Restriction map for
pF1KE2556
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# NO CUT ENZYMES ( 66 enzymes) #
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Acc65I AccIII AgeI AocI Aor51HI
AscI AseI AsnI Asp718I AspI
AvrII BcgI BglII BlnI BsaWI
BseAI BsmI BspEI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CpoI DraI DrdI Eco47III
Eco81I EcoO65I FseI HincII HindII
HpaI KpnI MfeI MluI MroI
MunI NaeI NcoI NgoMIV NruI
NspV PacI PflFI PinAI PmeI
PshAI PshBI PvuI RsrII SacI
SalI SbfI ScaI SfuI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XbaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 1273 GACGTC GACGT^C
AclI 1812 AACGTT AA^CGTT
AflII 2710 CTTAAG C^TTAAG
AflIII 782 ACATGT A^C[GA][CT]GT
AhdI 1651 GACTACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2701 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1651 GACTACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1632 TGCGCA TGC^GCA
BbrPI 2189 CACGTG CAC^GTG
BciVI 1105 GGATAC GTATCC(6/5)
BclI 465 TGATCA T^GATCA
BfrI 2710 CTTAAG C^TTAAG
BsiWI 1439 CGTACG C^GTACG
BspDI 2774 ATCGAT AT^CGAT
BspLU11I 782 ACATGT A^CATGT
ClaI 2774 ATCGAT AT^CGAT
DraIII 1801 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1517 CGGCCG C^GGCCG
Eam1105I 1651 GACTACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1517 CGGCCG C^GGCCG
Eco52I 1517 CGGCCG C^GGCCG
EcoRI 2705 GAATTC G^AATTC
EcoRV 379 GATATC GAT^ATC
EcoT22I 272 ATGCAT ATGCA^T
FbaI 465 TGATCA T^GATCA
FspI 1632 TGCGCA TGC^GCA
HindIII 1128 AAGCTT A^AGCTT
KspI 1457 CCGCGG CCGC^GG
NdeI 1061 CATATG CA^TATG
NheI 426 GCTAGC G^CTAGC
NotI 1516 GCGGCCGC GC^GGCCGC
NsiI 272 ATGCAT ATGCA^T
PmaCI 2189 CACGTG CAC^GTG
PmlI 2189 CACGTG CAC^GTG
PpuMI 2222 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 1812 AACGTT AA^CGTT
SacII 1457 CCGCGG CCGC^GG
SapI 3015 GAAGAGC GCTCTTC(1/4)
SexAI 346 ACCAGGT A^CC[AT]GGT
SfiI 1936 GGCCTGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 223 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 2174 CCCGGG CCC^GGG
SphI 1389 GCATGC GCATG^C
XmaCI 2174 CCCGGG C^CCGGG
XmaI 2174 CCCGGG C^CCGGG
XmnI 2701 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 35 enzymes) #
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AccI 1180 GTCTAC GT^[AC][GT]AC
1468 GTCTAC
Alw44I 1187 GTGCAC G^TGCAC
1795 GTGCAC
ApaI 2171 GGGCCC GGGCC^C
2257 GGGCCC
ApaLI 1187 GTGCAC G^TGCAC
1795 GTGCAC
BalI 132 TGGCCA TGG^CCA
3031 TGGCCA
BamHI 77 GGATCC G^GATCC
1163 GGATCC
BbeI 1992 GGCGCC GGCGC^C
2178 GGCGCC
BbvCI 1216 CCTCAGC CCTCAGC(-5/-2)
3426 GCTGAGG
BglI 1710 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1937 GCCTGGAGGGC
BlpI 434 GCTTAGC GC^T[ACGT]AGC
1926 GCTCAGC
Bpu1102I 434 GCTTAGC GC^T[ACGT]AGC
1926 GCTCAGC
BsaAI 1009 TACGTG [CT]AC^GT[GA]
2189 CACGTG
BsaBI 3227 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3366 GATCATGATC
BsaI 832 GAGACC GGTCTC(1/5)
2871 GGTCTC
Bsp1407I 617 TGTACA T^GTACA
3479 TGTACA
BspHI 1277 TCATGA T^CATGA
3368 TCATGA
BspMI 929 ACCTGC ACCTGC(4/8)
1680 GCAGGT
BsrBI 1585 GAGCGG CCGCTC(-3/-3)
2166 GAGCGG
BsrDI 774 CATTGC GCAATG(2/0)
2980 GCAATG
BsrGI 617 TGTACA T^GTACA
3479 TGTACA
BssHII 1879 GCGCGC G^CGCGC
2194 GCGCGC
BstAPI 56 GCAAGAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1842 GCACTCGCTGC
CelII 434 GCTTAGC GC^T[ACGT]AGC
1926 GCTCAGC
EcoT14I 681 CCAAGG C^C[AT][AT]GG
2279 CCAAGG
KasI 1992 GGCGCC G^GCGCC
2178 GGCGCC
MamI 3227 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3366 GATCATGATC
MluNI 132 TGGCCA TGG^CCA
3031 TGGCCA
MscI 132 TGGCCA TGG^CCA
3031 TGGCCA
NarI 1992 GGCGCC GG^CGCC
2178 GGCGCC
RcaI 1277 TCATGA T^CATGA
3368 TCATGA
SfoI 1992 GGCGCC GGC^GCC
2178 GGCGCC
SspBI 617 TGTACA T^GTACA
3479 TGTACA
StuI 1282 AGGCCT AGG^CCT
3126 AGGCCT
StyI 681 CCAAGG C^C[AT][AT]GG
2279 CCAAGG
XcmI 2220 CCAGGACCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3318 CCACTGCAGTGCTGG
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# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PaeR7I PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoI
XhoII