Restriction map for
pF1KE2653
##################################
# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspHI AvaI AvaII
AviII AvrII BamHI BbrPI BbvCI
BbvI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI BspEI
BspHI BspMI BsrBI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoT22I FauI FbaI Fnu4HI FseI
FspI HgaI HincII HindII HindIII
HpaI ItaI KpnI Ksp632I KspI
MamI MfeI MluI MroI MspA1I
MunI MvnI NaeI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TseI XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AciI 1236 CCGC CCGC(-3/-1)
AcyI 1208 GGCGCC G[GA]^CG[CT]C
AflIII 647 ACATGT A^C[GA][CT]GT
AlwI 1498 GATCC GGATC(4/5)
Aor51HI 918 AGCGCT AGC^GCT
AspI 1186 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1208 GGCGCC G^G[CT][GA]CC
BanII 41 GGGCTC G[GA]GC[CT]^C
BbeI 1208 GGCGCC GGCGC^C
BglII 259 AGATCT A^GATCT
BmyI 41 GGGCTC G[AGT]GC[ACT]^C
BsaHI 1208 GGCGCC G[GA]^CG[CT]C
BsaI 168 GGTCTC GGTCTC(1/5)
BseRI 968 GAGGAG GAGGAG(10/8)
BsiYI 433 CCATATTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 433 CCATATTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 41 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 1004 TGTACA T^GTACA
BspDI 1202 ATCGAT AT^CGAT
BspLU11I 647 ACATGT A^CATGT
BsrGI 1004 TGTACA T^GTACA
BssSI 1052 CACGAG CACGAG(-5/-1)
BstAPI 1510 GCAATTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr13I 1120 GGGCC G^G[ACGT]CC
ClaI 1202 ATCGAT AT^CGAT
Eco47III 918 AGCGCT AGC^GCT
EcoRI 808 GAATTC G^AATTC
EcoRV 484 GATATC GAT^ATC
Hin1I 1208 GGCGCC G[GA]^CG[CT]C
KasI 1208 GGCGCC G^GCGCC
MaeII 1448 ACGT A^CGT
NarI 1208 GGCGCC GG^CGCC
NdeI 346 CATATG CA^TATG
NspI 647 ACATGT [GA]CATG^[CT]
PflFI 1186 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 433 CCATATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau96I 1120 GGGCC G^G[ACGT]CC
SfoI 1208 GGCGCC GGC^GCC
SspBI 1004 TGTACA T^GTACA
Tth111I 1186 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 433 CCATATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 277 GAATTT [GA]^AATT[CT]
808 GAATTC
AfaI 383 GTAC GT^AC
1005 GTAC
AlwNI 381 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
699 CAGTAACTG
ApoI 277 GAATTT [GA]^AATT[CT]
808 GAATTC
BalI 1075 TGGCCA TGG^CCA
1251 TGGCCA
BbsI 536 GTCTTC GAAGAC(2/6)
1373 GTCTTC
BcnI 711 CCCGG CC^[GC]GG
798 CCGGG
BfaI 296 CTAG C^TAG
998 CTAG
BpuAI 536 GTCTTC GAAGAC(2/6)
1373 GTCTTC
BsgI 1428 CTGCAC GTGCAG(16/14)
368 GTGCAG
BsmAI 659 GAGAC GTCTC(1/5)
169 GTCTC
BsmFI 416 GGGAC GGGAC(10/14)
430 GTCCC
BsrDI 1181 GCAATG GCAATG(2/0)
1238 GCAATG
BstYI 259 AGATCT [GA]^GATC[CT]
1497 AGATCC
EcoT14I 146 CCAAGG C^C[AT][AT]GG
214 CCTTGG
HapII 712 CCGG C^CGG
798 CCGG
HinfI 1319 GATTC G^A[ACGT]TC
1522 GAATC
HpaII 712 CCGG C^CGG
798 CCGG
MaeI 296 CTAG C^TAG
998 CTAG
MflI 259 AGATCT [GA]^GATC[CT]
1497 AGATCC
MluNI 1075 TGGCCA TGG^CCA
1251 TGGCCA
MscI 1075 TGGCCA TGG^CCA
1251 TGGCCA
MslI 346 CATATGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1036 CATCCTTATG
MspI 712 CCGG C^CGG
798 CCGG
NciI 711 CCCGG CC^[GC]GG
798 CCGGG
RsaI 383 GTAC GT^AC
1005 GTAC
StyI 146 CCAAGG C^C[AT][AT]GG
214 CCTTGG
TfiI 1319 GATTC G^A[AT]TC
1522 GAATC
TspRI 551 CACTG CA[GC]TG[ACGT][ACGT]^
777 CAGTG
XcmI 433 CCATATTCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1290 CCAAGTGGCTTTTGG
XhoII 259 AGATCT [GA]^GATC[CT]
1497 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AseI AsnI BpmI BsaJI
BsrI BssKI BstF5I BstNI Cac8I
CfoI DdeI DpnII EaeI EcoRII
FokI HaeII HaeIII HhaI HinP1I
HphI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PshBI Sau3AI ScrFI SfaNI
TaqI Tru9I Tsp45I Tsp509I TthHB8I