Restriction map for
pF1KE2515
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI AlwNI AocI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaBI BsaI BseAI BsgI BsiEI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII Bst1107I BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FbaI FseI HgaI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TfiI Tth111I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 280 GACGTC GACGT^C
AccII 18 CGCG CG^CG
AcsI 36 GAATTT [GA]^AATT[CT]
Alw44I 841 GTGCAC G^TGCAC
ApaI 329 GGGCCC GGGCC^C
ApaLI 841 GTGCAC G^TGCAC
ApoI 36 GAATTT [GA]^AATT[CT]
AviII 212 TGCGCA TGC^GCA
BbeI 555 GGCGCC GGCGC^C
BbrPI 409 CACGTG CAC^GTG
BbvCI 130 GCTGAGG CCTCAGC(-5/-2)
BcgI 335 GCAAGGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 706 CTAG C^TAG
BglII 989 AGATCT A^GATCT
BpmI 70 CTCCAG CTGGAG(16/14)
BsaWI 1103 ACCGGA [AT]^CCGG[AT]
BspLU11I 668 ACATGT A^CATGT
BssSI 187 CACGAG CACGAG(-5/-1)
BstAPI 299 GCATCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 18 CGCG CG^CG
DdeI 131 CTGAG C^T[ACGT]AG
DraIII 409 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
FauI 332 CCCGC CCCGC(4/6)
FspI 212 TGCGCA TGC^GCA
HaeII 555 GGCGCC [GA]GCGC^[CT]
KasI 555 GGCGCC G^GCGCC
MaeI 706 CTAG C^TAG
MseI 695 TTAA T^TAA
MslI 1002 CATCGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 18 CGCG CG^CG
NarI 555 GGCGCC GG^CGCC
PmaCI 409 CACGTG CAC^GTG
PmlI 409 CACGTG CAC^GTG
PpuMI 1034 GGGACCT [GA]G^G[AT]CC[CT]
SacI 448 GAGCTC GAGCT^C
SfoI 555 GGCGCC GGC^GCC
SmaI 539 CCCGGG CCC^GGG
SphI 1007 GCATGC GCATG^C
Tru9I 695 TTAA T^TAA
Tsp509I 37 AATT ^AATT
XmaCI 539 CCCGGG C^CCGGG
XmaI 539 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 280 GACGTC G[GA]^CG[CT]C
555 GGCGCC
AfaI 621 GTAC GT^AC
924 GTAC
AflIII 653 ACGTGT A^C[GA][CT]GT
668 ACATGT
AlwI 636 GATCC GGATC(4/5)
347 GGATC
BalI 99 TGGCCA TGG^CCA
689 TGGCCA
BbsI 787 GAAGAC GAAGAC(2/6)
975 GAAGAC
BglI 46 GCCACCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
847 GCCCTGGTGGC
BpuAI 787 GAAGAC GAAGAC(2/6)
975 GAAGAC
BsaAI 409 CACGTG [CT]AC^GT[GA]
838 TACGTG
BsaHI 280 GACGTC G[GA]^CG[CT]C
555 GGCGCC
BstEII 414 GGTGACC G^GT[ACGT]ACC
735 GGTCACC
BstPI 414 GGTGACC G^GT[ACGT]ACC
735 GGTCACC
BstXI 725 CCAAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1049 CCATCGACCTGG
BstYI 635 AGATCC [GA]^GATC[CT]
989 AGATCT
DraII 328 GGGGCCC [GA]G^G[ACGT]CC[CT]
1034 GGGACCT
EarI 256 CTCTTC CTCTTC(1/4)
502 CTCTTC
EcoNI 255 CCTCTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
750 CCTCATAAAGG
EcoO109I 328 GGGGCCC [GA]G^G[ACGT]CC[CT]
1034 GGGACCT
EcoO65I 414 GGTGACC G^GT[ACGT]ACC
735 GGTCACC
EcoT14I 50 CCTTGG C^C[AT][AT]GG
320 CCAAGG
Hin1I 280 GACGTC G[GA]^CG[CT]C
555 GGCGCC
Ksp632I 256 CTCTTC CTCTTC(1/4)
502 CTCTTC
MflI 635 AGATCC [GA]^GATC[CT]
989 AGATCT
MluNI 99 TGGCCA TGG^CCA
689 TGGCCA
MscI 99 TGGCCA TGG^CCA
689 TGGCCA
MspA1I 730 CAGCTG C[AC]G^C[GT]G
1072 CAGCTG
PvuII 730 CAGCTG CAG^CTG
1072 CAGCTG
RsaI 621 GTAC GT^AC
924 GTAC
StyI 50 CCTTGG C^C[AT][AT]GG
320 CCAAGG
XhoII 635 AGATCC [GA]^GATC[CT]
989 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AluI AspHI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI Sau3AI
Sau96I ScrFI SfaNI TaqI TseI
Tsp45I TspRI TthHB8I