Restriction map for
pF1KE2344
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# NO CUT ENZYMES ( 64 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AflIII AgeI AocI ApoI AseI
AsnI Asp700I Asp718I BamHI BclI
BfrI BglII BsaBI BseAI BsmBI
BsmI BspEI BspLU11I Bst1107I BstAPI
BstZ17I Bsu36I DraI Eco81I EcoRI
EcoRV EcoT22I FbaI FseI HpaI
KpnI MamI MfeI MluI MroI
MseI MunI NdeI NheI NruI
NsiI PacI PinAI PmeI PshBI
Psp1406I PvuI SbfI ScaI SnaBI
SpeI SphI Sse8387I SspI SwaI
TfiI Tru9I XbaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 552 GACGTC GACGT^C
AccI 3463 GTCGAC GT^[AC][GT]AC
AspI 1372 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 230 TGCGCA TGC^GCA
AvrII 2916 CCTAGG C^CTAGG
BbrPI 1101 CACGTG CAC^GTG
BciVI 2061 GGATAC GTATCC(6/5)
BlnI 2916 CCTAGG C^CTAGG
BlpI 2989 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2989 GCTCAGC GC^T[ACGT]AGC
BsaI 1672 GAGACC GGTCTC(1/5)
BsgI 2377 CTGCAC GTGCAG(16/14)
Bsp1407I 2678 TGTACA T^GTACA
BspDI 1549 ATCGAT AT^CGAT
BsrDI 1703 GCAATG GCAATG(2/0)
BsrGI 2678 TGTACA T^GTACA
BstBI 349 TTCGAA TT^CGAA
BstEII 1521 GGTCACC G^GT[ACGT]ACC
BstPI 1521 GGTCACC G^GT[ACGT]ACC
CelII 2989 GCTCAGC GC^T[ACGT]AGC
ClaI 1549 ATCGAT AT^CGAT
CpoI 3519 CGGTCCG CG^G[AT]CCG
DrdI 1651 GACTTCTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1521 GGTCACC G^GT[ACGT]ACC
FspI 230 TGCGCA TGC^GCA
HincII 3463 GTCGAC GT[CT]^[GA]AC
HindII 3463 GTCGAC GT[CT]^[GA]AC
HindIII 2320 AAGCTT A^AGCTT
MslI 609 CATTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 2564 CCATGG C^CATGG
NotI 144 GCGGCCGC GC^GGCCGC
NspV 349 TTCGAA TT^CGAA
PflFI 1372 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2640 CCACCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1101 CACGTG CAC^GTG
PmlI 1101 CACGTG CAC^GTG
PshAI 625 GACGGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 3519 CGGTCCG CG^G[AT]CCG
SacI 1027 GAGCTC GAGCT^C
SalI 3463 GTCGAC G^TCGAC
SfuI 349 TTCGAA TT^CGAA
SspBI 2678 TGTACA T^GTACA
StuI 2919 AGGCCT AGG^CCT
Tth111I 1372 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2640 CCACCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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Alw44I 334 GTGCAC G^TGCAC
430 GTGCAC
ApaLI 334 GTGCAC G^TGCAC
430 GTGCAC
AscI 3482 GGCGCGCC GG^CGCGCC
3761 GGCGCGCC
BbvCI 3822 CCTCAGC CCTCAGC(-5/-2)
778 GCTGAGG
BcgI 916 GCAGTGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2342 GCAAGGTCTTCG
BsaAI 1101 CACGTG [CT]AC^GT[GA]
2056 TACGTG
BsaWI 1419 ACCGGA [AT]^CCGG[AT]
3935 ACCGGA
BsiWI 182 CGTACG C^GTACG
3030 CGTACG
BspHI 1091 TCATGA T^CATGA
2039 TCATGA
BstXI 831 CCAGCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2405 CCATCGACCTGG
DraIII 337 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
2599 CACCTGGTG
EcoNI 2376 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2663 CCTTCTCCAGG
RcaI 1091 TCATGA T^CATGA
2039 TCATGA
SapI 494 GCTCTTC GCTCTTC(1/4)
735 GCTCTTC
SexAI 1517 ACCTGGT A^CC[AT]GGT
2600 ACCTGGT
Tsp509I 1927 AATT ^AATT
2290 AATT
XcmI 1961 CCAGCAAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2636 CCACCCACCGCATGG
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# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI Aor51HI ApaI
AspEI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssHII BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PaeR7I PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SfoI SgrAI
SmaI SmlI StyI TaqI TseI
Tsp45I TspRI TthHB8I XhoI XhoII
XmaCI XmaI