Restriction map for
pF1KE3379
##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AccI AclI AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I AviII AvrII BbeI BcgI
BciVI BclI BfrI BglII BlnI
BsaBI BsiWI BsmI BspDI BspHI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI DraI Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FspI
HincII HindII HpaI KasI MamI
MfeI MluI MunI NarI NdeI
NheI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI SalI
ScaI SexAI SfoI SfuI SnaBI
SpeI SspI SwaI Van91I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 2372 GGTACC G^GTACC
AccIII 3204 TCCGGA T^CCGGA
AcsI 2459 GAATTT [GA]^AATT[CT]
AocI 667 CCTGAGG CC^T[ACGT]AGG
ApoI 2459 GAATTT [GA]^AATT[CT]
Asp718I 2372 GGTACC G^GTACC
AspI 1336 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2933 GGATCC G^GATCC
BbvCI 1192 GCTGAGG CCTCAGC(-5/-2)
BsaWI 3204 TCCGGA [AT]^CCGG[AT]
BseAI 3204 TCCGGA T^CCGGA
BsmBI 3307 GAGACG CGTCTC(1/5)
Bsp1407I 3152 TGTACA T^GTACA
BspEI 3204 TCCGGA T^CCGGA
BspLU11I 2535 ACATGT A^CATGT
BsrGI 3152 TGTACA T^GTACA
BstXI 71 CCATGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 667 CCTGAGG CC^T[ACGT]AGG
CpoI 1545 CGGACCG CG^G[AT]CCG
DraIII 59 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 667 CCTGAGG CC^T[ACGT]AGG
FseI 1587 GGCCGGCC GGCCGG^CC
HaeII 2679 GGCGCT [GA]GCGC^[CT]
KpnI 2372 GGTACC GGTAC^C
KspI 2095 CCGCGG CCGC^GG
MroI 3204 TCCGGA T^CCGGA
MseI 113 TTAA T^TAA
NotI 1224 GCGGCCGC GC^GGCCGC
PflFI 1336 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 1545 CGGACCG CG^G[AT]CCG
SacII 2095 CCGCGG CCGC^GG
SapI 1404 GCTCTTC GCTCTTC(1/4)
SbfI 1392 CCTGCAGG CCTGCA^GG
SmlI 2664 CTTGAG C^T[CT][GA]AG
SphI 896 GCATGC GCATG^C
Sse8387I 1392 CCTGCAGG CCTGCA^GG
SspBI 3152 TGTACA T^GTACA
Tru9I 113 TTAA T^TAA
Tsp509I 2460 AATT ^AATT
Tth111I 1336 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 68 CCACCATGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 945 GACGTC GACGT^C
3232 GACGTC
AflIII 1040 ACACGT A^C[GA][CT]GT
2535 ACATGT
BbrPI 1539 CACGTG CAC^GTG
2470 CACGTG
BlpI 481 GCTGAGC GC^T[ACGT]AGC
3144 GCTAAGC
Bpu1102I 481 GCTGAGC GC^T[ACGT]AGC
3144 GCTAAGC
BsrBI 1026 CCGCTC CCGCTC(-3/-3)
1135 GAGCGG
BsrDI 1360 CATTGC GCAATG(2/0)
2139 GCAATG
BstAPI 1395 GCAGGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2355 GCAGAAGGTGC
CelII 481 GCTGAGC GC^T[ACGT]AGC
3144 GCTAAGC
DrdI 304 GACCAGTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3001 GACGAGGGTGTC
NaeI 1319 GCCGGC GCC^GGC
1588 GCCGGC
NgoMIV 1319 GCCGGC G^CCGGC
1588 GCCGGC
NspI 896 GCATGC [GA]CATG^[CT]
2535 ACATGT
PmaCI 1539 CACGTG CAC^GTG
2470 CACGTG
PmlI 1539 CACGTG CAC^GTG
2470 CACGTG
SfiI 1927 GGCCTGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1980 GGCCAAGCTGGCC
SgrAI 1219 CACCGGCG C[GA]^CCGG[CT]G
1553 CACCGGCG
SmaI 1646 CCCGGG CCC^GGG
1960 CCCGGG
StuI 1185 AGGCCT AGG^CCT
2549 AGGCCT
XmaCI 1646 CCCGGG C^CCGGG
1960 CCCGGG
XmaI 1646 CCCGGG C^CCGGG
1960 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI ApaI AspEI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI Eam1105I EarI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XhoII