################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI AlwI AocI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaBI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI Bst1107I BstBI BstEII BstF5I BstPI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FspI HindIII HpaI KpnI MaeI MamI MfeI MflI MluI MroI MseI MunI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp509I TthHB8I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 343 GACGTC GACGT^C AfaI 508 GTAC GT^AC Aor51HI 659 AGCGCT AGC^GCT AspI 940 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 809 CCCGGG C^[CT]CG[GA]G BanII 309 GAGCCC G[GA]GC[CT]^C BbrPI 474 CACGTG CAC^GTG BlpI 306 GCTGAGC GC^T[ACGT]AGC BpmI 559 CTGGAG CTGGAG(16/14) Bpu1102I 306 GCTGAGC GC^T[ACGT]AGC BsgI 688 CTGCAC GTGCAG(16/14) BsmFI 815 GTCCC GGGAC(10/14) BsoBI 809 CCCGGG C^[CT]CG[GA]G BsrI 635 ACTGG ACTGG(1/-1) BssSI 160 CTCGTG CACGAG(-5/-1) BstAPI 453 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 383 CCATCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 306 GCTGAGC GC^T[ACGT]AGC DdeI 307 CTGAG C^T[ACGT]AG DpnII 1008 GATC ^GATC DrdI 571 GACTACGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 268 CTCTTC CTCTTC(1/4) Eco47III 659 AGCGCT AGC^GCT EcoT14I 338 CCATGG C^C[AT][AT]GG FseI 50 GGCCGGCC GGCCGG^CC HincII 1171 GTCAAC GT[CT]^[GA]AC HindII 1171 GTCAAC GT[CT]^[GA]AC HinfI 972 GACTC G^A[ACGT]TC Ksp632I 268 CTCTTC CTCTTC(1/4) MaeIII 1153 GTCAC ^GT[ACGT]AC MboI 1008 GATC ^GATC NcoI 338 CCATGG C^CATGG NdeII 1008 GATC ^GATC PflFI 940 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 972 GACTC GAGTC(4/5) PmaCI 474 CACGTG CAC^GTG PmlI 474 CACGTG CAC^GTG PvuII 454 CAGCTG CAG^CTG RsaI 508 GTAC GT^AC SapI 267 GCTCTTC GCTCTTC(1/4) Sau3AI 1008 GATC ^GATC SmaI 809 CCCGGG CCC^GGG StyI 338 CCATGG C^C[AT][AT]GG Tsp45I 1153 GTCAC ^GT[GC]AC TspRI 1200 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 940 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 858 CCAGGACCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 809 CCCGGG C^CCGGG XmaI 809 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 274 GTCTAC GT^[AC][GT]AC 580 GTCTAC AluI 455 AGCT AG^CT 686 AGCT Alw44I 354 GTGCAC G^TGCAC 1093 GTGCAC AlwNI 454 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 1105 CAGGCGCTG ApaLI 354 GTGCAC G^TGCAC 1093 GTGCAC BalI 176 TGGCCA TGG^CCA 335 TGGCCA BanI 469 GGCGCC G^G[CT][GA]CC 501 GGCGCC BbeI 469 GGCGCC GGCGC^C 501 GGCGCC BbsI 1204 GTCTTC GAAGAC(2/6) 1228 GTCTTC BpuAI 1204 GTCTTC GAAGAC(2/6) 1228 GTCTTC BsaAI 474 CACGTG [CT]AC^GT[GA] 574 TACGTG BseRI 241 CTCCTC GAGGAG(10/8) 244 CTCCTC BsrBI 265 CCGCTC CCGCTC(-3/-3) 616 CCGCTC BssHII 186 GCGCGC G^CGCGC 698 GCGCGC EcoNI 756 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 852 CCTCCCCCAGG HphI 1010 TCACC GGTGA(8/7) 1154 TCACC KasI 469 GGCGCC G^GCGCC 501 GGCGCC MluNI 176 TGGCCA TGG^CCA 335 TGGCCA MscI 176 TGGCCA TGG^CCA 335 TGGCCA MslI 333 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 384 CATCAGCGTG NaeI 51 GCCGGC GCC^GGC 449 GCCGGC NarI 469 GGCGCC GG^CGCC 501 GGCGCC NgoMIV 51 GCCGGC G^CCGGC 449 GCCGGC NotI 229 GCGGCCGC GC^GGCCGC 533 GCGGCCGC PstI 193 CTGCAG CTGCA^G 655 CTGCAG SfaNI 64 GCATC GCATC(5/9) 76 GCATC SfcI 193 CTGCAG C^T[GA][CT]AG 655 CTGCAG SfoI 469 GGCGCC GGC^GCC 501 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AccII AciI AcyI AspHI AvaII BbvI BcnI BglI BmyI BsaHI BsaJI BsiEI BsiHKAI BsiYI BslI Bsp1286I BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI KspI MaeII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PpuMI SacII Sau96I ScrFI TseI