Restriction map for
pF1KE2380
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspI AviII AvrII BamHI BbeI
BciVI BfrI BglI BglII BlnI
BsaBI BseAI BsgI BsiEI BsiWI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstPI Bsu36I ClaI
CpoI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HindIII
HpaI KasI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 1292 GGTACC G^GTACC
AccII 77 CGCG CG^CG
AcsI 1117 AAATTC [GA]^AATT[CT]
AhdI 1228 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1117 AAATTC [GA]^AATT[CT]
Asp718I 1292 GGTACC G^GTACC
AspEI 1228 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1328 TGGCCA TGG^CCA
BbrPI 985 CACGTG CAC^GTG
BbsI 1049 GTCTTC GAAGAC(2/6)
BbvCI 1096 GCTGAGG CCTCAGC(-5/-2)
BcgI 255 CGAGTTTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 506 TGATCA T^GATCA
BfaI 1054 CTAG C^TAG
BlpI 284 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 284 GCTCAGC GC^T[ACGT]AGC
BpuAI 1049 GTCTTC GAAGAC(2/6)
BsaAI 985 CACGTG [CT]AC^GT[GA]
BsaI 423 GAGACC GGTCTC(1/5)
BsaWI 1760 TCCGGT [AT]^CCGG[AT]
BsmI 180 GAATGC GAATGC(1/-1)
BsrFI 337 ACCGGC [GA]^CCGG[CT]
Bst1107I 1318 GTATAC GTA^TAC
BstUI 77 CGCG CG^CG
BstYI 1350 AGATCC [GA]^GATC[CT]
BstZ17I 1318 GTATAC GTA^TAC
CelII 284 GCTCAGC GC^T[ACGT]AGC
Cfr10I 337 ACCGGC [GA]^CCGG[CT]
DraI 1114 TTTAAA TTT^AAA
DraIII 134 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 75 GACGCGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1228 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 829 GAAGAG CTCTTC(1/4)
FbaI 506 TGATCA T^GATCA
HincII 565 GTCAAC GT[CT]^[GA]AC
HindII 565 GTCAAC GT[CT]^[GA]AC
KpnI 1292 GGTACC GGTAC^C
Ksp632I 829 GAAGAG CTCTTC(1/4)
MaeI 1054 CTAG C^TAG
MflI 1350 AGATCC [GA]^GATC[CT]
MluNI 1328 TGGCCA TGG^CCA
MscI 1328 TGGCCA TGG^CCA
MvnI 77 CGCG CG^CG
PmaCI 985 CACGTG CAC^GTG
PmlI 985 CACGTG CAC^GTG
PpuMI 269 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 319 GAGCTC GAGCT^C
ScaI 1333 AGTACT AGT^ACT
SexAI 1313 ACCTGGT A^CC[AT]GGT
SspI 573 AATATT AAT^ATT
SwaI 1113 ATTTAAAT ATTT^AAAT
TaqI 407 TCGA T^CGA
TthHB8I 407 TCGA T^CGA
XcmI 1004 CCAAGTACGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1350 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AccI 148 GTCTAC GT^[AC][GT]AC
1318 GTATAC
BsmBI 688 CGTCTC CGTCTC(1/5)
73 GAGACG
BspHI 929 TCATGA T^CATGA
1730 TCATGA
BstXI 854 CCATCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1448 CCAAAAACATGG
EcoT14I 560 CCATGG C^C[AT][AT]GG
1091 CCATGG
HaeII 397 AGCGCC [GA]GCGC^[CT]
1572 GGCGCT
MspA1I 1393 CAGCTG C[AC]G^C[GT]G
1738 CAGCTG
NcoI 560 CCATGG C^CATGG
1091 CCATGG
PvuII 1393 CAGCTG CAG^CTG
1738 CAGCTG
RcaI 929 TCATGA T^CATGA
1730 TCATGA
SfcI 60 CTGTAG C^T[GA][CT]AG
297 CTACAG
StyI 560 CCATGG C^C[AT][AT]GG
1091 CCATGG
TfiI 737 GATTC G^A[AT]TC
1387 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI Tru9I TseI Tsp45I
Tsp509I TspRI