Restriction map for
pF1KE3316
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AscI AseI AsnI Asp718I AspI
AvrII BamHI BbrPI BciVI BfrI
BlnI BsaAI BsaBI BsiEI BsiWI
Bsp1407I BspDI BspHI BsrGI BssSI
BstBI BstEII BstPI ClaI CpoI
DraI EagI EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI HincII
HindII HpaI KpnI KspI MamI
MfeI MluI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcsI 1319 AAATTC [GA]^AATT[CT]
Alw44I 427 GTGCAC G^TGCAC
AocI 2065 CCTCAGG CC^T[ACGT]AGG
Aor51HI 533 AGCGCT AGC^GCT
ApaI 569 GGGCCC GGGCC^C
ApaLI 427 GTGCAC G^TGCAC
ApoI 1319 AAATTC [GA]^AATT[CT]
Asp700I 2112 GAAGTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2375 TGCGCA TGC^GCA
BbsI 1713 GAAGAC GAAGAC(2/6)
BcgI 429 GCACTCCTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1649 AGATCT A^GATCT
BlpI 663 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 663 GCTGAGC GC^T[ACGT]AGC
BpuAI 1713 GAAGAC GAAGAC(2/6)
BsgI 648 CTGCAC GTGCAG(16/14)
BsmI 1068 GAATGC GAATGC(1/-1)
BssHII 2503 GCGCGC G^CGCGC
Bst1107I 735 GTATAC GTA^TAC
BstZ17I 735 GTATAC GTA^TAC
Bsu36I 2065 CCTCAGG CC^T[ACGT]AGG
CelII 663 GCTGAGC GC^T[ACGT]AGC
DraIII 613 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1036 GACTCCGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2555 CCACGG C^C[GA][CT]GG
Eco47III 533 AGCGCT AGC^GCT
Eco81I 2065 CCTCAGG CC^T[ACGT]AGG
EcoT14I 776 CCAAGG C^C[AT][AT]GG
FspI 2375 TGCGCA TGC^GCA
HindIII 1580 AAGCTT A^AGCTT
SacI 1119 GAGCTC GAGCT^C
SexAI 356 ACCTGGT A^CC[AT]GGT
StyI 776 CCAAGG C^C[AT][AT]GG
Tsp509I 1320 AATT ^AATT
XmnI 2112 GAAGTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 34 enzymes) #
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AccI 735 GTATAC GT^[AC][GT]AC
1642 GTCTAC
AccIII 334 TCCGGA T^CCGGA
1784 TCCGGA
AhdI 295 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2197 GACATGTGGTC
AspEI 295 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2197 GACATGTGGTC
AspHI 427 GTGCAC G[AT]GC[AT]^C
1119 GAGCTC
BbvCI 2232 CCTCAGC CCTCAGC(-5/-2)
1480 GCTGAGG
BclI 456 TGATCA T^GATCA
2216 TGATCA
BfaI 76 CTAG C^TAG
484 CTAG
BsaI 53 GGTCTC GGTCTC(1/5)
132 GGTCTC
BseAI 334 TCCGGA T^CCGGA
1784 TCCGGA
BsiHKAI 427 GTGCAC G[AT]GC[AT]^C
1119 GAGCTC
BsmBI 596 CGTCTC CGTCTC(1/5)
1852 GAGACG
BspEI 334 TCCGGA T^CCGGA
1784 TCCGGA
BspLU11I 1898 ACATGT A^CATGT
2198 ACATGT
BspMI 2357 ACCTGC ACCTGC(4/8)
2364 GCAGGT
BsrBI 2124 CCGCTC CCGCTC(-3/-3)
2187 CCGCTC
BsrDI 1632 CATTGC GCAATG(2/0)
879 GCAATG
BsrFI 1931 GCCGGC [GA]^CCGG[CT]
2142 GCCGGC
BstXI 593 CCACGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
749 CCATTGAGCTGG
Cfr10I 1931 GCCGGC [GA]^CCGG[CT]
2142 GCCGGC
Eam1105I 295 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2197 GACATGTGGTC
FbaI 456 TGATCA T^GATCA
2216 TGATCA
MaeI 76 CTAG C^TAG
484 CTAG
MroI 334 TCCGGA T^CCGGA
1784 TCCGGA
MseI 899 TTAA T^TAA
1736 TTAA
NaeI 1931 GCCGGC GCC^GGC
2142 GCCGGC
NgoMIV 1931 GCCGGC G^CCGGC
2142 GCCGGC
PflMI 1968 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2309 CCAGCCCCTGG
SmlI 499 CTCAAG C^T[CT][GA]AG
853 CTCAAG
TaqI 392 TCGA T^CGA
1850 TCGA
Tru9I 899 TTAA T^TAA
1736 TTAA
TthHB8I 392 TCGA T^CGA
1850 TCGA
Van91I 1968 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2309 CCAGCCCCTGG
XcmI 1421 CCACCTACTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2322 CCACATCTCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AvaI AvaII
BalI BanI BanII BbeI BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BsaWI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoNI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
TseI Tsp45I TspRI XhoII XmaCI
XmaI