Restriction map for
pF1KE9396
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# NO CUT ENZYMES ( 30 enzymes) #
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AclI AgeI AscI AviII BbrPI
BsaAI BsiWI Bsp1407I BspDI BsrGI
BssHII ClaI CpoI FspI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I RsrII SfiI SgrAI
SnaBI SphI SspBI SwaI XhoI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AatII 3446 GACGTC GACGT^C
AflII 8010 CTTAAG C^TTAAG
AhdI 6883 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 7865 GTGCAC G^TGCAC
Aor51HI 6188 AGCGCT AGC^GCT
ApaLI 7865 GTGCAC G^TGCAC
AspEI 6883 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2409 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 78 CCTAGG C^CTAGG
BfrI 8010 CTTAAG C^TTAAG
BlnI 78 CCTAGG C^CTAGG
BstEII 10854 GGTGACC G^GT[ACGT]ACC
BstPI 10854 GGTGACC G^GT[ACGT]ACC
DrdI 1668 GACCTCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 6883 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 6188 AGCGCT AGC^GCT
EcoO65I 10854 GGTGACC G^GT[ACGT]ACC
EcoT22I 11689 ATGCAT ATGCA^T
FseI 305 GGCCGGCC GGCCGG^CC
MluI 99 ACGCGT A^CGCGT
NsiI 11689 ATGCAT ATGCA^T
PflFI 2409 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 3451 CGATCG CGAT^CG
SalI 6476 GTCGAC G^TCGAC
SapI 3218 GAAGAGC GCTCTTC(1/4)
SbfI 10819 CCTGCAGG CCTGCA^GG
SexAI 7344 ACCTGGT A^CC[AT]GGT
Sse8387I 10819 CCTGCAGG CCTGCA^GG
Tth111I 2409 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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AseI 958 ATTAAT AT^TAAT
5212 ATTAAT
AsnI 958 ATTAAT AT^TAAT
5212 ATTAAT
BalI 5679 TGGCCA TGG^CCA
8316 TGGCCA
BcgI 6 GCACAGCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
247 GCAGCCTCCTCG
BsaBI 6845 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
9863 GATCTAAATC
BsmBI 1676 CGTCTC CGTCTC(1/5)
9167 CGTCTC
BssSI 524 CTCGTG CACGAG(-5/-1)
11721 CTCGTG
Bst1107I 5036 GTATAC GTA^TAC
6160 GTATAC
BstBI 6434 TTCGAA TT^CGAA
6560 TTCGAA
BstXI 5249 CCAACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
9335 CCAGTGTGATGG
BstZ17I 5036 GTATAC GTA^TAC
6160 GTATAC
DraIII 9332 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
9908 CACAGAGTG
EcoRV 6143 GATATC GAT^ATC
6244 GATATC
HpaI 9241 GTTAAC GTT^AAC
11140 GTTAAC
KspI 91 CCGCGG CCGC^GG
185 CCGCGG
MamI 6845 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
9863 GATCTAAATC
MfeI 3344 CAATTG C^AATTG
9307 CAATTG
MluNI 5679 TGGCCA TGG^CCA
8316 TGGCCA
MscI 5679 TGGCCA TGG^CCA
8316 TGGCCA
MunI 3344 CAATTG C^AATTG
9307 CAATTG
NaeI 306 GCCGGC GCC^GGC
11575 GCCGGC
NdeI 5764 CATATG CA^TATG
7249 CATATG
NgoMIV 306 GCCGGC G^CCGGC
11575 GCCGGC
NotI 181 GCGGCCGC GC^GGCCGC
232 GCGGCCGC
NspV 6434 TTCGAA TT^CGAA
6560 TTCGAA
PshBI 958 ATTAAT AT^TAAT
5212 ATTAAT
SacII 91 CCGCGG CCGC^GG
185 CCGCGG
ScaI 5548 AGTACT AGT^ACT
6757 AGTACT
SfuI 6434 TTCGAA TT^CGAA
6560 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 170 enzymes) #
#########################################
Acc65I AccI AccII AccIII AciI
AcsI AcyI AfaI AflIII AluI
AlwI AlwNI AocI ApaI ApoI
Asp700I Asp718I AspHI AvaI AvaII
BamHI BanI BanII BbeI BbsI
BbvCI BbvI BciVI BclI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI KasI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MroI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NheI NlaIII NlaIV NspI PflMI
PleI PpuMI PshAI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI SpeI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XcmI XhoII XmaCI XmaI XmnI