Restriction map for
pF1KE9635
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# NO CUT ENZYMES ( 68 enzymes) #
##################################
AccI AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspEI BamHI BbeI BbrPI
BcgI BclI BfrI BsiWI Bsp1407I
BspMI BsrGI BssHII Bst1107I BstEII
BstPI BstZ17I CpoI DraI DraIII
Eam1105I Eco47III EcoO65I FbaI FseI
KasI KspI MfeI MluI MunI
NaeI NarI NgoMIV NheI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RsrII SacII SalI SfiI SfoI
SgrAI SnaBI SphI SspBI StuI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 3615 GGTACC G^GTACC
AccIII 559 TCCGGA T^CCGGA
Asp718I 3615 GGTACC G^GTACC
AspI 4501 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2439 TGCGCA TGC^GCA
AvrII 2829 CCTAGG C^CTAGG
BalI 1964 TGGCCA TGG^CCA
BanI 3615 GGTACC G^G[CT][GA]CC
BglII 377 AGATCT A^GATCT
BlnI 2829 CCTAGG C^CTAGG
BsaAI 254 CACGTA [CT]AC^GT[GA]
BsaI 4158 GAGACC GGTCTC(1/5)
BsaWI 559 TCCGGA [AT]^CCGG[AT]
BseAI 559 TCCGGA T^CCGGA
BsiEI 193 CGGCCG CG[GA][CT]^CG
BsmI 2235 GAATGC GAATGC(1/-1)
BspEI 559 TCCGGA T^CCGGA
BspLU11I 363 ACATGT A^CATGT
BsrBI 4264 GAGCGG CCGCTC(-3/-3)
BsrFI 965 ACCGGC [GA]^CCGG[CT]
BstBI 103 TTCGAA TT^CGAA
Cfr10I 965 ACCGGC [GA]^CCGG[CT]
DrdI 922 GACCTGTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 4017 CCATGG C^C[GA][CT]GG
EagI 193 CGGCCG C^GGCCG
EclXI 193 CGGCCG C^GGCCG
Eco52I 193 CGGCCG C^GGCCG
EcoNI 3735 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2474 GATATC GAT^ATC
EcoT22I 2237 ATGCAT ATGCA^T
FspI 2439 TGCGCA TGC^GCA
HaeII 39 GGCGCT [GA]GCGC^[CT]
HpaI 1798 GTTAAC GTT^AAC
KpnI 3615 GGTACC GGTAC^C
MluNI 1964 TGGCCA TGG^CCA
MroI 559 TCCGGA T^CCGGA
MscI 1964 TGGCCA TGG^CCA
NcoI 4017 CCATGG C^CATGG
NdeI 1195 CATATG CA^TATG
NotI 192 GCGGCCGC GC^GGCCGC
NsiI 2237 ATGCAT ATGCA^T
NspV 103 TTCGAA TT^CGAA
PflFI 4501 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3887 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2521 GAGCTC GAGCT^C
SapI 2551 GCTCTTC GCTCTTC(1/4)
SbfI 3078 CCTGCAGG CCTGCA^GG
SexAI 1552 ACCTGGT A^CC[AT]GGT
SfuI 103 TTCGAA TT^CGAA
SmaI 57 CCCGGG CCC^GGG
SpeI 1236 ACTAGT A^CTAGT
Sse8387I 3078 CCTGCAGG CCTGCA^GG
SspI 718 AATATT AAT^ATT
Tth111I 4501 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 908 CCACCAATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 57 CCCGGG C^CCGGG
XmaI 57 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 770 GACGTC GACGT^C
2816 GACGTC
AccII 32 CGCG CG^CG
126 CGCG
AflIII 363 ACATGT A^C[GA][CT]GT
3093 ACGTGT
ApaI 54 GGGCCC GGGCC^C
60 GGGCCC
AspHI 2521 GAGCTC G[AT]GC[AT]^C
2905 GAGCAC
BglI 62 GCCCGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
201 GCCGGATTGGC
BlpI 1579 GCTGAGC GC^T[ACGT]AGC
4129 GCTCAGC
Bpu1102I 1579 GCTGAGC GC^T[ACGT]AGC
4129 GCTCAGC
BsaBI 2474 GATATCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2727 GATGCGTATC
BsiHKAI 2521 GAGCTC G[AT]GC[AT]^C
2905 GAGCAC
BspDI 1865 ATCGAT AT^CGAT
2477 ATCGAT
BspHI 1664 TCATGA T^CATGA
1689 TCATGA
BsrDI 1284 CATTGC GCAATG(2/0)
953 GCAATG
BssSI 3131 CACGAG CACGAG(-5/-1)
290 CTCGTG
BstUI 32 CGCG CG^CG
126 CGCG
CelII 1579 GCTGAGC GC^T[ACGT]AGC
4129 GCTCAGC
ClaI 1865 ATCGAT AT^CGAT
2477 ATCGAT
FauI 30 CCCGC CCCGC(4/6)
63 CCCGC
HgaI 4235 GACGC GACGC(5/10)
4615 GACGC
HincII 1798 GTTAAC GT[CT]^[GA]AC
2508 GTTGAC
HindII 1798 GTTAAC GT[CT]^[GA]AC
2508 GTTGAC
MamI 2474 GATATCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2727 GATGCGTATC
MvnI 32 CGCG CG^CG
126 CGCG
PflMI 3050 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3513 CCAAGATGTGG
RcaI 1664 TCATGA T^CATGA
1689 TCATGA
ScaI 326 AGTACT AGT^ACT
346 AGTACT
SmlI 2935 CTCAAG C^T[CT][GA]AG
4216 CTTGAG
Van91I 3050 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3513 CCAAGATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApoI Asp700I
AvaI AvaII BanII BbsI BbvCI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI Eco81I
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI