Restriction map for
pF1KE2728
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BbrPI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaBI BseAI
BsiHKAI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BssHII Bst1107I BstAPI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI Eam1105I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI TfiI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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BalI 1739 TGGCCA TGG^CCA
BfaI 1345 CTAG C^TAG
BglII 1002 AGATCT A^GATCT
BsaAI 805 TACGTG [CT]AC^GT[GA]
BsaWI 511 ACCGGA [AT]^CCGG[AT]
BsiEI 206 CGGCCG CG[GA][CT]^CG
Bsp1407I 428 TGTACA T^GTACA
BsrGI 428 TGTACA T^GTACA
BssSI 1147 CTCGTG CACGAG(-5/-1)
BstBI 997 TTCGAA TT^CGAA
BstYI 1002 AGATCT [GA]^GATC[CT]
DraIII 620 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 206 CGGCCG C^GGCCG
EarI 310 CTCTTC CTCTTC(1/4)
EclXI 206 CGGCCG C^GGCCG
Eco52I 206 CGGCCG C^GGCCG
HincII 1462 GTCAAC GT[CT]^[GA]AC
HindII 1462 GTCAAC GT[CT]^[GA]AC
Ksp632I 310 CTCTTC CTCTTC(1/4)
MaeI 1345 CTAG C^TAG
MflI 1002 AGATCT [GA]^GATC[CT]
MluNI 1739 TGGCCA TGG^CCA
MscI 1739 TGGCCA TGG^CCA
MseI 701 TTAA T^TAA
NspI 971 ACATGC [GA]CATG^[CT]
NspV 997 TTCGAA TT^CGAA
PflMI 579 CCAGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1088 CTGCAG CTGCA^G
PvuII 1638 CAGCTG CAG^CTG
SapI 309 GCTCTTC GCTCTTC(1/4)
SexAI 854 ACCAGGT A^CC[AT]GGT
SfiI 831 GGCCAAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 997 TTCGAA TT^CGAA
SmlI 253 CTCAAG C^T[CT][GA]AG
SspBI 428 TGTACA T^GTACA
StuI 236 AGGCCT AGG^CCT
Tru9I 701 TTAA T^TAA
Tsp509I 516 AATT ^AATT
Van91I 579 CCAGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1002 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
ApaI 643 GGGCCC GGGCC^C
1517 GGGCCC
BbeI 155 GGCGCC GGCGC^C
1384 GGCGCC
BbsI 1102 GAAGAC GAAGAC(2/6)
322 GTCTTC
BbvCI 1053 CCTCAGC CCTCAGC(-5/-2)
130 GCTGAGG
BglI 832 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1643 GCCTGACAGGC
BpuAI 1102 GAAGAC GAAGAC(2/6)
322 GTCTTC
BsaI 13 GAGACC GGTCTC(1/5)
1907 GAGACC
BspMI 213 GCAGGT ACCTGC(4/8)
792 GCAGGT
DrdI 46 GACTACGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1753 GACAACTTGGTC
EaeI 206 CGGCCG [CT]^GGCC[GA]
1739 TGGCCA
KasI 155 GGCGCC G^GCGCC
1384 GGCGCC
MaeII 754 ACGT A^CGT
806 ACGT
MaeIII 475 GTGAC ^GT[ACGT]AC
1093 GTCAC
NaeI 152 GCCGGC GCC^GGC
1311 GCCGGC
NarI 155 GGCGCC GG^CGCC
1384 GGCGCC
NgoMIV 152 GCCGGC G^CCGGC
1311 GCCGGC
SfcI 1088 CTGCAG C^T[GA][CT]AG
1097 CTGTAG
SfoI 155 GGCGCC GGC^GCC
1384 GGCGCC
SmaI 1018 CCCGGG CCC^GGG
1677 CCCGGG
TaqI 998 TCGA T^CGA
1905 TCGA
Tsp45I 475 GTGAC ^GT[GC]AC
1093 GTCAC
TthHB8I 998 TCGA T^CGA
1905 TCGA
XcmI 1523 CCAGCTCAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1727 CCAAGGAAGAAGTGG
XmaCI 1018 CCCGGG C^CCGGG
1677 CCCGGG
XmaI 1018 CCCGGG C^CCGGG
1677 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
Eco47III EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TseI
TspRI