Restriction map for
pF1KE4483
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BanI BbeI BbvCI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsiEI BsiWI BsmBI
BspDI BspHI BspLU11I BspMI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MluNI MscI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 1198 GTCTAC GT^[AC][GT]AC
AccII 668 CGCG CG^CG
AccIII 627 TCCGGA T^CCGGA
AcyI 343 GACGCC G[GA]^CG[CT]C
AflIII 667 ACGCGT A^C[GA][CT]GT
Alw44I 888 GTGCAC G^TGCAC
AlwNI 1138 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 586 GGGCCC GGGCC^C
ApaLI 888 GTGCAC G^TGCAC
BamHI 576 GGATCC G^GATCC
BbrPI 517 CACGTG CAC^GTG
BcgI 1170 CGAGCAGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 517 CACGTG [CT]AC^GT[GA]
BsaBI 1050 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 343 GACGCC G[GA]^CG[CT]C
BsaI 1220 GAGACC GGTCTC(1/5)
BsaWI 627 TCCGGA [AT]^CCGG[AT]
BseAI 627 TCCGGA T^CCGGA
BseRI 1207 GAGGAG GAGGAG(10/8)
BsmI 1176 GAATGC GAATGC(1/-1)
Bsp1407I 1387 TGTACA T^GTACA
BspEI 627 TCCGGA T^CCGGA
BsrDI 432 CATTGC GCAATG(2/0)
BsrGI 1387 TGTACA T^GTACA
BssSI 313 CTCGTG CACGAG(-5/-1)
BstUI 668 CGCG CG^CG
BstXI 413 CCAGGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoNI 1275 CCTGTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 167 CCAAGG C^C[AT][AT]GG
HgaI 343 GACGC GACGC(5/10)
Hin1I 343 GACGCC G[GA]^CG[CT]C
MamI 1050 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 779 CAATTG C^AATTG
MluI 667 ACGCGT A^CGCGT
MroI 627 TCCGGA T^CCGGA
MseI 152 TTAA T^TAA
MspA1I 1369 CAGCGG C[AC]G^C[GT]G
MunI 779 CAATTG C^AATTG
MvnI 668 CGCG CG^CG
NdeI 785 CATATG CA^TATG
NspI 1022 GCATGC [GA]CATG^[CT]
PflMI 748 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 517 CACGTG CAC^GTG
PmlI 517 CACGTG CAC^GTG
PpuMI 170 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 126 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1429 GAGCTC GAGCT^C
SmaI 1013 CCCGGG CCC^GGG
SmlI 681 CTTGAG C^T[CT][GA]AG
SphI 1022 GCATGC GCATG^C
SspBI 1387 TGTACA T^GTACA
StyI 167 CCAAGG C^C[AT][AT]GG
Tru9I 152 TTAA T^TAA
Van91I 748 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1013 CCCGGG C^CCGGG
XmaI 1013 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AspHI 888 GTGCAC G[AT]GC[AT]^C
1429 GAGCTC
AvaI 478 CTCGGG C^[CT]CG[GA]G
1013 CCCGGG
AvaII 76 GGTCC G^G[AT]CC
171 GGACC
BpmI 30 CTGGAG CTGGAG(16/14)
1458 CTGGAG
BsgI 304 CTGCAC GTGCAG(16/14)
325 CTGCAC
BsiHKAI 888 GTGCAC G[AT]GC[AT]^C
1429 GAGCTC
BsmAI 1220 GAGAC GTCTC(1/5)
1302 GTCTC
BsoBI 478 CTCGGG C^[CT]CG[GA]G
1013 CCCGGG
BsrBI 282 GAGCGG CCGCTC(-3/-3)
1162 GAGCGG
CfoI 514 GCGC GCG^C
1341 GCGC
DraII 170 AGGACCC [GA]G^G[ACGT]CC[CT]
1252 GGGGCCT
EaeI 204 CGGCCA [CT]^GGCC[GA]
521 TGGCCG
EcoO109I 170 AGGACCC [GA]G^G[ACGT]CC[CT]
1252 GGGGCCT
FauI 1310 CCCGC CCCGC(4/6)
55 GCGGG
HhaI 514 GCGC GCG^C
1341 GCGC
HinP1I 514 GCGC G^CGC
1341 GCGC
HincII 361 GTTGAC GT[CT]^[GA]AC
844 GTTGAC
HindII 361 GTTGAC GT[CT]^[GA]AC
844 GTTGAC
MaeII 518 ACGT A^CGT
1106 ACGT
MslI 798 CACAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1344 CATCCGGGTG
PstI 228 CTGCAG CTGCA^G
454 CTGCAG
TaqI 446 TCGA T^CGA
1169 TCGA
Tsp45I 737 GTGAC ^GT[GC]AC
1108 GTCAC
Tsp509I 780 AATT ^AATT
1089 AATT
TthHB8I 446 TCGA T^CGA
1169 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AlwI BanII
BbsI BbvI BcnI BmyI BpuAI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TfiI TseI TspRI
XcmI XhoII