Restriction map for
pF1KB4192
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# NO CUT ENZYMES ( 110 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BbrPI BbvCI BcgI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspEI BspLU11I
BsrGI BssHII Bst1107I BstBI BstYI
BstZ17I CelII CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI MfeI MflI MluI
MroI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI PvuII RsrII SalI SbfI
ScaI SexAI SfuI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 685 GACGTC GACGT^C
AfaI 510 GTAC GT^AC
AflIII 980 ACGTGT A^C[GA][CT]GT
AlwI 639 GATCC GGATC(4/5)
AocI 133 CCTGAGG CC^T[ACGT]AGG
BalI 351 TGGCCA TGG^CCA
BbsI 853 GTCTTC GAAGAC(2/6)
BfaI 215 CTAG C^TAG
BpuAI 853 GTCTTC GAAGAC(2/6)
BsaI 822 GGTCTC GGTCTC(1/5)
BsmI 1353 GAATGC GAATGC(1/-1)
BspDI 232 ATCGAT AT^CGAT
BspHI 956 TCATGA T^CATGA
BspMI 914 GCAGGT ACCTGC(4/8)
BsrDI 867 CATTGC GCAATG(2/0)
BstAPI 434 GCAGCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 648 CCACCACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 133 CCTGAGG CC^T[ACGT]AGG
ClaI 232 ATCGAT AT^CGAT
Eco81I 133 CCTGAGG CC^T[ACGT]AGG
EcoT14I 1105 CCAAGG C^C[AT][AT]GG
KspI 1018 CCGCGG CCGC^GG
MaeI 215 CTAG C^TAG
MluNI 351 TGGCCA TGG^CCA
MscI 351 TGGCCA TGG^CCA
MseI 734 TTAA T^TAA
PflMI 1230 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 681 GACTGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 956 TCATGA T^CATGA
RsaI 510 GTAC GT^AC
SacI 1174 GAGCTC GAGCT^C
SacII 1018 CCGCGG CCGC^GG
SfaNI 1273 GATGC GCATC(5/9)
SfcI 258 CTACAG C^T[GA][CT]AG
SfiI 1083 GGCCCCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1317 CACCGGCG C[GA]^CCGG[CT]G
SmlI 157 CTCAAG C^T[CT][GA]AG
SspI 343 AATATT AAT^ATT
StuI 590 AGGCCT AGG^CCT
StyI 1105 CCAAGG C^C[AT][AT]GG
Tru9I 734 TTAA T^TAA
Tsp509I 961 AATT ^AATT
Van91I 1230 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 388 GTCTAC GT^[AC][GT]AC
544 GTAGAC
AlwNI 435 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1231 CAGAGCCTG
AviII 442 TGCGCA TGC^GCA
1343 TGCGCA
BbeI 658 GGCGCC GGCGC^C
1240 GGCGCC
BciVI 138 GGATAC GTATCC(6/5)
1469 GTATCC
BpmI 1407 CTCCAG CTGGAG(16/14)
1039 CTGGAG
BsaBI 225 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
573 GATCGACATC
BstEII 162 GGTCACC G^GT[ACGT]ACC
453 GGTCACC
BstF5I 705 CATCC GGATG(2/0)
1272 GGATG
BstPI 162 GGTCACC G^GT[ACGT]ACC
453 GGTCACC
EcoO65I 162 GGTCACC G^GT[ACGT]ACC
453 GGTCACC
FokI 705 CATCC GGATG(9/13)
1272 GGATG
FspI 442 TGCGCA TGC^GCA
1343 TGCGCA
HaeII 658 GGCGCC [GA]GCGC^[CT]
1240 GGCGCC
KasI 658 GGCGCC G^GCGCC
1240 GGCGCC
MamI 225 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
573 GATCGACATC
NarI 658 GGCGCC GG^CGCC
1240 GGCGCC
PpuMI 20 GGGTCCT [GA]G^G[AT]CC[CT]
1207 GGGACCT
SapI 720 GCTCTTC GCTCTTC(1/4)
1392 GCTCTTC
SfoI 658 GGCGCC GGC^GCC
1240 GGCGCC
SmaI 630 CCCGGG CCC^GGG
1088 CCCGGG
TfiI 174 GAATC G^A[AT]TC
230 GAATC
XmaCI 630 CCCGGG C^CCGGG
1088 CCCGGG
XmaI 630 CCCGGG C^CCGGG
1088 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
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AccII AciI AcyI AluI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BglI BmyI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrFI
BsrI BssKI BssSI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI TaqI TseI Tsp45I TspRI
TthHB8I