Restriction map for
pF1KE2307
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbsI BbvI
BcgI BcnI BfrI BglI BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I Cac8I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FauI Fnu4HI
FseI FspI HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
ItaI KasI KpnI KspI MaeII
MamI MluI MroI MslI MspA1I
MspI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI TseI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 425 GTAGAC GT^[AC][GT]AC
AcsI 280 GAATTC [GA]^AATT[CT]
AfaI 252 GTAC GT^AC
AflIII 609 ACATGT A^C[GA][CT]GT
AlwI 288 GATCC GGATC(4/5)
AlwNI 581 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 824 AGCGCT AGC^GCT
ApoI 280 GAATTC [GA]^AATT[CT]
AvaII 736 GGTCC G^G[AT]CC
BanI 362 GGTGCC G^G[CT][GA]CC
BanII 683 GGGCTC G[GA]GC[CT]^C
BbvCI 725 CCTCAGC CCTCAGC(-5/-2)
BciVI 807 GTATCC GTATCC(6/5)
BclI 348 TGATCA T^GATCA
BfaI 91 CTAG C^TAG
BglII 442 AGATCT A^GATCT
BmyI 683 GGGCTC G[AGT]GC[ACT]^C
BseRI 495 GAGGAG GAGGAG(10/8)
Bsp1286I 683 GGGCTC G[AGT]GC[ACT]^C
BspLU11I 609 ACATGT A^CATGT
BssSI 41 CTCGTG CACGAG(-5/-1)
BstXI 641 CCAAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 442 AGATCT [GA]^GATC[CT]
CfoI 825 GCGC GCG^C
Cfr13I 736 GGTCC G^G[ACGT]CC
DraII 735 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eco47III 824 AGCGCT AGC^GCT
EcoO109I 735 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 280 GAATTC G^AATTC
FbaI 348 TGATCA T^GATCA
HaeII 824 AGCGCT [GA]GCGC^[CT]
HhaI 825 GCGC GCG^C
HinP1I 825 GCGC G^CGC
HphI 597 TCACC GGTGA(8/7)
MaeI 91 CTAG C^TAG
MflI 442 AGATCT [GA]^GATC[CT]
MseI 164 TTAA T^TAA
NspI 609 ACATGT [GA]CATG^[CT]
PleI 199 GAGTC GAGTC(4/5)
PpuMI 735 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 384 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 252 GTAC GT^AC
SapI 592 GCTCTTC GCTCTTC(1/4)
Sau96I 736 GGTCC G^G[ACGT]CC
SfcI 587 CTGTAG C^T[GA][CT]AG
SmlI 522 CTCAAG C^T[CT][GA]AG
TfiI 583 GATTC G^A[AT]TC
Tru9I 164 TTAA T^TAA
XcmI 366 CCAAAGCCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 442 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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BalI 170 TGGCCA TGG^CCA
650 TGGCCA
BsrI 648 ACTGG ACTGG(1/-1)
292 CCAGT
EaeI 170 TGGCCA [CT]^GGCC[GA]
650 TGGCCA
EarI 393 CTCTTC CTCTTC(1/4)
593 CTCTTC
HaeIII 171 GGCC GG^CC
651 GGCC
HinfI 199 GAGTC G^A[ACGT]TC
583 GATTC
Ksp632I 393 CTCTTC CTCTTC(1/4)
593 CTCTTC
MfeI 548 CAATTG C^AATTG
704 CAATTG
MluNI 170 TGGCCA TGG^CCA
650 TGGCCA
MscI 170 TGGCCA TGG^CCA
650 TGGCCA
MunI 548 CAATTG C^AATTG
704 CAATTG
SfaNI 454 GCATC GCATC(5/9)
472 GCATC
SspI 322 AATATT AAT^ATT
849 AATATT
TaqI 144 TCGA T^CGA
457 TCGA
Tsp45I 645 GTCAC ^GT[GC]AC
818 GTCAC
TspRI 647 CACTG CA[GC]TG[ACGT][ACGT]^
775 CAGTG
TthHB8I 144 TCGA T^CGA
457 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AluI BsmAI BssKI BstF5I BstNI
DdeI DpnII EcoRII FokI MaeIII
MboI MboII MnlI MvaI NdeII
NlaIII NlaIV Sau3AI ScrFI Tsp509I