################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspHI BspLU11I BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I StuI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AhdI 226 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 667 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 226 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 270 GTGCTC G[AT]GC[AT]^C AspI 442 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 877 CCCGAG C^[CT]CG[GA]G BanI 1208 GGCACC G^G[CT][GA]CC BcgI 372 CGATTTGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 1468 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1329 AGATCT A^GATCT BpmI 582 CTGGAG CTGGAG(16/14) BsgI 333 CTGCAC GTGCAG(16/14) BsiHKAI 270 GTGCTC G[AT]GC[AT]^C BsmBI 342 GAGACG CGTCTC(1/5) BsmI 100 GAATGC GAATGC(1/-1) BsoBI 877 CCCGAG C^[CT]CG[GA]G Bsp1407I 1295 TGTACA T^GTACA BspMI 262 ACCTGC ACCTGC(4/8) BsrBI 117 GAGCGG CCGCTC(-3/-3) BsrDI 866 GCAATG GCAATG(2/0) BsrGI 1295 TGTACA T^GTACA DrdI 520 GACCTCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 226 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HincII 1177 GTTGAC GT[CT]^[GA]AC HindII 1177 GTTGAC GT[CT]^[GA]AC NcoI 992 CCATGG C^CATGG NspI 1082 GCATGC [GA]CATG^[CT] PflFI 442 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1048 CCACTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 124 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 944 CAGCTG CAG^CTG SphI 1082 GCATGC GCATG^C SspBI 1295 TGTACA T^GTACA SspI 511 AATATT AAT^ATT TaqI 371 TCGA T^CGA Tth111I 442 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 371 TCGA T^CGA Van91I 1048 CCACTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 397 AAATTT [GA]^AATT[CT] 955 GAATTT ApoI 397 AAATTT [GA]^AATT[CT] 955 GAATTT BcnI 228 CCGGG CC^[GC]GG 1263 CCGGG BfaI 62 CTAG C^TAG 1043 CTAG BmyI 270 GTGCTC G[AGT]GC[ACT]^C 380 GTGCCC Bsp1286I 270 GTGCTC G[AGT]GC[ACT]^C 380 GTGCCC BstXI 267 CCAGTGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1325 CCAAAGATCTGG DsaI 112 CCGTGG C^C[GA][CT]GG 992 CCATGG EcoT22I 535 ATGCAT ATGCA^T 1169 ATGCAT FauI 119 GCGGG CCCGC(4/6) 714 GCGGG HapII 228 CCGG C^CGG 1263 CCGG HpaII 228 CCGG C^CGG 1263 CCGG MaeI 62 CTAG C^TAG 1043 CTAG MaeIII 731 GTGAC ^GT[ACGT]AC 1502 GTCAC MseI 500 TTAA T^TAA 1391 TTAA MspA1I 47 CCGCTG C[AC]G^C[GT]G 944 CAGCTG MspI 228 CCGG C^CGG 1263 CCGG NciI 228 CCGGG CC^[GC]GG 1263 CCGGG NsiI 535 ATGCAT ATGCA^T 1169 ATGCAT SfcI 825 CTGTAG C^T[GA][CT]AG 1344 CTATAG Tru9I 500 TTAA T^TAA 1391 TTAA Tsp45I 731 GTGAC ^GT[GC]AC 1502 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BalI BbvI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MboI MboII MflI MluNI MnlI MscI MslI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TfiI TseI Tsp509I TspRI XcmI XhoII