Restriction map for
pF1KE4235
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AccI AccIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AvrII BcgI
BclI BlnI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspHI Bst1107I BstBI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRV EcoT22I FbaI FseI
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshBI PstI PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI XbaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 2518 GACGTC GACGT^C
AclI 1573 AACGTT AA^CGTT
AcsI 908 GAATTC [GA]^AATT[CT]
AocI 1795 CCTGAGG CC^T[ACGT]AGG
ApaI 777 GGGCCC GGGCC^C
ApoI 908 GAATTC [GA]^AATT[CT]
Asp700I 765 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1479 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1528 GGATCC G^GATCC
BbeI 268 GGCGCC GGCGC^C
BglI 260 GCCACCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 310 CTGCAC GTGCAG(16/14)
Bsp1407I 1595 TGTACA T^GTACA
BspLU11I 1592 ACATGT A^CATGT
BspMI 731 ACCTGC ACCTGC(4/8)
BsrFI 3 GCCGGC [GA]^CCGG[CT]
BsrGI 1595 TGTACA T^GTACA
BssHII 332 GCGCGC G^CGCGC
BstAPI 77 GCAAGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2035 GGTGACC G^GT[ACGT]ACC
BstPI 2035 GGTGACC G^GT[ACGT]ACC
Bsu36I 1795 CCTGAGG CC^T[ACGT]AGG
Cfr10I 3 GCCGGC [GA]^CCGG[CT]
Eco81I 1795 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2035 GGTGACC G^GT[ACGT]ACC
EcoRI 908 GAATTC G^AATTC
HaeII 268 GGCGCC [GA]GCGC^[CT]
HgaI 2269 GACGC GACGC(5/10)
HincII 2020 GTCAAC GT[CT]^[GA]AC
HindII 2020 GTCAAC GT[CT]^[GA]AC
HindIII 925 AAGCTT A^AGCTT
KasI 268 GGCGCC G^GCGCC
NaeI 3 GCCGGC GCC^GGC
NarI 268 GGCGCC GG^CGCC
NgoMIV 3 GCCGGC G^CCGGC
PaeR7I 208 CTCGAG C^TCGAG
PflFI 1479 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 527 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 2038 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1573 AACGTT AA^CGTT
SapI 1216 GCTCTTC GCTCTTC(1/4)
ScaI 245 AGTACT AGT^ACT
SfoI 268 GGCGCC GGC^GCC
SmaI 264 CCCGGG CCC^GGG
SspBI 1595 TGTACA T^GTACA
Tth111I 1479 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 208 CTCGAG C^TCGAG
XmaCI 264 CCCGGG C^CCGGG
XmaI 264 CCCGGG C^CCGGG
XmnI 765 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 268 GGCGCC G[GA]^CG[CT]C
2518 GACGTC
AflII 922 CTTAAG C^TTAAG
2424 CTTAAG
AflIII 494 ACACGT A^C[GA][CT]GT
1592 ACATGT
AlwNI 952 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1564 CAGCACCTG
AviII 71 TGCGCA TGC^GCA
502 TGCGCA
BalI 803 TGGCCA TGG^CCA
1607 TGGCCA
BanI 268 GGCGCC G^G[CT][GA]CC
1891 GGTGCC
BbrPI 495 CACGTG CAC^GTG
691 CACGTG
BbvCI 1561 CCTCAGC CCTCAGC(-5/-2)
735 GCTGAGG
BciVI 1336 GTATCC GTATCC(6/5)
1691 GTATCC
BfrI 922 CTTAAG C^TTAAG
2424 CTTAAG
BglII 377 AGATCT A^GATCT
890 AGATCT
BlpI 336 GCTCAGC GC^T[ACGT]AGC
2154 GCTGAGC
Bpu1102I 336 GCTCAGC GC^T[ACGT]AGC
2154 GCTGAGC
BsaAI 495 CACGTG [CT]AC^GT[GA]
691 CACGTG
BsaHI 268 GGCGCC G[GA]^CG[CT]C
2518 GACGTC
BsaI 457 GAGACC GGTCTC(1/5)
2207 GGTCTC
BsrBI 30 CCGCTC CCGCTC(-3/-3)
469 GAGCGG
BsrDI 609 CATTGC GCAATG(2/0)
1194 CATTGC
BssSI 124 CACGAG CACGAG(-5/-1)
2558 CACGAG
BstXI 936 CCACTCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2132 CCACGCTTTTGG
CelII 336 GCTCAGC GC^T[ACGT]AGC
2154 GCTGAGC
FspI 71 TGCGCA TGC^GCA
502 TGCGCA
Hin1I 268 GGCGCC G[GA]^CG[CT]C
2518 GACGTC
MaeIII 823 GTCAC ^GT[ACGT]AC
2036 GTGAC
MluNI 803 TGGCCA TGG^CCA
1607 TGGCCA
MscI 803 TGGCCA TGG^CCA
1607 TGGCCA
MspA1I 682 CAGCTG C[AC]G^C[GT]G
2293 CAGCTG
NspI 1592 ACATGT [GA]CATG^[CT]
1742 ACATGC
PflMI 755 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
951 CCAGTCTCTGG
PmaCI 495 CACGTG CAC^GTG
691 CACGTG
PmlI 495 CACGTG CAC^GTG
691 CACGTG
PvuII 682 CAGCTG CAG^CTG
2293 CAGCTG
TfiI 841 GAATC G^A[AT]TC
1808 GATTC
Tsp45I 823 GTCAC ^GT[GC]AC
2036 GTGAC
Van91I 755 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
951 CCAGTCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I XcmI XhoII