Restriction map for
pF1KE2762
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BanI
BbeI BbsI BbvCI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaHI BsaWI
BseAI BseRI BsiWI BsmBI BsmFI
BsmI BspDI BspEI BspHI BsrDI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DdeI
DraI DrdI Eam1105I EarI Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MflI MroI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI Tru9I Tsp509I
TspRI Tth111I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AlwI 752 GGATC GGATC(4/5)
AlwNI 160 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 919 GGGCCC GGGCC^C
AvaII 387 GGACC G^G[AT]CC
BcgI 20 GCAGCGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 442 CTGGAG CTGGAG(16/14)
BsaI 721 GAGACC GGTCTC(1/5)
BsmAI 721 GAGAC GTCTC(1/5)
Bsp1407I 944 TGTACA T^GTACA
BspLU11I 701 ACATGT A^CATGT
BsrBI 520 CCGCTC CCGCTC(-3/-3)
BsrGI 944 TGTACA T^GTACA
BssSI 718 CACGAG CACGAG(-5/-1)
BstXI 434 CCACGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 682 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 923 CCTATGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 536 CCAAGG C^C[AT][AT]GG
HaeII 197 GGCGCT [GA]GCGC^[CT]
HinfI 910 GACTC G^A[ACGT]TC
MaeIII 544 GTGAC ^GT[ACGT]AC
MluI 436 ACGCGT A^CGCGT
NspI 701 ACATGT [GA]CATG^[CT]
PflMI 681 CCACTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 910 GACTC GAGTC(4/5)
PstI 493 CTGCAG CTGCA^G
PvuII 577 CAGCTG CAG^CTG
ScaI 845 AGTACT AGT^ACT
SfcI 493 CTGCAG C^T[GA][CT]AG
SmaI 804 CCCGGG CCC^GGG
SspBI 944 TGTACA T^GTACA
StyI 536 CCAAGG C^C[AT][AT]GG
TaqI 29 TCGA T^CGA
Tsp45I 544 GTGAC ^GT[GC]AC
TthHB8I 29 TCGA T^CGA
Van91I 681 CCACTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 804 CCCGGG C^CCGGG
XmaI 804 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 37 GTCTAC GT^[AC][GT]AC
628 GTCTAC
AflIII 436 ACGCGT A^C[GA][CT]GT
701 ACATGT
BanII 613 GGGCTC G[GA]GC[CT]^C
919 GGGCCC
BbrPI 382 CACGTG CAC^GTG
775 CACGTG
BglI 94 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
586 GCCGACCTGGC
BsaAI 382 CACGTG [CT]AC^GT[GA]
775 CACGTG
BsiEI 517 CGGCCG CG[GA][CT]^CG
584 CGGCCG
BsrI 78 ACTGG ACTGG(1/-1)
749 ACTGG
BstF5I 261 CATCC GGATG(2/0)
801 CATCC
DpnII 753 GATC ^GATC
834 GATC
DsaI 344 CCGTGG C^C[GA][CT]GG
623 CCGTGG
EagI 517 CGGCCG C^GGCCG
584 CGGCCG
EclXI 517 CGGCCG C^GGCCG
584 CGGCCG
Eco52I 517 CGGCCG C^GGCCG
584 CGGCCG
FokI 261 CATCC GGATG(9/13)
801 CATCC
HapII 91 CCGG C^CGG
805 CCGG
HpaII 91 CCGG C^CGG
805 CCGG
MboI 753 GATC ^GATC
834 GATC
MslI 552 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
567 CACGCTGGTG
MspI 91 CCGG C^CGG
805 CCGG
NdeII 753 GATC ^GATC
834 GATC
NlaIII 552 CATG CATG^
702 CATG
NlaIV 918 GGGGCC GG[ACGT]^[ACGT]CC
919 GGGCCC
PmaCI 382 CACGTG CAC^GTG
775 CACGTG
PmlI 382 CACGTG CAC^GTG
775 CACGTG
Sau3AI 753 GATC ^GATC
834 GATC
SfiI 93 GGCCATCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
585 GGCCGACCTGGCC
XcmI 335 CCAACCTGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
641 CCAGCTCCTTCCTGG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaI BalI BbvI BcnI BmyI
BsaJI BsgI BsiHKAI BsiYI BslI
BsoBI Bsp1286I BspMI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
HaeIII HgaI HhaI HinP1I HphI
ItaI MaeII MboII MluNI MnlI
MscI MspA1I MvaI MvnI MwoI
NciI RsaI Sau96I ScrFI SfaNI
SmlI TseI