Restriction map for
pF1KE2200
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII ClaI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoRI
EcoRV FbaI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
MaeI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NspV PacI PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI RcaI SalI SapI SbfI
SexAI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AlwNI 463 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 145 AGCGCT AGC^GCT
ApaI 532 GGGCCC GGGCC^C
AspHI 963 GAGCTC G[AT]GC[AT]^C
BalI 1456 TGGCCA TGG^CCA
BamHI 741 GGATCC G^GATCC
BbrPI 18 CACGTG CAC^GTG
BbvCI 852 GCTGAGG CCTCAGC(-5/-2)
BsaAI 18 CACGTG [CT]AC^GT[GA]
BsaI 1150 GAGACC GGTCTC(1/5)
BsgI 828 CTGCAC GTGCAG(16/14)
BsiEI 1138 CGGTCG CG[GA][CT]^CG
BsiHKAI 963 GAGCTC G[AT]GC[AT]^C
BspMI 602 ACCTGC ACCTGC(4/8)
BssSI 454 CACGAG CACGAG(-5/-1)
BstEII 657 GGTCACC G^GT[ACGT]ACC
BstPI 657 GGTCACC G^GT[ACGT]ACC
BstXI 498 CCAGTTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 741 GGATCC [GA]^GATC[CT]
CpoI 1384 CGGTCCG CG^G[AT]CCG
DpnII 742 GATC ^GATC
Eco47III 145 AGCGCT AGC^GCT
EcoO65I 657 GGTCACC G^GT[ACGT]ACC
EcoT14I 746 CCATGG C^C[AT][AT]GG
KspI 174 CCGCGG CCGC^GG
MboI 742 GATC ^GATC
MflI 741 GGATCC [GA]^GATC[CT]
MluNI 1456 TGGCCA TGG^CCA
MscI 1456 TGGCCA TGG^CCA
NaeI 33 GCCGGC GCC^GGC
NcoI 746 CCATGG C^CATGG
NdeII 742 GATC ^GATC
NgoMIV 33 GCCGGC G^CCGGC
NspI 1511 ACATGC [GA]CATG^[CT]
PaeR7I 616 CTCGAG C^TCGAG
PmaCI 18 CACGTG CAC^GTG
PmlI 18 CACGTG CAC^GTG
RsrII 1384 CGGTCCG CG^G[AT]CCG
SacI 963 GAGCTC GAGCT^C
SacII 174 CCGCGG CCGC^GG
Sau3AI 742 GATC ^GATC
ScaI 779 AGTACT AGT^ACT
SgrAI 308 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 616 CTCGAG C^T[CT][GA]AG
StyI 746 CCATGG C^C[AT][AT]GG
TfiI 918 GAATC G^A[AT]TC
XcmI 1532 CCAAACTCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 616 CTCGAG C^TCGAG
XhoII 741 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 19 ACGTGT A^C[GA][CT]GT
1107 ACGTGT
AlwI 742 GATCC GGATC(4/5)
741 GGATC
Asp700I 1289 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1344 GAAACCATTC
BbeI 130 GGCGCC GGCGC^C
306 GGCGCC
BbsI 1398 GAAGAC GAAGAC(2/6)
1081 GTCTTC
BglI 133 GCCGCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
752 GCCAGCAGGGC
BpuAI 1398 GAAGAC GAAGAC(2/6)
1081 GTCTTC
BsmBI 38 CGTCTC CGTCTC(1/5)
154 CGTCTC
BsmI 904 GAATGC GAATGC(1/-1)
1091 GCATTC
BsrBI 54 CCGCTC CCGCTC(-3/-3)
288 GAGCGG
DsaI 174 CCGCGG C^C[GA][CT]GG
746 CCATGG
EcoT22I 906 ATGCAT ATGCA^T
1513 ATGCAT
KasI 130 GGCGCC G^GCGCC
306 GGCGCC
MboII 1398 GAAGA GAAGA(8/7)
1082 TCTTC
MseI 943 TTAA T^TAA
1208 TTAA
NarI 130 GGCGCC GG^CGCC
306 GGCGCC
NsiI 906 ATGCAT ATGCA^T
1513 ATGCAT
SfcI 73 CTACAG C^T[GA][CT]AG
864 CTACAG
SfoI 130 GGCGCC GGC^GCC
306 GGCGCC
SmaI 105 CCCGGG CCC^GGG
330 CCCGGG
TaqI 617 TCGA T^CGA
665 TCGA
Tru9I 943 TTAA T^TAA
1208 TTAA
Tsp509I 877 AATT ^AATT
1493 AATT
TthHB8I 617 TCGA T^CGA
665 TCGA
XmaCI 105 CCCGGG C^CCGGG
330 CCCGGG
XmaI 105 CCCGGG C^CCGGG
330 CCCGGG
XmnI 1289 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1344 GAAACCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaHI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PleI PpuMI
PvuII RsaI Sau96I ScrFI SfaNI
TseI Tsp45I TspRI