Restriction map for pF1KE1823
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AfaI        AflII       AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BclI        BcnI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BseAI       BseRI       
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrDI       
BsrGI       BsrI        BssHII      BssSI       BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MslI        MunI        MvnI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     Van91I      XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI           37  GTATAC               GT^[AC][GT]AC
AciI          660  CCGC                 CCGC(-3/-1)
AflIII         95  ACATGT               A^C[GA][CT]GT
AgeI          916  ACCGGT               A^CCGGT
AlwI          501  GATCC                GGATC(4/5)
AspHI          44  GAGCTC               G[AT]GC[AT]^C
AvaI          648  CTCGGG               C^[CT]CG[GA]G
BanI          885  GGCACC               G^G[CT][GA]CC
BanII          44  GAGCTC               G[GA]GC[CT]^C
BglII         852  AGATCT               A^GATCT
BmyI           44  GAGCTC               G[AGT]GC[ACT]^C
BpmI          761  CTGGAG               CTGGAG(16/14)
BsaWI         916  ACCGGT               [AT]^CCGG[AT]
BsgI          478  GTGCAG               GTGCAG(16/14)
BsiHKAI        44  GAGCTC               G[AT]GC[AT]^C
BsmI          288  GAATGC               GAATGC(1/-1)
BsoBI         648  CTCGGG               C^[CT]CG[GA]G
Bsp1286I       44  GAGCTC               G[AGT]GC[ACT]^C
BspLU11I       95  ACATGT               A^CATGT
BsrFI         916  ACCGGT               [GA]^CCGG[CT]
Bst1107I       37  GTATAC               GTA^TAC
BstZ17I        37  GTATAC               GTA^TAC
CfoI          680  GCGC                 GCG^C
Cfr10I        916  ACCGGT               [GA]^CCGG[CT]
DraII         160  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO109I      160  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRI         246  GAATTC               G^AATTC
HapII         917  CCGG                 C^CGG
HgaI          614  GCGTC                GACGC(5/10)
HhaI          680  GCGC                 GCG^C
HinP1I        680  GCGC                 G^CGC
HpaII         917  CCGG                 C^CGG
MaeII         469  ACGT                 A^CGT
MspI          917  CCGG                 C^CGG
MwoI          590  GCAACTGCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI           95  ACATGT               [GA]CATG^[CT]
PinAI         916  ACCGGT               A^CCGGT
PpuMI         160  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          594  CTGCAG               CTGCA^G
PvuII         395  CAGCTG               CAG^CTG
SacI           44  GAGCTC               GAGCT^C
SapI           25  GCTCTTC              GCTCTTC(1/4)
SfaNI         128  GCATC                GCATC(5/9)
TaqI          518  TCGA                 T^CGA
TfiI          172  GATTC                G^A[AT]TC
TspRI         485  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       518  TCGA                 T^CGA
XbaI          102  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AcsI          246  GAATTC               [GA]^AATT[CT]
              555  AAATTT              
AluI           45  AGCT                 AG^CT
              396  AGCT                
ApoI          246  GAATTC               [GA]^AATT[CT]
              555  AAATTT              
AvaII         161  GGACC                G^G[AT]CC
              543  GGTCC               
BciVI         873  GGATAC               GTATCC(6/5)
              114  GTATCC              
BfaI          103  CTAG                 C^TAG
             1001  CTAG                
BsmAI          99  GTCTC                GTCTC(1/5)
              640  GTCTC               
BssKI         430  CCTGG                ^CC[ACGT]GG
              869  CCTGG               
BstF5I        624  CATCC                GGATG(2/0)
              942  GGATG               
BstNI         430  CCTGG                CC^[AT]GG
              869  CCTGG               
BstYI         500  AGATCC               [GA]^GATC[CT]
              852  AGATCT              
Cfr13I        161  GGACC                G^G[ACGT]CC
              543  GGTCC               
EcoRII        430  CCTGG                ^CC[AT]GG
              869  CCTGG               
EcoT14I       546  CCATGG               C^C[AT][AT]GG
              938  CCATGG              
FokI          624  CATCC                GGATG(9/13)
              942  GGATG               
HaeIII        473  GGCC                 GG^CC
              675  GGCC                
HincII        920  GTCAAC               GT[CT]^[GA]AC
              967  GTCAAC              
HindII        920  GTCAAC               GT[CT]^[GA]AC
              967  GTCAAC              
HphI          360  GGTGA                GGTGA(8/7)
              560  TCACC               
MaeI          103  CTAG                 C^TAG
             1001  CTAG                
MaeIII        442  GTCAC                ^GT[ACGT]AC
              634  GTCAC               
MflI          500  AGATCC               [GA]^GATC[CT]
              852  AGATCT              
MspA1I        395  CAGCTG               C[AC]G^C[GT]G
              660  CCGCTG              
MvaI          430  CCTGG                CC^[AT]GG
              869  CCTGG               
NcoI          546  CCATGG               C^CATGG
              938  CCATGG              
Sau96I        161  GGACC                G^G[ACGT]CC
              543  GGTCC               
ScrFI         430  CCTGG                CC^[ACGT]GG
              869  CCTGG               
StyI          546  CCATGG               C^C[AT][AT]GG
              938  CCATGG              
Tsp45I        442  GTCAC                ^GT[GC]AC
              634  GTCAC               
XcmI          340  CCACAGGGAACTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              929  CCAATCAATCCATGG     
XhoII         500  AGATCC               [GA]^GATC[CT]
              852  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

BbvI        BsaJI       BsiYI       BslI        BsmFI       
Cac8I       DdeI        DpnII       DsaI        EarI        
Fnu4HI      HinfI       ItaI        Ksp632I     MboI        
MboII       MnlI        MseI        NdeII       NlaIII      
NlaIV       PleI        Sau3AI      SfcI        Tru9I       
TseI        Tsp509I     
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