##################################
# NO CUT ENZYMES (  205 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DdeI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoT14I     EcoT22I     FauI        FbaI        Fnu4HI      
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaI        HpaII       HphI        ItaI        
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacII       SalI        SbfI        ScaI        SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AciI          205  GCGG                 CCGC(-3/-1)
AfaI          314  GTAC                 GT^AC
AspHI         125  GAGCTC               G[AT]GC[AT]^C
BfaI          211  CTAG                 C^TAG
BpmI          156  CTCCAG               CTGGAG(16/14)
BsaJI         150  CCCAGG               C^C[ACGT][ACGT]GG
BsiHKAI       125  GAGCTC               G[AT]GC[AT]^C
BsiYI          51  CCTGAATGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           51  CCTGAATGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         304  GAGAC                GTCTC(1/5)
BsmBI         304  GAGACG               CGTCTC(1/5)
BsmFI          29  GTCCC                GGGAC(10/14)
BspHI         333  TCATGA               T^CATGA
BstF5I        162  CATCC                GGATG(2/0)
DpnII          10  GATC                 ^GATC
EcoRV         289  GATATC               GAT^ATC
FokI          162  CATCC                GGATG(9/13)
HaeIII        256  GGCC                 GG^CC
HindIII       188  AAGCTT               A^AGCTT
MaeI          211  CTAG                 C^TAG
MboI           10  GATC                 ^GATC
MnlI          139  GAGG                 CCTC(7/6)
MslI          328  CACAGTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII          10  GATC                 ^GATC
NlaIII        334  CATG                 CATG^
RcaI          333  TCATGA               T^CATGA
RsaI          314  GTAC                 GT^AC
SacI          125  GAGCTC               GAGCT^C
SapI           16  GAAGAGC              GCTCTTC(1/4)
Sau3AI         10  GATC                 ^GATC
SexAI         296  ACCTGGT              A^CC[AT]GGT
SfaNI         161  GCATC                GCATC(5/9)
Tsp45I         35  GTCAC                ^GT[GC]AC
TspRI         318  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AluI          126  AGCT                 AG^CT
              189  AGCT                
AvaII          28  GGTCC                G^G[AT]CC
              207  GGTCC               
BanII          19  GAGCCC               G[GA]GC[CT]^C
              125  GAGCTC              
BssKI         151  CCAGG                ^CC[ACGT]GG
              297  CCTGG               
BstNI         151  CCAGG                CC^[AT]GG
              297  CCTGG               
Cfr13I         28  GGTCC                G^G[ACGT]CC
              207  GGTCC               
EarI           16  GAAGAG               CTCTTC(1/4)
              136  GAAGAG              
EcoRII        151  CCAGG                ^CC[AT]GG
              297  CCTGG               
Ksp632I        16  GAAGAG               CTCTTC(1/4)
              136  GAAGAG              
MboII          16  GAAGA                GAAGA(8/7)
              136  GAAGA               
MvaI          151  CCAGG                CC^[AT]GG
              297  CCTGG               
MwoI          190  GCTTTTGTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              248  GCAGGAAAGGC         
Sau96I         28  GGTCC                G^G[ACGT]CC
              207  GGTCC               
ScrFI         151  CCAGG                CC^[ACGT]GG
              297  CCTGG               


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

BmyI        Bsp1286I    BsrI        MaeIII      NlaIV