Restriction map for
pF1KE2062
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccII AflII AgeI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BamHI
BfrI BlnI BlpI Bpu1102I BsaBI
BsiWI BsmI BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BssHII BssSI
BstBI BstUI CelII ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoNI EcoT22I FseI
FspI HindIII KpnI KspI MamI
MfeI MluI MunI MvnI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI PstI RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfuI SnaBI SpeI Sse8387I
SspI StuI SwaI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccIII 388 TCCGGA T^CCGGA
AclI 2244 AACGTT AA^CGTT
AcsI 2276 GAATTC [GA]^AATT[CT]
AcyI 286 GGCGCC G[GA]^CG[CT]C
AflIII 725 ACACGT A^C[GA][CT]GT
AhdI 1804 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1082 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1780 CCTGAGG CC^T[ACGT]AGG
ApoI 2276 GAATTC [GA]^AATT[CT]
AspEI 1804 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1744 CCCGGG C^[CT]CG[GA]G
BalI 1829 TGGCCA TGG^CCA
BanI 286 GGCGCC G^G[CT][GA]CC
BbeI 286 GGCGCC GGCGC^C
BbrPI 877 CACGTG CAC^GTG
BbvCI 2313 CCTCAGC CCTCAGC(-5/-2)
BcgI 597 GCAGGAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 206 TGATCA T^GATCA
BglII 2084 AGATCT A^GATCT
BsaHI 286 GGCGCC G[GA]^CG[CT]C
BsaWI 388 TCCGGA [AT]^CCGG[AT]
BseAI 388 TCCGGA T^CCGGA
BsiEI 751 CGATCG CG[GA][CT]^CG
BsmBI 815 GAGACG CGTCTC(1/5)
BsoBI 1744 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2195 TGTACA T^GTACA
BspEI 388 TCCGGA T^CCGGA
BsrGI 2195 TGTACA T^GTACA
Bst1107I 2035 GTATAC GTA^TAC
BstAPI 2220 GCATGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 2084 AGATCT [GA]^GATC[CT]
BstZ17I 2035 GTATAC GTA^TAC
Bsu36I 1780 CCTGAGG CC^T[ACGT]AGG
Eam1105I 1804 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1780 CCTGAGG CC^T[ACGT]AGG
EcoRI 2276 GAATTC G^AATTC
FbaI 206 TGATCA T^GATCA
HgaI 1006 GACGC GACGC(5/10)
Hin1I 286 GGCGCC G[GA]^CG[CT]C
HpaI 949 GTTAAC GTT^AAC
KasI 286 GGCGCC G^GCGCC
MflI 2084 AGATCT [GA]^GATC[CT]
MluNI 1829 TGGCCA TGG^CCA
MroI 388 TCCGGA T^CCGGA
MscI 1829 TGGCCA TGG^CCA
NarI 286 GGCGCC GG^CGCC
NcoI 41 CCATGG C^CATGG
NspI 2220 GCATGC [GA]CATG^[CT]
PmaCI 877 CACGTG CAC^GTG
PmlI 877 CACGTG CAC^GTG
PpuMI 1409 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 2244 AACGTT AA^CGTT
PvuI 751 CGATCG CGAT^CG
SapI 106 GAAGAGC GCTCTTC(1/4)
SfcI 335 CTGTAG C^T[GA][CT]AG
SfoI 286 GGCGCC GGC^GCC
SgrAI 942 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 1744 CCCGGG CCC^GGG
SphI 2220 GCATGC GCATG^C
SspBI 2195 TGTACA T^GTACA
XhoII 2084 AGATCT [GA]^GATC[CT]
XmaCI 1744 CCCGGG C^CCGGG
XmaI 1744 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 337 GTAGAC GT^[AC][GT]AC
2035 GTATAC
Alw44I 229 GTGCAC G^TGCAC
1243 GTGCAC
AlwI 869 GGATC GGATC(4/5)
2271 GGATC
ApaI 238 GGGCCC GGGCC^C
904 GGGCCC
ApaLI 229 GTGCAC G^TGCAC
1243 GTGCAC
BbsI 2263 GAAGAC GAAGAC(2/6)
1153 GTCTTC
BglI 756 GCCTCACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
906 GCCCGACGGGC
BpuAI 2263 GAAGAC GAAGAC(2/6)
1153 GTCTTC
BsaI 2238 GAGACC GGTCTC(1/5)
1386 GGTCTC
BsgI 1659 CTGCAC GTGCAG(16/14)
2181 CTGCAC
BstEII 36 GGTCACC G^GT[ACGT]ACC
1533 GGTCACC
BstPI 36 GGTCACC G^GT[ACGT]ACC
1533 GGTCACC
BstXI 1832 CCAGAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1996 CCAGTCAAGTGG
DraII 237 GGGGCCC [GA]G^G[ACGT]CC[CT]
1409 AGGACCC
EcoO109I 237 GGGGCCC [GA]G^G[ACGT]CC[CT]
1409 AGGACCC
EcoO65I 36 GGTCACC G^GT[ACGT]ACC
1533 GGTCACC
EcoRV 1047 GATATC GAT^ATC
1948 GATATC
EcoT14I 41 CCATGG C^C[AT][AT]GG
1523 CCAAGG
FauI 1326 CCCGC CCCGC(4/6)
1030 GCGGG
HaeII 286 GGCGCC [GA]GCGC^[CT]
1083 AGCGCC
HincII 949 GTTAAC GT[CT]^[GA]AC
1068 GTTGAC
HindII 949 GTTAAC GT[CT]^[GA]AC
1068 GTTGAC
NaeI 786 GCCGGC GCC^GGC
1236 GCCGGC
NgoMIV 786 GCCGGC G^CCGGC
1236 GCCGGC
PflMI 563 CCATGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1081 CCAGCGCCTGG
PvuII 6 CAGCTG CAG^CTG
1825 CAGCTG
SmlI 925 CTCAAG C^T[CT][GA]AG
934 CTCAAG
StyI 41 CCATGG C^C[AT][AT]GG
1523 CCAAGG
TfiI 2118 GATTC G^A[AT]TC
2383 GATTC
Van91I 563 CCATGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1081 CCAGCGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanII BbvI BciVI BcnI BfaI
BmyI BpmI BsaAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI