Restriction map for
pF1KB5746
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbvCI BcgI BclI BfrI BlnI
BlpI BpmI Bpu1102I BsaHI BsaWI
BseAI BsgI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HpaI KasI KpnI
KspI MluI MluNI MroI MscI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XcmI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Alw44I 643 GTGCAC G^TGCAC
ApaI 633 GGGCCC GGGCC^C
ApaLI 643 GTGCAC G^TGCAC
Asp700I 1212 GAACCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 95 GGCACC G^G[CT][GA]CC
BbrPI 646 CACGTG CAC^GTG
BbsI 1453 GTCTTC GAAGAC(2/6)
BglII 1914 AGATCT A^GATCT
BpuAI 1453 GTCTTC GAAGAC(2/6)
BsaAI 646 CACGTG [CT]AC^GT[GA]
BsaBI 222 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1687 CGTCTC CGTCTC(1/5)
BspMI 1393 GCAGGT ACCTGC(4/8)
BsrFI 1254 ACCGGC [GA]^CCGG[CT]
BssSI 955 CTCGTG CACGAG(-5/-1)
Cfr10I 1254 ACCGGC [GA]^CCGG[CT]
DraIII 145 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
FauI 1659 GCGGG CCCGC(4/6)
HindIII 2032 AAGCTT A^AGCTT
MamI 222 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI 318 GCATGT [GA]CATG^[CT]
PaeR7I 80 CTCGAG C^TCGAG
PflMI 19 CCAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 646 CACGTG CAC^GTG
PmlI 646 CACGTG CAC^GTG
PpuMI 34 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 340 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1397 GTCGAC G^TCGAC
SmaI 1874 CCCGGG CCC^GGG
Van91I 19 CCAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 80 CTCGAG C^TCGAG
XmaCI 1874 CCCGGG C^CCGGG
XmaI 1874 CCCGGG C^CCGGG
XmnI 1212 GAACCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccI 1270 GTCTAC GT^[AC][GT]AC
1397 GTCGAC
AlwNI 740 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2128 CAGTGCCTG
AvaI 80 CTCGAG C^[CT]CG[GA]G
1874 CCCGGG
BglI 853 GCCTTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1102 GCCATCCTGGC
BsaI 83 GAGACC GGTCTC(1/5)
1825 GAGACC
BsmI 865 GCATTC GAATGC(1/-1)
1992 GCATTC
BsoBI 80 CTCGAG C^[CT]CG[GA]G
1874 CCCGGG
BstXI 584 CCATCACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1046 CCACCCAGTTGG
CfoI 484 GCGC GCG^C
714 GCGC
DsaI 937 CCATGG C^C[GA][CT]GG
2084 CCATGG
HhaI 484 GCGC GCG^C
714 GCGC
HinP1I 484 GCGC G^CGC
714 GCGC
MaeII 428 ACGT A^CGT
647 ACGT
MfeI 56 CAATTG C^AATTG
1928 CAATTG
MslI 588 CACCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1128 CACTCGGATG
MunI 56 CAATTG C^AATTG
1928 CAATTG
NcoI 937 CCATGG C^CATGG
2084 CCATGG
SacI 952 GAGCTC GAGCT^C
1147 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbvI BciVI BcnI
BfaI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII