Restriction map for
pF1KE3196
##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI AhdI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BbeI BcgI BciVI BclI
BfrI BglII BlnI BsaBI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eam1105I Eco47III Eco81I EcoNI EcoRI
EcoRV FbaI FseI HincII HindII
HindIII HpaI KasI KpnI MamI
MfeI MluI MseI MunI NarI
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tth111I Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccIII 359 TCCGGA T^CCGGA
Alw44I 1318 GTGCAC G^TGCAC
AlwNI 587 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 951 GGGCCC GGGCC^C
ApaLI 1318 GTGCAC G^TGCAC
AviII 866 TGCGCA TGC^GCA
BalI 1169 TGGCCA TGG^CCA
BamHI 261 GGATCC G^GATCC
BbrPI 367 CACGTG CAC^GTG
BbsI 652 GAAGAC GAAGAC(2/6)
BbvCI 385 GCTGAGG CCTCAGC(-5/-2)
BglI 275 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 756 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 756 GCTCAGC GC^T[ACGT]AGC
BpuAI 652 GAAGAC GAAGAC(2/6)
BsaI 550 GAGACC GGTCTC(1/5)
BsaWI 359 TCCGGA [AT]^CCGG[AT]
BseAI 359 TCCGGA T^CCGGA
BsmBI 497 GAGACG CGTCTC(1/5)
BsmI 1201 GAATGC GAATGC(1/-1)
BspEI 359 TCCGGA T^CCGGA
BsrDI 777 CATTGC GCAATG(2/0)
BstEII 642 GGTCACC G^GT[ACGT]ACC
BstPI 642 GGTCACC G^GT[ACGT]ACC
BstXI 621 CCATTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 261 GGATCC [GA]^GATC[CT]
CelII 756 GCTCAGC GC^T[ACGT]AGC
DpnII 262 GATC ^GATC
EagI 879 CGGCCG C^GGCCG
EclXI 879 CGGCCG C^GGCCG
Eco52I 879 CGGCCG C^GGCCG
EcoO65I 642 GGTCACC G^GT[ACGT]ACC
EcoT22I 1111 ATGCAT ATGCA^T
FspI 866 TGCGCA TGC^GCA
MboI 262 GATC ^GATC
MflI 261 GGATCC [GA]^GATC[CT]
MluNI 1169 TGGCCA TGG^CCA
MroI 359 TCCGGA T^CCGGA
MscI 1169 TGGCCA TGG^CCA
MslI 405 CACGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 1189 CATATG CA^TATG
NdeII 262 GATC ^GATC
NsiI 1111 ATGCAT ATGCA^T
PmaCI 367 CACGTG CAC^GTG
PmlI 367 CACGTG CAC^GTG
PpuMI 223 GGGACCC [GA]G^G[AT]CC[CT]
PstI 98 CTGCAG CTGCA^G
Sau3AI 262 GATC ^GATC
SgrAI 87 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 278 CCCGGG CCC^GGG
SmlI 8 CTCAAG C^T[CT][GA]AG
StuI 344 AGGCCT AGG^CCT
TaqI 251 TCGA T^CGA
Tsp509I 1286 AATT ^AATT
TthHB8I 251 TCGA T^CGA
XhoII 261 GGATCC [GA]^GATC[CT]
XmaCI 278 CCCGGG C^CCGGG
XmaI 278 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 262 GATCC GGATC(4/5)
261 GGATC
AvaI 57 CCCGAG C^[CT]CG[GA]G
278 CCCGGG
BanI 841 GGCACC G^G[CT][GA]CC
1011 GGTGCC
BfaI 793 CTAG C^TAG
1033 CTAG
BpmI 494 CTGGAG CTGGAG(16/14)
979 CTGGAG
BsaAI 367 CACGTG [CT]AC^GT[GA]
1123 TACGTG
BseRI 457 GAGGAG GAGGAG(10/8)
982 GAGGAG
BsiEI 451 CGGTCG CG[GA][CT]^CG
879 CGGCCG
BsmFI 223 GGGAC GGGAC(10/14)
473 GGGAC
BsoBI 57 CCCGAG C^[CT]CG[GA]G
278 CCCGGG
BssSI 511 CACGAG CACGAG(-5/-1)
1142 CTCGTG
DraII 223 GGGACCC [GA]G^G[ACGT]CC[CT]
950 AGGGCCC
DraIII 511 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
967 CACCTTGTG
EaeI 879 CGGCCG [CT]^GGCC[GA]
1169 TGGCCA
EcoO109I 223 GGGACCC [GA]G^G[ACGT]CC[CT]
950 AGGGCCC
HaeII 205 AGCGCC [GA]GCGC^[CT]
216 AGCGCC
HinfI 36 GACTC G^A[ACGT]TC
363 GAGTC
MaeI 793 CTAG C^TAG
1033 CTAG
MaeII 368 ACGT A^CGT
1124 ACGT
NaeI 137 GCCGGC GCC^GGC
689 GCCGGC
NcoI 1085 CCATGG C^CATGG
1172 CCATGG
NgoMIV 137 GCCGGC G^CCGGC
689 GCCGGC
PleI 36 GACTC GAGTC(4/5)
363 GAGTC
SapI 1399 GAAGAGC GCTCTTC(1/4)
354 GCTCTTC
SfcI 98 CTGCAG C^T[GA][CT]AG
899 CTGTAG
XcmI 220 CCAGGGACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1324 CCAAACTACTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaII BanII BbvI BcnI BmyI
BsaHI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DsaI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI Ksp632I KspI MaeIII
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PvuII SacII Sau96I ScrFI SfaNI
StyI TseI Tsp45I TspRI