Restriction map for
pF1KB4089
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# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspI AvaI AviII AvrII
BamHI BanI BbeI BcgI BclI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BsaWI BseAI BsiWI BsmBI
BsoBI BspEI BssHII Bst1107I BstEII
BstPI BstZ17I CelII CpoI DrdI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I FbaI FseI FspI HaeII
HgaI Hin1I KasI KpnI MamI
MluI MroI NaeI NarI NcoI
NgoMIV NotI PacI PaeR7I PflFI
PinAI PmeI PshBI PvuI RsrII
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AclI 3596 AACGTT AA^CGTT
AflIII 453 ACATGT A^C[GA][CT]GT
Alw44I 2789 GTGCAC G^TGCAC
AlwNI 1679 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2557 CCTCAGG CC^T[ACGT]AGG
ApaLI 2789 GTGCAC G^TGCAC
BanII 2362 GAGCTC G[GA]GC[CT]^C
BbrPI 2624 CACGTG CAC^GTG
BbvCI 2839 CCTCAGC CCTCAGC(-5/-2)
BciVI 2563 GTATCC GTATCC(6/5)
BcnI 1944 CCGGG CC^[GC]GG
BsiEI 2421 CGACCG CG[GA][CT]^CG
BsmI 3441 GAATGC GAATGC(1/-1)
Bsp1407I 2541 TGTACA T^GTACA
BspDI 341 ATCGAT AT^CGAT
BspHI 4296 TCATGA T^CATGA
BspLU11I 453 ACATGT A^CATGT
BspMI 50 ACCTGC ACCTGC(4/8)
BsrBI 3274 GAGCGG CCGCTC(-3/-3)
BsrFI 4644 ACCGGC [GA]^CCGG[CT]
BsrGI 2541 TGTACA T^GTACA
BssSI 659 CTCGTG CACGAG(-5/-1)
BstAPI 1555 GCACTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4506 TTCGAA TT^CGAA
Bsu36I 2557 CCTCAGG CC^T[ACGT]AGG
Cfr10I 4644 ACCGGC [GA]^CCGG[CT]
ClaI 341 ATCGAT AT^CGAT
DraI 2257 TTTAAA TTT^AAA
DraII 4385 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 1406 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 44 CCGCGG C^C[GA][CT]GG
Eco81I 2557 CCTCAGG CC^T[ACGT]AGG
EcoO109I 4385 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1997 GAATTC G^AATTC
FauI 43 CCCGC CCCGC(4/6)
HpaI 457 GTTAAC GTT^AAC
KspI 44 CCGCGG CCGC^GG
MfeI 551 CAATTG C^AATTG
MunI 551 CAATTG C^AATTG
NciI 1944 CCGGG CC^[GC]GG
NheI 2796 GCTAGC G^CTAGC
NruI 2418 TCGCGA TCG^CGA
NspV 4506 TTCGAA TT^CGAA
PflMI 700 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2624 CACGTG CAC^GTG
PmlI 2624 CACGTG CAC^GTG
PpuMI 4385 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 2614 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3596 AACGTT AA^CGTT
RcaI 4296 TCATGA T^CATGA
SacI 2362 GAGCTC GAGCT^C
SacII 44 CCGCGG CCGC^GG
SalI 3584 GTCGAC G^TCGAC
SapI 3836 GCTCTTC GCTCTTC(1/4)
ScaI 3181 AGTACT AGT^ACT
SfuI 4506 TTCGAA TT^CGAA
SspBI 2541 TGTACA T^GTACA
SspI 4174 AATATT AAT^ATT
Van91I 700 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1851 CCATGCTCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 173 GTAGAC GT^[AC][GT]AC
3584 GTCGAC
AflII 1219 CTTAAG C^TTAAG
2023 CTTAAG
AhdI 4257 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4601 GACTCCACGTC
Asp700I 2873 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4268 GAAGGAGTTC
AspEI 4257 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4601 GACTCCACGTC
BalI 1588 TGGCCA TGG^CCA
3855 TGGCCA
BfrI 1219 CTTAAG C^TTAAG
2023 CTTAAG
BsaAI 1142 TACGTG [CT]AC^GT[GA]
2624 CACGTG
BsaI 831 GAGACC GGTCTC(1/5)
3624 GAGACC
BsiYI 700 CCACAAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3781 CCCAAACGAGG
BslI 700 CCACAAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3781 CCCAAACGAGG
BsmFI 4112 GTCCC GGGAC(10/14)
4387 GTCCC
CfoI 297 GCGC GCG^C
3445 GCGC
Eam1105I 4257 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4601 GACTCCACGTC
EcoRV 319 GATATC GAT^ATC
1715 GATATC
EcoT22I 1307 ATGCAT ATGCA^T
2885 ATGCAT
HhaI 297 GCGC GCG^C
3445 GCGC
HinP1I 297 GCGC G^CGC
3445 GCGC
HincII 457 GTTAAC GT[CT]^[GA]AC
3584 GTCGAC
HindII 457 GTTAAC GT[CT]^[GA]AC
3584 GTCGAC
HindIII 673 AAGCTT A^AGCTT
4594 AAGCTT
MluNI 1588 TGGCCA TGG^CCA
3855 TGGCCA
MscI 1588 TGGCCA TGG^CCA
3855 TGGCCA
NdeI 329 CATATG CA^TATG
2888 CATATG
NlaIV 2986 GGAGCC GG[ACGT]^[ACGT]CC
4386 GGTCCC
NsiI 1307 ATGCAT ATGCA^T
2885 ATGCAT
PstI 1562 CTGCAG CTGCA^G
1608 CTGCAG
XmnI 2873 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4268 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BbsI
BbvI BfaI BglII BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsmAI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NspI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII