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2938 CCTTCTGAAGG
EcoO65I 2128 GGTGACC G^GT[ACGT]ACC
4438 GGTGACC
EcoRI 531 GAATTC G^AATTC
3514 GAATTC
HaeII 1327 GGCGCT [GA]GCGC^[CT]
1353 AGCGCC
KspI 677 CCGCGG CCGC^GG
1586 CCGCGG
MseI 482 TTAA T^TAA
2558 TTAA
PflMI 455 CCACCCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1572 CCAGGGCATGG
SacII 677 CCGCGG CCGC^GG
1586 CCGCGG
SapI 1443 GCTCTTC GCTCTTC(1/4)
3923 GCTCTTC
SphI 845 GCATGC GCATG^C
4148 GCATGC
TfiI 2291 GAATC G^A[AT]TC
3039 GATTC
Tru9I 482 TTAA T^TAA
2558 TTAA
Van91I 455 CCACCCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1572 CCAGGGCATGG
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# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AccIII AciI AcsI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AspI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspEI BsrDI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MroI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PaeR7I PflFI PleI PpuMI PshAI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfiI SmaI SmlI StuI StyI
TaqI TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XcmI XhoI XhoII
XmaCI XmaI