Restriction map for
pF1KE1498
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# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AccIII AflII AscI AseI
AsnI AvrII BbrPI BcgI BfrI
BlnI BsaBI BseAI BsiWI BspDI
BspEI BspHI BsrDI BssHII Bst1107I
BstEII BstPI BstZ17I ClaI CpoI
EagI EclXI Eco52I EcoO65I EcoRV
EcoT22I HpaI MamI MfeI MroI
MunI NdeI NheI NotI NsiI
PacI PmaCI PmeI PmlI PshBI
PvuI RcaI RsrII ScaI SexAI
SgrAI SmaI SnaBI SpeI SwaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AclI 2242 AACGTT AA^CGTT
AgeI 2641 ACCGGT A^CCGGT
AocI 3061 CCTCAGG CC^T[ACGT]AGG
Aor51HI 4014 AGCGCT AGC^GCT
ApaI 466 GGGCCC GGGCC^C
AspI 1534 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3575 GGATCC G^GATCC
BbeI 1312 GGCGCC GGCGC^C
BciVI 2097 GGATAC GTATCC(6/5)
BlpI 666 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 666 GCTCAGC GC^T[ACGT]AGC
BsaWI 2641 ACCGGT [AT]^CCGG[AT]
Bsp1407I 2495 TGTACA T^GTACA
BspLU11I 21 ACATGT A^CATGT
BsrGI 2495 TGTACA T^GTACA
BstBI 2609 TTCGAA TT^CGAA
Bsu36I 3061 CCTCAGG CC^T[ACGT]AGG
CelII 666 GCTCAGC GC^T[ACGT]AGC
DraI 3174 TTTAAA TTT^AAA
DraIII 2824 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4014 AGCGCT AGC^GCT
Eco81I 3061 CCTCAGG CC^T[ACGT]AGG
FseI 4286 GGCCGGCC GGCCGG^CC
HindIII 3340 AAGCTT A^AGCTT
KasI 1312 GGCGCC G^GCGCC
KspI 658 CCGCGG CCGC^GG
MluI 4637 ACGCGT A^CGCGT
NarI 1312 GGCGCC GG^CGCC
NcoI 1949 CCATGG C^CATGG
NruI 1649 TCGCGA TCG^CGA
NspV 2609 TTCGAA TT^CGAA
PaeR7I 1831 CTCGAG C^TCGAG
PflFI 1534 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2641 ACCGGT A^CCGGT
Psp1406I 2242 AACGTT AA^CGTT
SacII 658 CCGCGG CCGC^GG
SalI 1931 GTCGAC G^TCGAC
SapI 944 GAAGAGC GCTCTTC(1/4)
SbfI 4569 CCTGCAGG CCTGCA^GG
SfiI 4281 GGCCAGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1312 GGCGCC GGC^GCC
SfuI 2609 TTCGAA TT^CGAA
Sse8387I 4569 CCTGCAGG CCTGCA^GG
SspBI 2495 TGTACA T^GTACA
Tth111I 1534 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3303 TCTAGA T^CTAGA
XcmI 3711 CCATGAAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1831 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 38 enzymes) #
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Acc65I 238 GGTACC G^GTACC
3324 GGTACC
AccI 334 GTAGAC GT^[AC][GT]AC
1931 GTCGAC
AcyI 1312 GGCGCC G[GA]^CG[CT]C
2710 GACGCC
AflIII 21 ACATGT A^C[GA][CT]GT
4637 ACGCGT
AhdI 1030 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1438 GACGGCAGGTC
Alw44I 1339 GTGCAC G^TGCAC
4268 GTGCAC
ApaLI 1339 GTGCAC G^TGCAC
4268 GTGCAC
Asp700I 1964 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3217 GAAACTTTTC
Asp718I 238 GGTACC G^GTACC
3324 GGTACC
AspEI 1030 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1438 GACGGCAGGTC
AviII 1301 TGCGCA TGC^GCA
3122 TGCGCA
BalI 1946 TGGCCA TGG^CCA
3656 TGGCCA
BclI 2976 TGATCA T^GATCA
3354 TGATCA
BglII 1808 AGATCT A^GATCT
3780 AGATCT
BsaAI 2620 TACGTG [CT]AC^GT[GA]
3163 TACGTG
BsaHI 1312 GGCGCC G[GA]^CG[CT]C
2710 GACGCC
BsiEI 1646 CGGTCG CG[GA][CT]^CG
2336 CGGTCG
BssSI 31 CACGAG CACGAG(-5/-1)
2281 CACGAG
DrdI 313 GACTCTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2269 GACATTTGGGTC
Eam1105I 1030 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1438 GACGGCAGGTC
EcoNI 1200 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4246 CCTTTTGGAGG
EcoRI 544 GAATTC G^AATTC
3289 GAATTC
FbaI 2976 TGATCA T^GATCA
3354 TGATCA
FspI 1301 TGCGCA TGC^GCA
3122 TGCGCA
HaeII 1312 GGCGCC [GA]GCGC^[CT]
4014 AGCGCT
Hin1I 1312 GGCGCC G[GA]^CG[CT]C
2710 GACGCC
HincII 17 GTCAAC GT[CT]^[GA]AC
1931 GTCGAC
HindII 17 GTCAAC GT[CT]^[GA]AC
1931 GTCGAC
KpnI 238 GGTACC GGTAC^C
3324 GGTACC
MluNI 1946 TGGCCA TGG^CCA
3656 TGGCCA
MscI 1946 TGGCCA TGG^CCA
3656 TGGCCA
PflMI 195 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2823 CCACCGAGTGG
PshAI 296 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2334 GACGGTCGTC
SacI 1251 GAGCTC GAGCT^C
3139 GAGCTC
SspI 1797 AATATT AAT^ATT
2187 AATATT
StuI 2661 AGGCCT AGG^CCT
4692 AGGCCT
Van91I 195 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2823 CCACCGAGTGG
XmnI 1964 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3217 GAAACTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII