Restriction map for
pF1KE2627
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI MaeI MamI MfeI
MluI MroI MseI MslI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tth111I TthHB8I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 802 GGTACC G^GTACC
AcsI 589 AAATTT [GA]^AATT[CT]
AcyI 631 GGCGCC G[GA]^CG[CT]C
ApaI 191 GGGCCC GGGCC^C
ApoI 589 AAATTT [GA]^AATT[CT]
Asp718I 802 GGTACC G^GTACC
AvaI 468 CCCGGG C^[CT]CG[GA]G
BamHI 524 GGATCC G^GATCC
BbeI 631 GGCGCC GGCGC^C
BglI 224 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 631 GGCGCC G[GA]^CG[CT]C
BsgI 775 CTGCAC GTGCAG(16/14)
BsmAI 241 GTCTC GTCTC(1/5)
BsmBI 240 CGTCTC CGTCTC(1/5)
BsmFI 341 GGGAC GGGAC(10/14)
BsoBI 468 CCCGGG C^[CT]CG[GA]G
BsrFI 535 ACCGGC [GA]^CCGG[CT]
BstXI 513 CCACCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 524 GGATCC [GA]^GATC[CT]
Cfr10I 535 ACCGGC [GA]^CCGG[CT]
DdeI 361 CTCAG C^T[ACGT]AG
Hin1I 631 GGCGCC G[GA]^CG[CT]C
HinfI 549 GAATC G^A[ACGT]TC
HphI 390 GGTGA GGTGA(8/7)
KasI 631 GGCGCC G^GCGCC
KpnI 802 GGTACC GGTAC^C
MflI 524 GGATCC [GA]^GATC[CT]
NarI 631 GGCGCC GG^CGCC
NcoI 134 CCATGG C^CATGG
PflMI 276 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 341 GGGACCT [GA]G^G[AT]CC[CT]
PstI 415 CTGCAG CTGCA^G
SapI 402 GCTCTTC GCTCTTC(1/4)
SfaNI 431 GCATC GCATC(5/9)
SfoI 631 GGCGCC GGC^GCC
SmaI 468 CCCGGG CCC^GGG
TfiI 549 GAATC G^A[AT]TC
Tsp509I 590 AATT ^AATT
Van91I 276 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 524 GGATCC [GA]^GATC[CT]
XmaCI 468 CCCGGG C^CCGGG
XmaI 468 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 166 GTAC GT^AC
803 GTAC
AvaII 342 GGACC G^G[AT]CC
619 GGACC
BbsI 70 GTCTTC GAAGAC(2/6)
607 GTCTTC
BcnI 468 CCCGG CC^[GC]GG
469 CCGGG
BpuAI 70 GTCTTC GAAGAC(2/6)
607 GTCTTC
BsaAI 265 TACGTG [CT]AC^GT[GA]
661 TACGTG
BstAPI 417 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
786 GCAGAGCCTGC
BstF5I 333 CATCC GGATG(2/0)
636 CATCC
DrdI 343 GACCTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
598 GACCACTGGGTC
EarI 64 CTCTTC CTCTTC(1/4)
403 CTCTTC
EcoNI 23 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
162 CCTGGTACAGG
FokI 333 CATCC GGATG(9/13)
636 CATCC
Ksp632I 64 CTCTTC CTCTTC(1/4)
403 CTCTTC
KspI 336 CCGCGG CCGC^GG
456 CCGCGG
NciI 468 CCCGG CC^[GC]GG
469 CCGGG
NlaIII 135 CATG CATG^
313 CATG
RsaI 166 GTAC GT^AC
803 GTAC
SacI 7 GAGCTC GAGCT^C
400 GAGCTC
SacII 336 CCGCGG CCGC^GG
456 CCGCGG
SfcI 124 CTACAG C^T[GA][CT]AG
415 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI BalI BanI BanII BbvI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI Bsp1286I BsrBI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI MaeII MaeIII MboI
MboII MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIV PvuII Sau3AI Sau96I ScrFI
StyI TseI Tsp45I TspRI