################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AfaI AflII AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrGI BsrI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI Bsu36I CelII ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 37 GTATAC GT^[AC][GT]AC AciI 660 CCGC CCGC(-3/-1) AflIII 95 ACATGT A^C[GA][CT]GT AgeI 916 ACCGGT A^CCGGT AlwI 501 GATCC GGATC(4/5) AspHI 44 GAGCTC G[AT]GC[AT]^C AvaI 648 CTCGGG C^[CT]CG[GA]G BanI 885 GGCACC G^G[CT][GA]CC BanII 44 GAGCTC G[GA]GC[CT]^C BglII 852 AGATCT A^GATCT BmyI 44 GAGCTC G[AGT]GC[ACT]^C BpmI 761 CTGGAG CTGGAG(16/14) BsaWI 916 ACCGGT [AT]^CCGG[AT] BsgI 478 GTGCAG GTGCAG(16/14) BsiHKAI 44 GAGCTC G[AT]GC[AT]^C BsmI 288 GAATGC GAATGC(1/-1) BsoBI 648 CTCGGG C^[CT]CG[GA]G Bsp1286I 44 GAGCTC G[AGT]GC[ACT]^C BspLU11I 95 ACATGT A^CATGT BsrFI 916 ACCGGT [GA]^CCGG[CT] Bst1107I 37 GTATAC GTA^TAC BstZ17I 37 GTATAC GTA^TAC CfoI 680 GCGC GCG^C Cfr10I 916 ACCGGT [GA]^CCGG[CT] DraII 160 GGGACCC [GA]G^G[ACGT]CC[CT] EcoO109I 160 GGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 246 GAATTC G^AATTC HapII 917 CCGG C^CGG HgaI 614 GCGTC GACGC(5/10) HhaI 680 GCGC GCG^C HinP1I 680 GCGC G^CGC HpaII 917 CCGG C^CGG MaeII 469 ACGT A^CGT MspI 917 CCGG C^CGG MwoI 590 GCAACTGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspI 95 ACATGT [GA]CATG^[CT] PinAI 916 ACCGGT A^CCGGT PpuMI 160 GGGACCC [GA]G^G[AT]CC[CT] PstI 594 CTGCAG CTGCA^G PvuII 395 CAGCTG CAG^CTG SacI 44 GAGCTC GAGCT^C SapI 25 GCTCTTC GCTCTTC(1/4) SfaNI 128 GCATC GCATC(5/9) TaqI 518 TCGA T^CGA TfiI 172 GATTC G^A[AT]TC TspRI 485 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 518 TCGA T^CGA XbaI 102 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcsI 246 GAATTC [GA]^AATT[CT] 555 AAATTT AluI 45 AGCT AG^CT 396 AGCT ApoI 246 GAATTC [GA]^AATT[CT] 555 AAATTT AvaII 161 GGACC G^G[AT]CC 543 GGTCC BciVI 873 GGATAC GTATCC(6/5) 114 GTATCC BfaI 103 CTAG C^TAG 1001 CTAG BsmAI 99 GTCTC GTCTC(1/5) 640 GTCTC BssKI 430 CCTGG ^CC[ACGT]GG 869 CCTGG BstF5I 624 CATCC GGATG(2/0) 942 GGATG BstNI 430 CCTGG CC^[AT]GG 869 CCTGG BstYI 500 AGATCC [GA]^GATC[CT] 852 AGATCT Cfr13I 161 GGACC G^G[ACGT]CC 543 GGTCC EcoRII 430 CCTGG ^CC[AT]GG 869 CCTGG EcoT14I 546 CCATGG C^C[AT][AT]GG 938 CCATGG FokI 624 CATCC GGATG(9/13) 942 GGATG HaeIII 473 GGCC GG^CC 675 GGCC HincII 920 GTCAAC GT[CT]^[GA]AC 967 GTCAAC HindII 920 GTCAAC GT[CT]^[GA]AC 967 GTCAAC HphI 360 GGTGA GGTGA(8/7) 560 TCACC MaeI 103 CTAG C^TAG 1001 CTAG MaeIII 442 GTCAC ^GT[ACGT]AC 634 GTCAC MflI 500 AGATCC [GA]^GATC[CT] 852 AGATCT MspA1I 395 CAGCTG C[AC]G^C[GT]G 660 CCGCTG MvaI 430 CCTGG CC^[AT]GG 869 CCTGG NcoI 546 CCATGG C^CATGG 938 CCATGG Sau96I 161 GGACC G^G[ACGT]CC 543 GGTCC ScrFI 430 CCTGG CC^[ACGT]GG 869 CCTGG StyI 546 CCATGG C^C[AT][AT]GG 938 CCATGG Tsp45I 442 GTCAC ^GT[GC]AC 634 GTCAC XcmI 340 CCACAGGGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 929 CCAATCAATCCATGG XhoII 500 AGATCC [GA]^GATC[CT] 852 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### BbvI BsaJI BsiYI BslI BsmFI Cac8I DdeI DpnII DsaI EarI Fnu4HI HinfI ItaI Ksp632I MboI MboII MnlI MseI NdeII NlaIII NlaIV PleI Sau3AI SfcI Tru9I TseI Tsp509I