Restriction map for
pF1KE2660
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AccIII AclI AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
AvaI BalI BbeI BbrPI BbvCI
BglII BseAI BsgI BsiWI BsmBI
BsoBI BspDI BspEI BsrBI BsrDI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI FauI
FseI HindIII HpaI KasI KspI
MluI MluNI MroI MscI NarI
NdeI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AatII 568 GACGTC GACGT^C
Acc65I 1427 GGTACC G^GTACC
AccI 781 GTCGAC GT^[AC][GT]AC
AcyI 568 GACGTC G[GA]^CG[CT]C
AhdI 21 GACTTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 583 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 494 GGGCCC GGGCC^C
Asp718I 1427 GGTACC G^GTACC
AspEI 21 GACTTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1692 GAGCTC G[AT]GC[AT]^C
AspI 1818 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 991 TGCGCA TGC^GCA
AvrII 1339 CCTAGG C^CTAGG
BamHI 2059 GGATCC G^GATCC
BcgI 335 GCATCATCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 443 CCCGG CC^[GC]GG
BglI 438 GCCCACCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1339 CCTAGG C^CTAGG
BlpI 1071 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1071 GCTGAGC GC^T[ACGT]AGC
BsaAI 1012 TACGTG [CT]AC^GT[GA]
BsaHI 568 GACGTC G[GA]^CG[CT]C
BsaI 1087 GAGACC GGTCTC(1/5)
BsaWI 366 TCCGGT [AT]^CCGG[AT]
BsiEI 2320 CGGTCG CG[GA][CT]^CG
BsiHKAI 1692 GAGCTC G[AT]GC[AT]^C
BsmI 2164 GCATTC GAATGC(1/-1)
Bsp1407I 1637 TGTACA T^GTACA
BspHI 1061 TCATGA T^CATGA
BspLU11I 1796 ACATGT A^CATGT
BsrFI 1947 GCCGGC [GA]^CCGG[CT]
BsrGI 1637 TGTACA T^GTACA
BssHII 652 GCGCGC G^CGCGC
BssSI 1135 CACGAG CACGAG(-5/-1)
BstYI 2059 GGATCC [GA]^GATC[CT]
CelII 1071 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1947 GCCGGC [GA]^CCGG[CT]
EaeI 626 TGGCCG [CT]^GGCC[GA]
Eam1105I 21 GACTTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 168 GATATC GAT^ATC
FspI 991 TGCGCA TGC^GCA
HaeII 321 GGCGCT [GA]GCGC^[CT]
HgaI 1079 GCGTC GACGC(5/10)
Hin1I 568 GACGTC G[GA]^CG[CT]C
KpnI 1427 GGTACC GGTAC^C
MfeI 899 CAATTG C^AATTG
MflI 2059 GGATCC [GA]^GATC[CT]
MspA1I 2333 CAGCTG C[AC]G^C[GT]G
MunI 899 CAATTG C^AATTG
NaeI 1947 GCCGGC GCC^GGC
NciI 443 CCCGG CC^[GC]GG
NcoI 2340 CCATGG C^CATGG
NgoMIV 1947 GCCGGC G^CCGGC
NheI 633 GCTAGC G^CTAGC
PflFI 1818 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 470 CCATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 2333 CAGCTG CAG^CTG
RcaI 1061 TCATGA T^CATGA
SacI 1692 GAGCTC GAGCT^C
SalI 781 GTCGAC G^TCGAC
SphI 2083 GCATGC GCATG^C
SspBI 1637 TGTACA T^GTACA
Tth111I 1818 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 470 CCATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1664 CCATCGAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 2059 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 653 CGCG CG^CG
655 CGCG
AflII 1902 CTTAAG C^TTAAG
2232 CTTAAG
BanII 494 GGGCCC G[GA]GC[CT]^C
1692 GAGCTC
BbsI 766 GAAGAC GAAGAC(2/6)
916 GTCTTC
BclI 500 TGATCA T^GATCA
1572 TGATCA
BfrI 1902 CTTAAG C^TTAAG
2232 CTTAAG
BpuAI 766 GAAGAC GAAGAC(2/6)
916 GTCTTC
BspMI 575 ACCTGC ACCTGC(4/8)
587 ACCTGC
BstAPI 238 GCATCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
582 GCAGAACCTGC
BstUI 653 CGCG CG^CG
655 CGCG
BstXI 410 CCATCGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1664 CCATCGAGATGG
DsaI 614 CCGTGG C^C[GA][CT]GG
2340 CCATGG
EcoT22I 119 ATGCAT ATGCA^T
754 ATGCAT
FbaI 500 TGATCA T^GATCA
1572 TGATCA
HincII 571 GTCAAC GT[CT]^[GA]AC
781 GTCGAC
HindII 571 GTCAAC GT[CT]^[GA]AC
781 GTCGAC
MvnI 653 CGCG CG^CG
655 CGCG
NsiI 119 ATGCAT ATGCA^T
754 ATGCAT
NspI 1796 ACATGT [GA]CATG^[CT]
2083 GCATGC
PpuMI 1816 AGGACCC [GA]G^G[AT]CC[CT]
2365 AGGTCCC
SapI 1471 GAAGAGC GCTCTTC(1/4)
2139 GAAGAGC
ScaI 1055 AGTACT AGT^ACT
2204 AGTACT
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AvaII BanI
BbvI BciVI BfaI BmyI BpmI
BsaBI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI