Restriction map for
pF1KE0282
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaHI BseAI BseRI BsiWI BsmBI
BsmI Bsp1407I BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I CpoI
DraI DraII DraIII DrdI DsaI
Eco47III Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII Hin1I HpaI
KasI KpnI KspI MluI MluNI
MroI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacII SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccII 12 CGCG CG^CG
AfaI 412 GTAC GT^AC
AflIII 151 ACACGT A^C[GA][CT]GT
AhdI 1282 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1282 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 216 GAGCTC G[AT]GC[AT]^C
BfaI 52 CTAG C^TAG
BglI 359 GCCCAACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1030 AGATCT A^GATCT
BsaBI 187 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 929 GGTCTC GGTCTC(1/5)
BsaWI 1177 TCCGGT [AT]^CCGG[AT]
BsgI 548 CTGCAC GTGCAG(16/14)
BsiEI 8 CGGCCG CG[GA][CT]^CG
BsiHKAI 216 GAGCTC G[AT]GC[AT]^C
BsmAI 930 GTCTC GTCTC(1/5)
BspDI 1010 ATCGAT AT^CGAT
BspMI 926 GCAGGT ACCTGC(4/8)
BstNI 465 CCAGG CC^[AT]GG
BstUI 12 CGCG CG^CG
Cac8I 355 GCTGGC GC[ACGT]^[ACGT]GC
ClaI 1010 ATCGAT AT^CGAT
EaeI 8 CGGCCG [CT]^GGCC[GA]
EagI 8 CGGCCG C^GGCCG
Eam1105I 1282 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 8 CGGCCG C^GGCCG
Eco52I 8 CGGCCG C^GGCCG
EcoRII 465 CCAGG ^CC[AT]GG
EcoT14I 1127 CCAAGG C^C[AT][AT]GG
HgaI 735 GCGTC GACGC(5/10)
HincII 528 GTCGAC GT[CT]^[GA]AC
HindII 528 GTCGAC GT[CT]^[GA]AC
HindIII 540 AAGCTT A^AGCTT
HinfI 1216 GAATC G^A[ACGT]TC
HphI 1279 GGTGA GGTGA(8/7)
MaeI 52 CTAG C^TAG
MamI 187 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 533 CAATTG C^AATTG
MspA1I 353 CCGCTG C[AC]G^C[GT]G
MunI 533 CAATTG C^AATTG
MvaI 465 CCAGG CC^[AT]GG
MvnI 12 CGCG CG^CG
PflMI 630 CCATTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 412 GTAC GT^AC
SacI 216 GAGCTC GAGCT^C
SalI 528 GTCGAC G^TCGAC
SapI 213 GAAGAGC GCTCTTC(1/4)
SfcI 677 CTGTAG C^T[GA][CT]AG
SmaI 617 CCCGGG CCC^GGG
SmlI 897 CTCAAG C^T[CT][GA]AG
SspI 107 AATATT AAT^ATT
StyI 1127 CCAAGG C^C[AT][AT]GG
TfiI 1216 GAATC G^A[AT]TC
Van91I 630 CCATTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 336 CCAGCAGTCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 617 CCCGGG C^CCGGG
XmaI 617 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 528 GTCGAC GT^[AC][GT]AC
890 GTAGAC
AcsI 179 AAATTC [GA]^AATT[CT]
383 AAATTC
ApoI 179 AAATTC [GA]^AATT[CT]
383 AAATTC
AvaI 617 CCCGGG C^[CT]CG[GA]G
932 CTCGGG
BanII 216 GAGCTC G[GA]GC[CT]^C
848 GGGCTC
BmyI 216 GAGCTC G[AGT]GC[ACT]^C
848 GGGCTC
BsoBI 617 CCCGGG C^[CT]CG[GA]G
932 CTCGGG
Bsp1286I 216 GAGCTC G[AGT]GC[ACT]^C
848 GGGCTC
BspHI 183 TCATGA T^CATGA
227 TCATGA
BsrI 612 CCAGT ACTGG(1/-1)
1256 CCAGT
BssSI 91 CACGAG CACGAG(-5/-1)
851 CTCGTG
BstF5I 24 GGATG GGATG(2/0)
470 GGATG
BstYI 1030 AGATCT [GA]^GATC[CT]
1147 GGATCT
CfoI 13 GCGC GCG^C
69 GCGC
FokI 24 GGATG GGATG(9/13)
470 GGATG
HhaI 13 GCGC GCG^C
69 GCGC
HinP1I 13 GCGC G^CGC
69 GCGC
MflI 1030 AGATCT [GA]^GATC[CT]
1147 GGATCT
MwoI 313 GCTCGTTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
359 GCCCAACAGGC
RcaI 183 TCATGA T^CATGA
227 TCATGA
SfaNI 607 GATGC GCATC(5/9)
920 GATGC
TspRI 1212 CACTG CA[GC]TG[ACGT][ACGT]^
1257 CAGTG
XhoII 1030 AGATCT [GA]^GATC[CT]
1147 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AlwI Asp700I AvaII
BbsI BbvI BcnI BpuAI BsaJI
BsiYI BslI BsmFI BssKI Cfr13I
DdeI DpnII EarI Fnu4HI HaeIII
HapII HpaII ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MseI
MspI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TaqI Tru9I
TseI Tsp45I Tsp509I TthHB8I XmnI