Restriction map for
pF1KB8272
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BanII
BbrPI BbsI BbvCI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmAI BsmBI BsmFI BsmI
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DdeI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MaeI MaeIII MamI
MfeI MflI MluI MroI MseI
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TfiI Tru9I
Tsp45I Tsp509I Tth111I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AfaI 132 GTAC GT^AC
AflIII 5 ACGTGT A^C[GA][CT]GT
Alw44I 349 GTGCAC G^TGCAC
AlwI 18 GATCC GGATC(4/5)
AlwNI 124 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 349 GTGCAC G^TGCAC
AspHI 349 GTGCAC G[AT]GC[AT]^C
AvaI 321 CTCGGG C^[CT]CG[GA]G
BalI 377 TGGCCA TGG^CCA
BanI 23 GGCGCC G^G[CT][GA]CC
BbeI 23 GGCGCC GGCGC^C
BcgI 65 GCACGGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 237 CCGGG CC^[GC]GG
BglI 232 GCCTTCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 349 GTGCAC G[AGT]GC[ACT]^C
BpmI 399 CTGGAG CTGGAG(16/14)
BsiHKAI 349 GTGCAC G[AT]GC[AT]^C
BsoBI 321 CTCGGG C^[CT]CG[GA]G
Bsp1286I 349 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 131 TGTACA T^GTACA
BspHI 14 TCATGA T^CATGA
BspMI 353 ACCTGC ACCTGC(4/8)
BsrGI 131 TGTACA T^GTACA
BstBI 73 TTCGAA TT^CGAA
BstXI 405 CCAGTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 18 GATC ^GATC
EcoT14I 53 CCAAGG C^C[AT][AT]GG
FauI 285 CCCGC CCCGC(4/6)
HaeII 23 GGCGCC [GA]GCGC^[CT]
HgaI 49 GACGC GACGC(5/10)
HphI 185 TCACC GGTGA(8/7)
KasI 23 GGCGCC G^GCGCC
MaeII 5 ACGT A^CGT
MboI 18 GATC ^GATC
MboII 41 TCTTC GAAGA(8/7)
MluNI 377 TGGCCA TGG^CCA
MscI 377 TGGCCA TGG^CCA
MslI 352 CACCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 124 CAGCGG C[AC]G^C[GT]G
NarI 23 GGCGCC GG^CGCC
NciI 237 CCGGG CC^[GC]GG
NdeII 18 GATC ^GATC
NspV 73 TTCGAA TT^CGAA
RcaI 14 TCATGA T^CATGA
RsaI 132 GTAC GT^AC
Sau3AI 18 GATC ^GATC
SfoI 23 GGCGCC GGC^GCC
SfuI 73 TTCGAA TT^CGAA
SmlI 103 CTCAAG C^T[CT][GA]AG
SspBI 131 TGTACA T^GTACA
StyI 53 CCAAGG C^C[AT][AT]GG
XcmI 218 CCACAGTGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 23 GGCGCC G[GA]^CG[CT]C
49 GACGCC
AluI 293 AGCT AG^CT
397 AGCT
AvaII 451 GGACC G^G[AT]CC
462 GGTCC
BbvI 123 GCAGC GCAGC(8/12)
294 GCTGC
BsaHI 23 GGCGCC G[GA]^CG[CT]C
49 GACGCC
BsaJI 53 CCAAGG C^C[ACGT][ACGT]GG
314 CCCAGG
BstNI 315 CCAGG CC^[AT]GG
412 CCTGG
Cac8I 228 GCTGGC GC[ACGT]^[ACGT]GC
284 GCCCGC
CfoI 24 GCGC GCG^C
84 GCGC
DraII 209 AGGCCCC [GA]G^G[ACGT]CC[CT]
257 AGGCCCT
EaeI 202 CGGCCA [CT]^GGCC[GA]
377 TGGCCA
EcoO109I 209 AGGCCCC [GA]G^G[ACGT]CC[CT]
257 AGGCCCT
EcoRII 315 CCAGG ^CC[AT]GG
412 CCTGG
HapII 21 CCGG C^CGG
237 CCGG
HhaI 24 GCGC GCG^C
84 GCGC
Hin1I 23 GGCGCC G[GA]^CG[CT]C
49 GACGCC
HinP1I 24 GCGC G^CGC
84 GCGC
HinfI 58 GAGTC G^A[ACGT]TC
319 GACTC
HpaII 21 CCGG C^CGG
237 CCGG
MspI 21 CCGG C^CGG
237 CCGG
MvaI 315 CCAGG CC^[AT]GG
412 CCTGG
NspI 357 GCATGT [GA]CATG^[CT]
424 GCATGT
PflMI 144 CCAACTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
369 CCAGACGATGG
PleI 319 GACTC GAGTC(4/5)
58 GAGTC
TseI 123 GCAGC G^C[AT]GC
294 GCTGC
Van91I 144 CCAACTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
369 CCAGACGATGG
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI BsiYI BslI BsrI BssKI
BstF5I Cfr13I Fnu4HI FokI HaeIII
ItaI MnlI MwoI NlaIII NlaIV
Sau96I ScrFI SfaNI TaqI TspRI
TthHB8I