Restriction map for
pF1KE2586
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# NO CUT ENZYMES ( 89 enzymes) #
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AatII AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI AspEI AvaI
AviII AvrII BbeI BfrI BlnI
BseAI BsiWI BsoBI BspDI BspEI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraIII DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoT22I
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KspI MfeI
MluI MroI MunI NarI NdeI
NotI NruI NsiI PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI SspI StuI
SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 376 GGTACC G^GTACC
AccI 28 GTAGAC GT^[AC][GT]AC
AccII 949 CGCG CG^CG
AcyI 2140 GGCGTC G[GA]^CG[CT]C
AflIII 512 ACGTGT A^C[GA][CT]GT
ApaI 1565 GGGCCC GGGCC^C
Asp700I 2085 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 376 GGTACC G^GTACC
AspI 2194 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 244 GGATCC G^GATCC
BbrPI 511 CACGTG CAC^GTG
BcgI 831 GCACAAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1278 GTATCC GTATCC(6/5)
BclI 1247 TGATCA T^GATCA
BglII 1631 AGATCT A^GATCT
BsaHI 2140 GGCGTC G[GA]^CG[CT]C
BsaWI 83 ACCGGA [AT]^CCGG[AT]
BsgI 508 CTGCAC GTGCAG(16/14)
BsiEI 626 CGGCCG CG[GA][CT]^CG
BsmBI 2225 GAGACG CGTCTC(1/5)
Bsp1407I 2117 TGTACA T^GTACA
BsrDI 113 GCAATG GCAATG(2/0)
BsrFI 631 GCCGGC [GA]^CCGG[CT]
BsrGI 2117 TGTACA T^GTACA
BstAPI 1785 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 949 CGCG CG^CG
Cfr10I 631 GCCGGC [GA]^CCGG[CT]
DraI 1144 TTTAAA TTT^AAA
EagI 626 CGGCCG C^GGCCG
EclXI 626 CGGCCG C^GGCCG
Eco52I 626 CGGCCG C^GGCCG
EcoNI 1556 CCTATAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1573 GATATC GAT^ATC
FbaI 1247 TGATCA T^GATCA
Hin1I 2140 GGCGTC G[GA]^CG[CT]C
KpnI 376 GGTACC GGTAC^C
MvnI 949 CGCG CG^CG
NaeI 631 GCCGGC GCC^GGC
NgoMIV 631 GCCGGC G^CCGGC
NheI 525 GCTAGC G^CTAGC
PflFI 2194 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1518 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 511 CACGTG CAC^GTG
PmlI 511 CACGTG CAC^GTG
PpuMI 254 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 421 CAGCTG CAG^CTG
SapI 1371 GAAGAGC GCTCTTC(1/4)
SbfI 1782 CCTGCAGG CCTGCA^GG
Sse8387I 1782 CCTGCAGG CCTGCA^GG
SspBI 2117 TGTACA T^GTACA
Tth111I 2194 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1518 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2160 TCTAGA T^CTAGA
XmnI 2085 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 439 AAATTC [GA]^AATT[CT]
1148 AAATTC
ApoI 439 AAATTC [GA]^AATT[CT]
1148 AAATTC
BglI 490 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2132 GCCATGGAGGC
BlpI 2055 GCTGAGC GC^T[ACGT]AGC
2269 GCTGAGC
BpmI 2258 CTCCAG CTGGAG(16/14)
1199 CTGGAG
Bpu1102I 2055 GCTGAGC GC^T[ACGT]AGC
2269 GCTGAGC
BsaBI 1584 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2031 GATGGGCATC
BsmI 1809 GAATGC GAATGC(1/-1)
2164 GAATGC
BspHI 620 TCATGA T^CATGA
1760 TCATGA
BspMI 221 ACCTGC ACCTGC(4/8)
1899 ACCTGC
BstBI 1092 TTCGAA TT^CGAA
1496 TTCGAA
CelII 2055 GCTGAGC GC^T[ACGT]AGC
2269 GCTGAGC
CfoI 1168 GCGC GCG^C
1813 GCGC
DsaI 491 CCATGG C^C[GA][CT]GG
2133 CCATGG
EarI 55 CTCTTC CTCTTC(1/4)
1371 GAAGAG
HhaI 1168 GCGC GCG^C
1813 GCGC
HinP1I 1168 GCGC G^CGC
1813 GCGC
Ksp632I 55 CTCTTC CTCTTC(1/4)
1371 GAAGAG
MamI 1584 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2031 GATGGGCATC
NcoI 491 CCATGG C^CATGG
2133 CCATGG
NspV 1092 TTCGAA TT^CGAA
1496 TTCGAA
RcaI 620 TCATGA T^CATGA
1760 TCATGA
SfuI 1092 TTCGAA TT^CGAA
1496 TTCGAA
Tsp509I 440 AATT ^AATT
1149 AATT
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpuAI BsaAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I TspRI TthHB8I XcmI
XhoII