Restriction map for
pF1KE2610
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaHI BseRI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MfeI MluI MseI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tsp509I Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 26 enzymes) #
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AccIII 404 TCCGGA T^CCGGA
AlwNI 730 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 466 GTGCTC G[AT]GC[AT]^C
AvaII 402 GGTCC G^G[AT]CC
BamHI 560 GGATCC G^GATCC
BanI 751 GGCACC G^G[CT][GA]CC
BsaI 382 GAGACC GGTCTC(1/5)
BsaWI 404 TCCGGA [AT]^CCGG[AT]
BseAI 404 TCCGGA T^CCGGA
BsiHKAI 466 GTGCTC G[AT]GC[AT]^C
BspEI 404 TCCGGA T^CCGGA
BspMI 210 GCAGGT ACCTGC(4/8)
BsrBI 594 CCGCTC CCGCTC(-3/-3)
BstAPI 459 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DdeI 129 CTCAG C^T[ACGT]AG
EcoNI 795 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 75 GGCGCT [GA]GCGC^[CT]
HgaI 255 GACGC GACGC(5/10)
MroI 404 TCCGGA T^CCGGA
NcoI 200 CCATGG C^CATGG
NspI 38 GCATGT [GA]CATG^[CT]
PshAI 490 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 457 CTGCAG CTGCA^G
SexAI 188 ACCTGGT A^CC[AT]GGT
SfaNI 4 GATGC GCATC(5/9)
SfiI 225 GGCCAGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
ApaI 233 GGGCCC GGGCC^C
655 GGGCCC
BalI 95 TGGCCA TGG^CCA
423 TGGCCA
BbsI 475 GTCTTC GAAGAC(2/6)
643 GTCTTC
BpuAI 475 GTCTTC GAAGAC(2/6)
643 GTCTTC
BsaBI 171 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
525 GATTGGGATC
BsgI 48 GTGCAG GTGCAG(16/14)
208 GTGCAG
BsmFI 21 GGGAC GGGAC(10/14)
766 GGGAC
BstEII 192 GGTCACC G^GT[ACGT]ACC
213 GGTCACC
BstF5I 375 CATCC GGATG(2/0)
3 GGATG
BstPI 192 GGTCACC G^GT[ACGT]ACC
213 GGTCACC
BstYI 530 GGATCT [GA]^GATC[CT]
560 GGATCC
EcoO65I 192 GGTCACC G^GT[ACGT]ACC
213 GGTCACC
EcoT14I 200 CCATGG C^C[AT][AT]GG
387 CCTTGG
FokI 375 CATCC GGATG(9/13)
3 GGATG
HinfI 585 GAATC G^A[ACGT]TC
805 GAATC
MaeII 24 ACGT A^CGT
398 ACGT
MamI 171 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
525 GATTGGGATC
MflI 530 GGATCT [GA]^GATC[CT]
560 GGATCC
MluNI 95 TGGCCA TGG^CCA
423 TGGCCA
MscI 95 TGGCCA TGG^CCA
423 TGGCCA
MslI 195 CACCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
201 CATGGCTGTG
PflMI 318 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
426 CCAGGCGGTGG
PvuII 319 CAGCTG CAG^CTG
460 CAGCTG
SfcI 166 CTACAG C^T[GA][CT]AG
457 CTGCAG
StyI 200 CCATGG C^C[AT][AT]GG
387 CCTTGG
TfiI 585 GAATC G^A[AT]TC
805 GAATC
Van91I 318 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
426 CCAGGCGGTGG
XhoII 530 GGATCT [GA]^GATC[CT]
560 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AlwI BanII BbvI
BcnI BglI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI BstXI Cac8I CfoI Cfr13I
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
TseI Tsp45I TspRI