Restriction map for
pF1KE2078
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Aor51HI
ApaI AscI Asp700I Asp718I AspEI
AspI AviII AvrII BanI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaHI BsiEI BsiWI BspDI
BspLU11I BsrBI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoT22I FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 787 TCCGGA T^CCGGA
AflIII 1741 ACGTGT A^C[GA][CT]GT
Alw44I 1194 GTGCAC G^TGCAC
AocI 505 CCTCAGG CC^T[ACGT]AGG
ApaLI 1194 GTGCAC G^TGCAC
AseI 1687 ATTAAT AT^TAAT
AsnI 1687 ATTAAT AT^TAAT
AvaI 2210 CCCGAG C^[CT]CG[GA]G
BalI 1365 TGGCCA TGG^CCA
BanII 1993 GAGCTC G[GA]GC[CT]^C
BpmI 1019 CTGGAG CTGGAG(16/14)
BsaAI 1740 TACGTG [CT]AC^GT[GA]
BsaBI 1456 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 787 TCCGGA [AT]^CCGG[AT]
BseAI 787 TCCGGA T^CCGGA
BsmBI 2238 GAGACG CGTCTC(1/5)
BsoBI 2210 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1531 TGTACA T^GTACA
BspEI 787 TCCGGA T^CCGGA
BspMI 1702 GCAGGT ACCTGC(4/8)
BsrGI 1531 TGTACA T^GTACA
BstAPI 1537 GCACAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 505 CCTCAGG CC^T[ACGT]AGG
DraIII 1538 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1365 TGGCCA [CT]^GGCC[GA]
Eco81I 505 CCTCAGG CC^T[ACGT]AGG
EcoRI 909 GAATTC G^AATTC
EcoRV 735 GATATC GAT^ATC
FauI 43 GCGGG CCCGC(4/6)
HapII 788 CCGG C^CGG
HpaII 788 CCGG C^CGG
MamI 1456 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1365 TGGCCA TGG^CCA
MroI 787 TCCGGA T^CCGGA
MscI 1365 TGGCCA TGG^CCA
MspI 788 CCGG C^CGG
NspI 600 ACATGC [GA]CATG^[CT]
PflMI 877 CCAAATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 387 GACTC GAGTC(4/5)
PshBI 1687 ATTAAT AT^TAAT
PvuII 647 CAGCTG CAG^CTG
SacI 1993 GAGCTC GAGCT^C
ScaI 279 AGTACT AGT^ACT
SspBI 1531 TGTACA T^GTACA
Tsp45I 1427 GTGAC ^GT[GC]AC
Van91I 877 CCAAATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 183 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AlwNI 1631 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
1829 CAGGGTCTG
BamHI 1168 GGATCC G^GATCC
1885 GGATCC
BbvI 604 GCTGC GCAGC(8/12)
1448 GCTGC
BclI 684 TGATCA T^GATCA
2063 TGATCA
BsaI 2169 GAGACC GGTCTC(1/5)
1106 GGTCTC
BsmI 1444 GAATGC GAATGC(1/-1)
1546 GCATTC
BspHI 468 TCATGA T^CATGA
924 TCATGA
BsrDI 2007 CATTGC GCAATG(2/0)
1695 GCAATG
BssSI 713 CACGAG CACGAG(-5/-1)
389 CTCGTG
BstYI 1168 GGATCC [GA]^GATC[CT]
1885 GGATCC
Cac8I 141 GCGTGC GC[ACGT]^[ACGT]GC
414 GCCAGC
DraII 851 AGGACCC [GA]G^G[ACGT]CC[CT]
1521 AGGTCCT
DsaI 198 CCATGG C^C[GA][CT]GG
345 CCATGG
EarI 225 GAAGAG CTCTTC(1/4)
1338 GAAGAG
EcoO109I 851 AGGACCC [GA]G^G[ACGT]CC[CT]
1521 AGGTCCT
EcoT14I 198 CCATGG C^C[AT][AT]GG
345 CCATGG
FbaI 684 TGATCA T^GATCA
2063 TGATCA
HgaI 2240 GACGC GACGC(5/10)
2199 GCGTC
Ksp632I 225 GAAGAG CTCTTC(1/4)
1338 GAAGAG
MflI 1168 GGATCC [GA]^GATC[CT]
1885 GGATCC
MspA1I 647 CAGCTG C[AC]G^C[GT]G
2203 CAGCGG
NcoI 198 CCATGG C^CATGG
345 CCATGG
PpuMI 851 AGGACCC [GA]G^G[AT]CC[CT]
1521 AGGTCCT
RcaI 468 TCATGA T^CATGA
924 TCATGA
SfcI 1302 CTGTAG C^T[GA][CT]AG
1422 CTATAG
SmlI 590 CTCAAG C^T[CT][GA]AG
1330 CTTGAG
SspI 701 AATATT AAT^ATT
880 AATATT
StyI 198 CCATGG C^C[AT][AT]GG
345 CCATGG
TaqI 1454 TCGA T^CGA
1863 TCGA
TseI 604 GCTGC G^C[AT]GC
1448 GCTGC
TthHB8I 1454 TCGA T^CGA
1863 TCGA
XcmI 384 CCAGACTCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1368 CCAACCTATTTGTGG
XhoII 1168 GGATCC [GA]^GATC[CT]
1885 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbsI BfaI
BmyI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI Cfr13I DdeI DpnII DraI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I Tsp509I TspRI