################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI MaeI MamI MfeI MluI MroI MseI MslI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 802 GGTACC G^GTACC AcsI 589 AAATTT [GA]^AATT[CT] AcyI 631 GGCGCC G[GA]^CG[CT]C ApaI 191 GGGCCC GGGCC^C ApoI 589 AAATTT [GA]^AATT[CT] Asp718I 802 GGTACC G^GTACC AvaI 468 CCCGGG C^[CT]CG[GA]G BamHI 524 GGATCC G^GATCC BbeI 631 GGCGCC GGCGC^C BglI 224 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 631 GGCGCC G[GA]^CG[CT]C BsgI 775 CTGCAC GTGCAG(16/14) BsmAI 241 GTCTC GTCTC(1/5) BsmBI 240 CGTCTC CGTCTC(1/5) BsmFI 341 GGGAC GGGAC(10/14) BsoBI 468 CCCGGG C^[CT]CG[GA]G BsrFI 535 ACCGGC [GA]^CCGG[CT] BstXI 513 CCACCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 524 GGATCC [GA]^GATC[CT] Cfr10I 535 ACCGGC [GA]^CCGG[CT] DdeI 361 CTCAG C^T[ACGT]AG Hin1I 631 GGCGCC G[GA]^CG[CT]C HinfI 549 GAATC G^A[ACGT]TC HphI 390 GGTGA GGTGA(8/7) KasI 631 GGCGCC G^GCGCC KpnI 802 GGTACC GGTAC^C MflI 524 GGATCC [GA]^GATC[CT] NarI 631 GGCGCC GG^CGCC NcoI 134 CCATGG C^CATGG PflMI 276 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 341 GGGACCT [GA]G^G[AT]CC[CT] PstI 415 CTGCAG CTGCA^G SapI 402 GCTCTTC GCTCTTC(1/4) SfaNI 431 GCATC GCATC(5/9) SfoI 631 GGCGCC GGC^GCC SmaI 468 CCCGGG CCC^GGG TfiI 549 GAATC G^A[AT]TC Tsp509I 590 AATT ^AATT Van91I 276 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 524 GGATCC [GA]^GATC[CT] XmaCI 468 CCCGGG C^CCGGG XmaI 468 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 166 GTAC GT^AC 803 GTAC AvaII 342 GGACC G^G[AT]CC 619 GGACC BbsI 70 GTCTTC GAAGAC(2/6) 607 GTCTTC BcnI 468 CCCGG CC^[GC]GG 469 CCGGG BpuAI 70 GTCTTC GAAGAC(2/6) 607 GTCTTC BsaAI 265 TACGTG [CT]AC^GT[GA] 661 TACGTG BstAPI 417 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 786 GCAGAGCCTGC BstF5I 333 CATCC GGATG(2/0) 636 CATCC DrdI 343 GACCTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 598 GACCACTGGGTC EarI 64 CTCTTC CTCTTC(1/4) 403 CTCTTC EcoNI 23 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 162 CCTGGTACAGG FokI 333 CATCC GGATG(9/13) 636 CATCC Ksp632I 64 CTCTTC CTCTTC(1/4) 403 CTCTTC KspI 336 CCGCGG CCGC^GG 456 CCGCGG NciI 468 CCCGG CC^[GC]GG 469 CCGGG NlaIII 135 CATG CATG^ 313 CATG RsaI 166 GTAC GT^AC 803 GTAC SacI 7 GAGCTC GAGCT^C 400 GAGCTC SacII 336 CCGCGG CCGC^GG 456 CCGCGG SfcI 124 CTACAG C^T[GA][CT]AG 415 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AccII AciI AluI AlwI AlwNI AspHI BalI BanI BanII BbvI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BsrBI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NdeII NlaIV PvuII Sau3AI Sau96I ScrFI StyI TseI Tsp45I TspRI