Restriction map for
pF1KB6562
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AfaI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbsI BbvCI BcgI BciVI BfaI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI Eam1105I Eco47III Eco81I
EcoO65I EcoT22I FauI FseI FspI
HgaI HindIII HpaI KpnI KspI
MaeI MamI MfeI MluI MroI
MslI MunI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 543 GTCTAC GT^[AC][GT]AC
AcyI 81 GGCGCC G[GA]^CG[CT]C
Alw44I 497 GTGCAC G^TGCAC
ApaLI 497 GTGCAC G^TGCAC
AspHI 497 GTGCAC G[AT]GC[AT]^C
BalI 899 TGGCCA TGG^CCA
BanII 12 GAGCCC G[GA]GC[CT]^C
BbeI 81 GGCGCC GGCGC^C
BclI 942 TGATCA T^GATCA
BglI 14 GCCCGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 263 AGATCT A^GATCT
BpmI 870 CTGGAG CTGGAG(16/14)
BsaHI 81 GGCGCC G[GA]^CG[CT]C
BseRI 329 GAGGAG GAGGAG(10/8)
BsiEI 95 CGGCCG CG[GA][CT]^CG
BsiHKAI 497 GTGCAC G[AT]GC[AT]^C
BsmAI 222 GAGAC GTCTC(1/5)
BsmFI 75 GTCCC GGGAC(10/14)
BsrI 281 ACTGG ACTGG(1/-1)
DraII 73 GGGTCCC [GA]G^G[ACGT]CC[CT]
EagI 95 CGGCCG C^GGCCG
EarI 502 CTCTTC CTCTTC(1/4)
EclXI 95 CGGCCG C^GGCCG
Eco52I 95 CGGCCG C^GGCCG
EcoNI 1041 CCTTACGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 73 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 685 GATATC GAT^ATC
EcoT14I 312 CCTTGG C^C[AT][AT]GG
FbaI 942 TGATCA T^GATCA
HaeII 81 GGCGCC [GA]GCGC^[CT]
Hin1I 81 GGCGCC G[GA]^CG[CT]C
HphI 514 GGTGA GGTGA(8/7)
KasI 81 GGCGCC G^GCGCC
Ksp632I 502 CTCTTC CTCTTC(1/4)
MaeII 1116 ACGT A^CGT
MluNI 899 TGGCCA TGG^CCA
MscI 899 TGGCCA TGG^CCA
NarI 81 GGCGCC GG^CGCC
PpuMI 73 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 293 CTGCAG CTGCA^G
PvuII 979 CAGCTG CAG^CTG
SfoI 81 GGCGCC GGC^GCC
StyI 312 CCTTGG C^C[AT][AT]GG
TaqI 1193 TCGA T^CGA
TthHB8I 1193 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvaI 15 CCCGAG C^[CT]CG[GA]G
1011 CCCGAG
AvaII 74 GGTCC G^G[AT]CC
357 GGTCC
BmyI 12 GAGCCC G[AGT]GC[ACT]^C
497 GTGCAC
BsoBI 15 CCCGAG C^[CT]CG[GA]G
1011 CCCGAG
Bsp1286I 12 GAGCCC G[AGT]GC[ACT]^C
497 GTGCAC
BsrBI 858 CCGCTC CCGCTC(-3/-3)
18 GAGCGG
BstNI 85 CCAGG CC^[AT]GG
1152 CCTGG
CfoI 82 GCGC GCG^C
833 GCGC
EaeI 95 CGGCCG [CT]^GGCC[GA]
899 TGGCCA
EcoRI 395 GAATTC G^AATTC
1003 GAATTC
EcoRII 85 CCAGG ^CC[AT]GG
1152 CCTGG
HhaI 82 GCGC GCG^C
833 GCGC
HinP1I 82 GCGC G^CGC
833 GCGC
MaeIII 515 GTGAC ^GT[ACGT]AC
737 GTGAC
MvaI 85 CCAGG CC^[AT]GG
1152 CCTGG
NspI 107 GCATGC [GA]CATG^[CT]
1132 GCATGC
PleI 766 GACTC GAGTC(4/5)
244 GAGTC
SphI 107 GCATGC GCATG^C
1132 GCATGC
SspI 700 AATATT AAT^ATT
724 AATATT
TfiI 183 GAATC G^A[AT]TC
1054 GAATC
Tsp45I 515 GTGAC ^GT[GC]AC
737 GTGAC
XcmI 360 CCACAAACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
698 CCAATATTCTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BanI BbvI BcnI BsaJI BsiYI
BslI BssKI BstF5I BstYI Cac8I
Cfr13I DdeI DpnII Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII ItaI MboI MboII MflI
MnlI MseI MspA1I MspI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI SfcI Tru9I
TseI Tsp509I TspRI XhoII