Restriction map for
pF1KB5830
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BcgI
BciVI BfaI BglI BglII BlnI
BsaBI BsaI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstPI Cfr10I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HgaI HindIII
HpaI KpnI KspI MaeI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcsI 1324 GAATTT [GA]^AATT[CT]
AcyI 951 GGCGCC G[GA]^CG[CT]C
AflII 1153 CTTAAG C^TTAAG
ApoI 1324 GAATTT [GA]^AATT[CT]
AspI 1246 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 858 TGGCCA TGG^CCA
BbeI 951 GGCGCC GGCGC^C
BbrPI 265 CACGTG CAC^GTG
BbvCI 1227 CCTCAGC CCTCAGC(-5/-2)
BclI 135 TGATCA T^GATCA
BfrI 1153 CTTAAG C^TTAAG
BlpI 1116 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1116 GCTCAGC GC^T[ACGT]AGC
BsaAI 265 CACGTG [CT]AC^GT[GA]
BsaHI 951 GGCGCC G[GA]^CG[CT]C
BsgI 767 GTGCAG GTGCAG(16/14)
BsmAI 778 GTCTC GTCTC(1/5)
BsmI 175 GAATGC GAATGC(1/-1)
BsrBI 748 GAGCGG CCGCTC(-3/-3)
BsrDI 564 CATTGC GCAATG(2/0)
BssSI 1063 CACGAG CACGAG(-5/-1)
Bst1107I 814 GTATAC GTA^TAC
BstZ17I 814 GTATAC GTA^TAC
CelII 1116 GCTCAGC GC^T[ACGT]AGC
EagI 1035 CGGCCG C^GGCCG
EclXI 1035 CGGCCG C^GGCCG
Eco52I 1035 CGGCCG C^GGCCG
FbaI 135 TGATCA T^GATCA
Hin1I 951 GGCGCC G[GA]^CG[CT]C
HincII 1300 GTCGAC GT[CT]^[GA]AC
HindII 1300 GTCGAC GT[CT]^[GA]AC
KasI 951 GGCGCC G^GCGCC
MluNI 858 TGGCCA TGG^CCA
MscI 858 TGGCCA TGG^CCA
NarI 951 GGCGCC GG^CGCC
NcoI 356 CCATGG C^CATGG
NotI 1034 GCGGCCGC GC^GGCCGC
PflFI 1246 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 265 CACGTG CAC^GTG
PmlI 265 CACGTG CAC^GTG
PshAI 240 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 232 CTGCAG CTGCA^G
PvuII 181 CAGCTG CAG^CTG
SacI 1078 GAGCTC GAGCT^C
SalI 1300 GTCGAC G^TCGAC
SapI 87 GAAGAGC GCTCTTC(1/4)
ScaI 791 AGTACT AGT^ACT
SfcI 232 CTGCAG C^T[GA][CT]AG
SfoI 951 GGCGCC GGC^GCC
SmaI 725 CCCGGG CCC^GGG
TfiI 271 GATTC G^A[AT]TC
Tsp509I 1325 AATT ^AATT
Tth111I 1246 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1199 CCAAGGAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 725 CCCGGG C^CCGGG
XmaI 725 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 814 GTATAC GT^[AC][GT]AC
1300 GTCGAC
AlwI 543 GATCC GGATC(4/5)
1098 GATCC
AocI 955 CCTGAGG CC^T[ACGT]AGG
1282 CCTCAGG
Asp700I 447 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
921 GAAAACCTTC
AspHI 1078 GAGCTC G[AT]GC[AT]^C
1213 GAGCAC
BanI 623 GGCACC G^G[CT][GA]CC
951 GGCGCC
BbsI 189 GAAGAC GAAGAC(2/6)
736 GTCTTC
BpuAI 189 GAAGAC GAAGAC(2/6)
736 GTCTTC
BsiEI 666 CGACCG CG[GA][CT]^CG
1035 CGGCCG
BsiHKAI 1078 GAGCTC G[AT]GC[AT]^C
1213 GAGCAC
BstXI 36 CCACAAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
305 CCATCCAGATGG
BstYI 542 AGATCC [GA]^GATC[CT]
1097 AGATCC
Bsu36I 955 CCTGAGG CC^T[ACGT]AGG
1282 CCTCAGG
Eco81I 955 CCTGAGG CC^T[ACGT]AGG
1282 CCTCAGG
EcoNI 471 CCTTGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
546 CCTGCGGAAGG
HaeII 951 GGCGCC [GA]GCGC^[CT]
1067 AGCGCC
MflI 542 AGATCC [GA]^GATC[CT]
1097 AGATCC
MseI 154 TTAA T^TAA
1154 TTAA
PleI 141 GACTC GAGTC(4/5)
385 GACTC
Tru9I 154 TTAA T^TAA
1154 TTAA
XhoII 542 AGATCC [GA]^GATC[CT]
1097 AGATCC
XmnI 447 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
921 GAAAACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AfaI AluI ApaI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI TseI Tsp45I TspRI
TthHB8I