################################## # NO CUT ENZYMES ( 217 enzymes) # ################################## AatII Acc65I AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvaI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TseI Tsp45I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AccI 250 GTCTAC GT^[AC][GT]AC AccII 294 CGCG CG^CG AcsI 130 AAATTT [GA]^AATT[CT] ApoI 130 AAATTT [GA]^AATT[CT] BbsI 208 GTCTTC GAAGAC(2/6) BcgI 36 CGAGAAATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 310 AGATCT A^GATCT BpmI 239 CTGGAG CTGGAG(16/14) BpuAI 208 GTCTTC GAAGAC(2/6) BsrDI 232 GCAATG GCAATG(2/0) BstUI 294 CGCG CG^CG BstXI 107 CCACCCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 310 AGATCT [GA]^GATC[CT] DdeI 404 CTAAG C^T[ACGT]AG HinfI 465 GATTC G^A[ACGT]TC MaeIII 386 GTTAC ^GT[ACGT]AC MflI 310 AGATCT [GA]^GATC[CT] MnlI 96 GAGG CCTC(7/6) MseI 494 TTAA T^TAA MvnI 294 CGCG CG^CG PflMI 356 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG TaqI 35 TCGA T^CGA TfiI 465 GATTC G^A[AT]TC Tru9I 494 TTAA T^TAA TspRI 189 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 35 TCGA T^CGA Van91I 356 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 104 CCACCACCCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 310 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### BsmFI 193 GGGAC GGGAC(10/14) 182 GTCCC BsrI 362 ACTGG ACTGG(1/-1) 389 ACTGG DpnII 225 GATC ^GATC 311 GATC HphI 462 GGTGA GGTGA(8/7) 486 GGTGA MboI 225 GATC ^GATC 311 GATC MboII 14 TCTTC GAAGA(8/7) 209 TCTTC NdeII 225 GATC ^GATC 311 GATC Sau3AI 225 GATC ^GATC 311 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### BsiYI BslI NlaIII Tsp509I