Restriction map for
pF1KE3532
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII BalI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MseI MunI NdeI NheI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Alw44I 889 GTGCAC G^TGCAC
AlwNI 904 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 889 GTGCAC G^TGCAC
AvrII 1132 CCTAGG C^CTAGG
BamHI 1173 GGATCC G^GATCC
BlnI 1132 CCTAGG C^CTAGG
BpmI 622 CTGGAG CTGGAG(16/14)
BseRI 1198 CTCCTC GAGGAG(10/8)
BsmBI 163 CGTCTC CGTCTC(1/5)
BssHII 557 GCGCGC G^CGCGC
BstAPI 1025 GCACCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1173 GGATCC [GA]^GATC[CT]
DraIII 1026 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 671 CCATGG C^C[GA][CT]GG
HinfI 538 GACTC G^A[ACGT]TC
MflI 1173 GGATCC [GA]^GATC[CT]
MslI 1075 CACAGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 80 CAGCTG C[AC]G^C[GT]G
NcoI 671 CCATGG C^CATGG
NlaIII 672 CATG CATG^
NotI 652 GCGGCCGC GC^GGCCGC
PleI 538 GACTC GAGTC(4/5)
PpuMI 905 AGGACCT [GA]G^G[AT]CC[CT]
PstI 313 CTGCAG CTGCA^G
PvuII 80 CAGCTG CAG^CTG
SapI 108 GCTCTTC GCTCTTC(1/4)
SfaNI 614 GCATC GCATC(5/9)
SfcI 313 CTGCAG C^T[GA][CT]AG
TaqI 752 TCGA T^CGA
Tsp45I 64 GTGAC ^GT[GC]AC
TthHB8I 752 TCGA T^CGA
XhoII 1173 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 218 GTAC GT^AC
765 GTAC
ApaI 271 GGGCCC GGGCC^C
506 GGGCCC
BanII 271 GGGCCC G[GA]GC[CT]^C
506 GGGCCC
BfaI 611 CTAG C^TAG
1133 CTAG
BsiEI 385 CGGCCG CG[GA][CT]^CG
653 CGGCCG
BspMI 57 ACCTGC ACCTGC(4/8)
936 GCAGGT
BstF5I 834 CATCC GGATG(2/0)
924 CATCC
DdeI 166 CTCAG C^T[ACGT]AG
910 CTGAG
DpnII 1084 GATC ^GATC
1174 GATC
EagI 385 CGGCCG C^GGCCG
653 CGGCCG
EclXI 385 CGGCCG C^GGCCG
653 CGGCCG
Eco52I 385 CGGCCG C^GGCCG
653 CGGCCG
FauI 254 CCCGC CCCGC(4/6)
597 GCGGG
FokI 834 CATCC GGATG(9/13)
924 CATCC
MaeI 611 CTAG C^TAG
1133 CTAG
MaeII 461 ACGT A^CGT
1080 ACGT
MaeIII 8 GTAAC ^GT[ACGT]AC
64 GTGAC
MboI 1084 GATC ^GATC
1174 GATC
NaeI 737 GCCGGC GCC^GGC
1206 GCCGGC
NdeII 1084 GATC ^GATC
1174 GATC
NgoMIV 737 GCCGGC G^CCGGC
1206 GCCGGC
RsaI 218 GTAC GT^AC
765 GTAC
Sau3AI 1084 GATC ^GATC
1174 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AspHI AvaII BanI BbeI BbvI
BcnI BglI BmyI BsaHI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BsrI BssKI BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DraII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI KasI Ksp632I
MboII MnlI MspI MvaI MvnI
MwoI NarI NciI NlaIV Sau96I
ScrFI SexAI SfoI StyI TseI
TspRI XcmI