Restriction map for
pF1KE6687
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
AocI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlpI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaWI
BseAI BseRI BsiWI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
DsaI Eam1105I EarI Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MboII MfeI MroI MseI MslI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp509I TspRI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflIII 476 ACGCGT A^C[GA][CT]GT
Alw44I 592 GTGCAC G^TGCAC
AlwI 177 GGATC GGATC(4/5)
AlwNI 316 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 592 GTGCAC G^TGCAC
AvrII 18 CCTAGG C^CTAGG
BalI 236 TGGCCA TGG^CCA
BanI 105 GGCACC G^G[CT][GA]CC
BlnI 18 CCTAGG C^CTAGG
BsaAI 514 TACGTG [CT]AC^GT[GA]
BsaBI 651 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 278 GTGCAG GTGCAG(16/14)
BsmAI 187 GAGAC GTCTC(1/5)
BsmBI 187 GAGACG CGTCTC(1/5)
BspMI 119 ACCTGC ACCTGC(4/8)
BsrI 660 CCAGT ACTGG(1/-1)
BstAPI 566 GCATGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 24 GGTCACC G^GT[ACGT]ACC
BstPI 24 GGTCACC G^GT[ACGT]ACC
BstYI 177 GGATCT [GA]^GATC[CT]
DdeI 409 CTAAG C^T[ACGT]AG
DraII 296 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 296 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 24 GGTCACC G^GT[ACGT]ACC
HgaI 189 GACGC GACGC(5/10)
HinfI 651 GATTC G^A[ACGT]TC
HphI 26 TCACC GGTGA(8/7)
MaeII 515 ACGT A^CGT
MamI 651 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 177 GGATCT [GA]^GATC[CT]
MluI 476 ACGCGT A^CGCGT
MluNI 236 TGGCCA TGG^CCA
MscI 236 TGGCCA TGG^CCA
NaeI 73 GCCGGC GCC^GGC
NgoMIV 73 GCCGGC G^CCGGC
PpuMI 296 AGGACCT [GA]G^G[AT]CC[CT]
TaqI 140 TCGA T^CGA
TfiI 651 GATTC G^A[AT]TC
Tsp45I 25 GTCAC ^GT[GC]AC
TthHB8I 140 TCGA T^CGA
XhoII 177 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 474 GTAC GT^AC
663 GTAC
BcnI 102 CCCGG CC^[GC]GG
262 CCCGG
BfaI 19 CTAG C^TAG
367 CTAG
BsiEI 71 CGGCCG CG[GA][CT]^CG
331 CGGCCG
BssSI 546 CTCGTG CACGAG(-5/-1)
690 CTCGTG
BstF5I 96 CATCC GGATG(2/0)
657 CATCC
DpnII 178 GATC ^GATC
357 GATC
EagI 71 CGGCCG C^GGCCG
331 CGGCCG
EclXI 71 CGGCCG C^GGCCG
331 CGGCCG
Eco52I 71 CGGCCG C^GGCCG
331 CGGCCG
EcoT14I 18 CCTAGG C^C[AT][AT]GG
167 CCAAGG
FokI 96 CATCC GGATG(9/13)
657 CATCC
MaeI 19 CTAG C^TAG
367 CTAG
MaeIII 25 GTCAC ^GT[ACGT]AC
432 GTTAC
MboI 178 GATC ^GATC
357 GATC
NciI 102 CCCGG CC^[GC]GG
262 CCCGG
NdeII 178 GATC ^GATC
357 GATC
RsaI 474 GTAC GT^AC
663 GTAC
Sau3AI 178 GATC ^GATC
357 GATC
StyI 18 CCTAGG C^C[AT][AT]GG
167 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AluI Aor51HI AspHI
AvaI AvaII BbvI BmyI BsaJI
BsiHKAI BsiYI BslI BsoBI Bsp1286I
BsrFI BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I EaeI Eco47III
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII ItaI
MnlI MspA1I MspI MvaI MvnI
MwoI NlaIII NlaIV PvuII Sau96I
ScrFI TseI